##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546375_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	876910
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13791038989178	31.0	31.0	33.0	30.0	34.0
2	31.458106305094024	31.0	31.0	34.0	30.0	34.0
3	31.477483436156504	31.0	31.0	34.0	30.0	34.0
4	35.32587038578645	37.0	35.0	37.0	33.0	37.0
5	35.23270917197888	37.0	35.0	37.0	33.0	37.0
6	35.28504521558655	37.0	35.0	37.0	32.0	37.0
7	35.77754159491852	37.0	35.0	37.0	35.0	37.0
8	35.71556602159857	37.0	35.0	37.0	35.0	37.0
9	37.49518308606356	39.0	37.0	39.0	35.0	39.0
10	36.9861741797904	39.0	37.0	39.0	33.0	39.0
11	36.56415253560799	39.0	35.0	39.0	32.0	39.0
12	35.20600631763807	35.0	35.0	39.0	31.0	39.0
13	34.510456033116284	35.0	33.0	39.0	27.0	39.0
14	35.268601110718315	36.0	33.0	40.0	27.0	41.0
15	35.8057474541287	36.0	34.0	40.0	30.0	41.0
16	36.08493802100558	36.0	34.0	40.0	31.0	41.0
17	36.05358132533555	36.0	34.0	40.0	31.0	41.0
18	35.98053049913902	36.0	35.0	40.0	31.0	41.0
19	35.80538709787778	36.0	35.0	40.0	31.0	41.0
20	35.57015657251029	35.0	34.0	40.0	30.0	41.0
21	35.36681643498193	35.0	34.0	40.0	30.0	41.0
22	35.24000752642802	35.0	34.0	40.0	30.0	41.0
23	35.263810425243186	35.0	34.0	40.0	30.0	41.0
24	35.18696103362945	35.0	34.0	40.0	30.0	41.0
25	35.115666373972246	35.0	34.0	40.0	30.0	41.0
26	34.98543065993089	35.0	34.0	39.0	29.0	41.0
27	34.847108597233465	35.0	34.0	39.0	29.0	41.0
28	34.868871377906515	35.0	34.0	39.0	29.0	41.0
29	34.888858605786226	35.0	34.0	39.0	29.0	41.0
30	34.77833529096487	35.0	34.0	39.0	29.0	41.0
31	34.42925043619071	35.0	34.0	39.0	27.0	41.0
32	34.122444720666884	35.0	33.0	39.0	25.0	41.0
33	33.86985665575715	35.0	33.0	39.0	24.0	41.0
34	33.64360538709788	35.0	33.0	39.0	23.0	41.0
35	33.42493642449054	35.0	33.0	39.0	23.0	41.0
36	33.14928898062515	35.0	33.0	39.0	21.0	41.0
37	32.89486948489583	35.0	32.0	39.0	21.0	41.0
38	32.889092381202175	35.0	32.0	39.0	21.0	41.0
39	32.90764046481395	35.0	32.0	38.0	21.0	41.0
40	32.62214594428163	35.0	31.0	38.0	18.0	41.0
41	32.605273061089505	35.0	32.0	38.0	18.0	41.0
42	32.53743029501317	35.0	32.0	38.0	18.0	41.0
43	32.414670832810664	35.0	31.0	38.0	18.0	40.0
44	32.252478589593004	35.0	31.0	38.0	18.0	40.0
45	32.19782417807985	35.0	31.0	38.0	18.0	40.0
46	32.169297875494635	35.0	31.0	38.0	18.0	40.0
47	32.09421035225964	35.0	31.0	38.0	18.0	40.0
48	32.08528355247403	35.0	31.0	38.0	18.0	40.0
49	32.150222941921065	35.0	31.0	37.0	18.0	40.0
50	31.94816571826071	35.0	31.0	37.0	18.0	40.0
51	31.690778985300657	35.0	31.0	37.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	4.0
14	11.0
15	32.0
16	147.0
17	389.0
18	867.0
19	1627.0
20	2797.0
21	4393.0
22	6253.0
23	8849.0
24	12336.0
25	16546.0
26	20965.0
27	23281.0
28	24332.0
29	25887.0
30	30074.0
31	35622.0
32	44486.0
33	58216.0
34	108689.0
35	157207.0
36	50377.0
37	58425.0
38	79647.0
39	105423.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.56742425106339	23.441972380289883	29.057942092118914	14.932661276527808
2	30.809661196702052	25.97222063837794	30.030333785679257	13.187784379240744
3	27.63875426212496	23.778038795315368	34.48267211002269	14.100534832536976
4	24.105210340855958	28.680366286163917	32.866770820266616	14.347652552713505
5	22.702101698007777	32.15426896716881	32.03646896488807	13.107160369935341
6	20.513393620782065	40.97410224538436	28.04905862631285	10.463445507520726
7	84.83014220387497	5.216840952891403	8.132761628901482	1.8202552143321435
8	86.76317980180406	4.03690230468349	7.123878163095415	2.0760397304170324
9	84.17887810607702	4.638218289220102	8.833175582442896	2.349728022259981
10	60.90636439315323	17.928521741113684	11.852755698988492	9.312358166744591
