##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546374_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1535408
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.125642174588123	31.0	31.0	33.0	30.0	34.0
2	31.460098553609203	31.0	31.0	34.0	30.0	34.0
3	31.51864325312881	31.0	31.0	34.0	30.0	34.0
4	35.37361014140867	37.0	35.0	37.0	33.0	37.0
5	35.273632155101446	37.0	35.0	37.0	33.0	37.0
6	35.317649119973325	37.0	35.0	37.0	32.0	37.0
7	35.793528495357585	37.0	35.0	37.0	35.0	37.0
8	35.70984389816908	37.0	35.0	37.0	35.0	37.0
9	37.463678058209936	39.0	37.0	39.0	35.0	39.0
10	36.89709770953388	39.0	37.0	39.0	32.0	39.0
11	36.596190719339745	39.0	35.0	39.0	32.0	39.0
12	35.98014990152455	37.0	35.0	39.0	31.0	39.0
13	35.69312130717047	37.0	35.0	39.0	30.0	39.0
14	36.54629648927191	38.0	35.0	41.0	31.0	41.0
15	36.81940826151746	38.0	35.0	41.0	31.0	41.0
16	36.920279170096805	38.0	35.0	41.0	32.0	41.0
17	36.87158918020487	38.0	35.0	41.0	32.0	41.0
18	36.791936736033676	38.0	35.0	40.0	31.0	41.0
19	36.67777359503142	38.0	35.0	40.0	31.0	41.0
20	36.5121524702229	38.0	35.0	40.0	31.0	41.0
21	36.36652993862218	38.0	35.0	40.0	31.0	41.0
22	36.26983642132905	38.0	35.0	40.0	30.0	41.0
23	36.22094257682649	38.0	35.0	40.0	30.0	41.0
24	36.20752920396403	38.0	35.0	40.0	30.0	41.0
25	36.136316861707115	38.0	35.0	40.0	30.0	41.0
26	36.02465077686192	38.0	35.0	40.0	30.0	41.0
27	35.888538421058115	38.0	34.0	40.0	30.0	41.0
28	35.84716765836833	37.0	34.0	40.0	30.0	41.0
29	35.756591081979515	37.0	34.0	40.0	30.0	41.0
30	35.58249729062243	37.0	34.0	40.0	29.0	41.0
31	35.35226793139022	37.0	34.0	40.0	28.0	41.0
32	35.17720110876067	37.0	34.0	40.0	27.0	41.0
33	34.975042464283106	37.0	34.0	40.0	26.0	41.0
34	34.74059403103279	37.0	34.0	40.0	25.0	41.0
35	34.55087572814522	37.0	33.0	40.0	24.0	41.0
36	34.32911252253473	37.0	33.0	40.0	23.0	41.0
37	34.118957306461866	36.0	33.0	40.0	23.0	41.0
38	34.0126174932005	36.0	33.0	40.0	23.0	41.0
39	33.95376017319175	36.0	33.0	40.0	23.0	41.0
40	33.694517027395975	36.0	33.0	40.0	21.0	41.0
41	33.59701981492867	36.0	32.0	40.0	21.0	41.0
42	33.49043251044674	36.0	32.0	40.0	21.0	41.0
43	33.44111793086919	35.0	32.0	40.0	21.0	41.0
44	33.28391997436512	35.0	32.0	40.0	20.0	41.0
45	33.271105790773525	35.0	32.0	40.0	20.0	41.0
46	33.19724659504184	35.0	32.0	39.0	20.0	41.0
47	33.10935204193283	35.0	32.0	39.0	20.0	41.0
48	33.0215037306045	35.0	32.0	39.0	20.0	41.0
49	33.01151680856163	35.0	32.0	39.0	20.0	41.0
50	32.853575727103156	35.0	31.0	39.0	20.0	41.0
51	32.631127361587275	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	3.0
11	4.0
12	2.0
13	12.0
14	20.0
15	64.0
16	190.0
17	566.0
18	1202.0
19	2380.0
20	3939.0
21	5958.0
22	8894.0
23	12654.0
24	17451.0
25	24091.0
26	30771.0
27	34699.0
28	37367.0
29	40932.0
30	46992.0
31	55986.0
32	68105.0
33	86848.0
34	138246.0
35	181695.0
36	116689.0
37	146904.0
38	201996.0
39	270700.0
40	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.2710159123829	23.140298865187624	27.355465127184438	14.233220095245041
2	32.67483300855538	25.102578598001312	29.437257067828227	12.785331325615083
