FastQCFastQC Report
Thu 26 May 2016
SRR1546374_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546374_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1535408
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79390.5170612631951897No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC29160.18991694715671664No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG15460.10068984921271741No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT1000.045.0000044
TACGCTA253.8914193E-545.00000435
GCGATCG253.8914193E-545.0000049
ACGTTAT207.034028E-445.028
AATATCG207.034028E-445.01
CGTAAAT650.044.99999616
GTACGAG1500.043.51
CGTTTTT63750.043.1294141
CGGTCTA2050.042.8048831
CGACGGT2200.040.90909228
CGCATCG1050.040.71428721
ACGGGTA1900.040.263165
ATAGGGC7100.039.9295774
GTTAGCG850.039.7058831
ATTAGCG1200.039.3750041
GTATACG800.039.3751
TAGTCGG403.4592267E-739.3752
ACGGTCT2300.039.13043230
ATCGTAG750.039.01
TGATTCG750.039.015
Produced by FastQC (version 0.11.5)