11	55.91451802351438	16.936401683182993	17.394259388078595	9.754820905224024
12	49.31600734396916	19.929183154485635	20.623325084672313	10.131484416872883
13	20.92392605854649	47.81927449795304	21.13398182253595	10.122817620964524
14	12.198743314593289	50.88013593185161	25.79170040254986	11.129420351005233
15	10.0959049389333	21.85674698657787	56.82590003535141	11.221448039137426
16	11.623541754570024	14.895257209975938	55.157427786203826	18.32377324925021
17	13.172389412824579	14.694438425836175	28.654137824862303	43.47903433647694
18	21.42660022123137	21.024734579375306	37.0390347926241	20.509630406769226
19	31.03591018462556	22.918771595716777	24.64768334264634	21.397634877011324
20	33.580527078035374	21.573137494155613	23.124722035328595	21.721613392480414
21	20.47792817963075	29.477255362579967	27.99546133582694	22.049355121962343
22	21.764377188080875	24.032112759576236	24.905292447343513	29.298217604999373
23	17.396540123843952	32.970886407955206	23.31733017071307	26.31524329748777
24	19.836813355988642	22.685566363708933	41.505399641924484	15.97222063837794
25	14.091525926263811	24.397030482033504	39.71353958787105	21.797904003831636
26	13.968822342087558	37.1843176608774	28.189437912670627	20.657422084364416
27	17.20758116568405	34.49316349454334	29.606573080475762	18.69268225929685
28	11.685919877752562	28.628821657866826	44.11193851136377	15.573319953016842
29	12.875779726539783	23.95513792749541	42.499914472408804	20.66916787355601
30	17.610473138634525	30.013570377803877	33.197477506243516	19.178478977318083
31	31.757991127937874	25.067680833837	23.563649633371725	19.610678404853406
32	33.068501898712526	26.24933003386893	25.22778848456512	15.454379582853427
33	29.97502594336933	26.31216430420454	24.697517419119407	19.015292333306725
34	18.68173472762313	28.64889213260198	28.383642563090856	24.28573057668404
35	19.068889623792636	25.887833415059696	31.969301296598285	23.073975664549383
36	34.48426862505845	21.647375443318015	25.861148806605012	18.00720712501853
37	19.803628650602683	32.639153390883905	29.30870898952002	18.248508968993395
38	20.153151406643783	34.83265101321686	22.12496151258396	22.889236067555395
39	21.06681415424616	32.54165193691485	25.65200533692169	20.7395285719173
40	25.099269024187205	25.04464540260688	23.057554367038808	26.79853120616711
41	16.11750350663124	22.937017481839643	25.516643669247703	35.42883534228142
42	23.25073268636462	24.597621192596733	22.77679579432325	29.374850326715396
43	22.890718545802876	26.58938773648379	24.364073850224084	26.15581986748925
44	20.687071649314067	30.74500233775416	28.053962208208365	20.513963804723403
45	16.25514591007059	39.647740361040476	19.97992952526485	24.117184203624088
46	23.737213625115462	32.16943586000844	25.323579386710154	18.769771128165946
47	22.613381076735354	26.254347652552713	26.265637294591237	24.866633976120696
48	24.396574334880434	22.079232760488534	30.56288558689033	22.961307317740705
49	20.26182846586309	21.737806616414453	32.90862232156094	25.09174259616152
50	18.695077031850474	33.93962892429098	26.014642323613597	21.35065172024495
51	18.309176540351917	35.830016763407876	22.12005793068844	23.740748765551768
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	754.0
1	810.0
2	866.0
3	3433.0
4	6000.0
5	4898.0
6	3796.0
7	4278.0
8	4760.0
9	5307.0
10	5854.0
11	6011.5
12	6169.0
13	6083.5
14	5998.0
15	5597.5
16	5197.0
17	4932.5
18	4668.0
19	4441.0
20	4214.0
21	3949.0
22	3684.0
23	3748.5
24	3813.0
25	3967.5
26	3876.0
27	3630.0
28	4417.0
29	5204.0
30	5541.5
31	5879.0
32	8073.5
33	10268.0
34	10723.5
35	11179.0
36	13127.5
37	15076.0
38	21351.0
39	27626.0
40	47034.0
41	66442.0
42	84268.0
43	102094.0
44	102670.0
45	103246.0
46	95073.5
47	86901.0
48	83633.5
49	80366.0
50	75529.0
51	70692.0
52	63283.5
53	55875.0
54	48577.0
55	41279.0
56	37905.5
57	34532.0
58	29704.0
59	24876.0
60	24256.5
61	23637.0
62	20054.0
63	16471.0
64	14507.0
65	12543.0
66	9829.5
67	7116.0
68	5867.0
69	4618.0
70	3991.0
71	3364.0
72	2595.5
73	1827.0
74	1488.5
75	852.0
76	554.0
77	445.0
78	336.0
79	213.0
80	90.0
81	87.0
82	84.0
83	55.5
84	27.0
85	22.0
86	17.0
87	12.5
88	8.0
89	6.5
90	5.0
91	3.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	876910.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.916007492072776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.67006721934202	18.944678797686016
2	7.59555647139243	3.4811965800978153
3	2.455469884734882	1.688086988254309
4	1.145337738705476	1.0498627280451354
5	0.6274335703899753	0.7189136199917323
6	0.41329628063769425	0.5682660398123527
7	0.2889659612333126	0.4635362292803622
8	0.2294561781048913	0.420657559724326
9	0.19266432693775865	0.3973587443604752
>10	2.1264241848082377	11.714099832419118
>50	1.027421728559272	17.41094356544405
>100	1.2229068360369533	41.57197038044148
>500	0.003499733381953668	0.489104918737611
>1k	0.0014998857351230007	1.0813240157052173
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4623	0.5271920721624795	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3390	0.38658471222816476	No Hit
ACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCTTGTCGGCT	1425	0.16250242328175069	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08005382536406244	0.0
2	0.0	0.0	0.0	0.2774515058557891	0.0
3	0.0	0.0	0.0	0.3846460868276106	0.0
4	0.0	0.0	0.0	0.5893421217684826	0.0
5	0.0	0.0	0.0	0.9373823995620987	0.0
6	0.0	0.0	0.0	1.299449202312666	0.0
7	0.0	0.0	0.0	1.525812227024438	0.0
8	0.0	0.0	0.0	2.159514659429132	0.0
9	0.0	0.0	0.0	2.411079814348109	0.0
10	0.0	0.0	0.0	2.875209542598442	0.0
11	0.0	0.0	0.0	3.210933847259126	0.0
12	0.0	0.0	0.0	3.5604566033002247	0.0
13	0.0	0.0	0.0	3.690800652290429	0.0
14	0.0	0.0	0.0	3.7390382137277487	0.0
15	0.0	0.0	0.0	3.828785166094582	0.0
16	0.0	0.0	0.0	4.042147996943814	0.0
17	0.0	0.0	0.0	4.2655460651606205	0.0
18	0.0	0.0	0.0	4.715649268454003	0.0
19	0.0	0.0	0.0	4.836186153653169	0.0
20	0.0	0.0	0.0	4.994355178980739	0.0
21	0.0	0.0	0.0	5.150813652484292	0.0
22	0.0	0.0	0.0	5.283894584392925	0.0
23	0.0	0.0	0.0	5.445370676580265	0.0
24	0.0	0.0	0.0	5.552337183975551	0.0
25	0.0	0.0	0.0	5.634785781893239	0.0
26	0.0	0.0	0.0	5.726585396448894	0.0
27	0.0	0.0	0.0	5.815420054509585	0.0
28	0.0	0.0	0.0	5.9052810436646865	0.0
29	0.0	0.0	0.0	6.000729835444915	0.0
30	0.0	0.0	0.0	6.143275820779784	0.0
31	0.0	0.0	0.0	6.280234003489526	0.0
32	0.0	0.0	0.0	6.380130230012202	0.0
33	0.0	0.0	0.0	6.489377473172846	0.0
34	0.0	0.0	0.0	6.604668666111688	0.0
35	0.0	0.0	0.0	6.768539530852653	0.0
36	0.0	0.0	0.0	6.885541275615513	0.0
37	0.0	0.0	0.0	7.0011745789191595	0.0
38	0.0	0.0	0.0	7.116123661493198	0.0
39	0.0	0.0	0.0	7.248406335883956	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	20	7.0323696E-4	45.000004	1
GTCATAG	20	7.0323696E-4	45.000004	1
CTAGAGT	20	7.0323696E-4	45.000004	27
CGAACGG	20	7.0323696E-4	45.000004	2
TCCGCTA	20	7.0323696E-4	45.000004	22
CGAGTCA	20	7.0323696E-4	45.000004	13
TTTCGAT	20	7.0323696E-4	45.000004	36
CGTTGAT	40	6.8121153E-9	45.000004	25
GACCGAC	20	7.0323696E-4	45.000004	9
CCGTGAC	20	7.0323696E-4	45.000004	45
ATTCGGC	20	7.0323696E-4	45.000004	17
TGTATCG	20	7.0323696E-4	45.000004	1
CACTGAT	20	7.0323696E-4	45.000004	33
TAACGCG	20	7.0323696E-4	45.000004	1
GACCCTA	20	7.0323696E-4	45.000004	38
ACTTAAC	135	0.0	45.000004	35
TCGATAG	35	1.2114833E-7	45.000004	1
GTGGACG	20	7.0323696E-4	45.000004	1
CCCGTTC	20	7.0323696E-4	45.000004	17
TCTACCA	20	7.0323696E-4	45.000004	27
>>END_MODULE