3	30.062758563196233	25.12706720298448	31.683630670154123	13.126543563665164
4	26.44150610130988	27.792091743692882	30.931322488875924	14.835079666121317
5	22.593603784792055	31.741660848452007	30.417452559840775	15.247282806915166
6	20.5746615883205	41.573054197972134	27.73822983858362	10.114054375123747
7	87.01439617352521	3.779190938174088	7.550305847045215	1.656107041255484
8	88.05288236090993	3.075078415639361	6.940826151745985	1.9312130717047196
9	84.02346477288121	4.4481336556797935	8.81687473297	2.7115268384689934
10	46.277276137678065	25.921448891760367	14.980187676500318	12.821087294061254
11	38.41539186978314	23.976167898044036	22.792378312474597	14.816061919698218
12	35.659577128685015	22.04091681168784	26.90359826183008	15.395907797797067
13	24.825714077300624	31.972739493346392	27.589474589164574	15.612071840188408
14	19.43346654439732	35.10728093119223	29.269809718328936	16.189442806081512
15	17.400716942988442	24.169145920823652	41.428141575398854	17.001995560789055
16	19.72628773589821	19.423827412648624	40.146462699165305	20.70342215228786
17	20.77701822577452	19.542558069255858	29.337088252764087	30.34333545220554
18	23.508800266769484	23.123625772433126	32.772136135802334	20.59543782499505
19	27.865101653762387	24.791716599105907	26.069813365567978	21.273368381563728
20	28.883332638621134	24.594440044600525	25.602120087950563	20.920107228827778
21	24.52813844919396	26.698375936558882	28.89153892646124	19.881946687785916
22	23.74678261413253	23.213699550868565	27.389202088304863	25.650315746694037
23	20.792779508769005	28.558858622594123	26.691081458478788	23.957280410158084
24	21.55615966570449	25.59905901232767	33.91795535779415	18.92682596417369
25	21.556485312047354	24.647520398486915	31.313956941737963	22.482037347727772
26	19.58547825724498	29.754762252118006	27.294829778143658	23.364929712493357
27	20.109117576565968	28.66781988891552	29.616883590550525	21.606178943967986
28	18.53487802590582	27.115854548107084	34.60226858268291	19.74699884330419
29	19.29819305357273	24.73798495253379	34.22920813230099	21.73461386159249
30	22.423811781624167	27.093775726061086	30.68096558048414	19.8014469118306
31	27.857676917145152	25.45864030928587	26.060043975282138	20.62363879828684
32	28.09350999864531	26.701437012181778	25.959158738263703	19.245894250909203
33	26.751781936788138	25.88901451601138	26.281353229890687	21.077850317309796
34	21.169096422579535	27.037048133134643	28.848748997009267	22.945106447276554
35	22.350085449600368	26.070594916790846	29.396551274970562	22.182768358638224
36	28.771961589362565	25.185097381282368	25.837367006033574	20.20557402332149
37	22.244055000364725	29.81884945239311	27.64216416743953	20.294931379802634
38	23.09842074549566	29.732943947146296	24.7501641257568	22.418471181601245
39	22.580968706689035	27.874740785511083	26.13377030730594	23.410520200493938
40	24.576529495743152	25.026963517188918	26.724492773256358	23.672014213811572
41	21.274996613278034	24.140489042651854	27.29274564154935	27.291768702520763
42	24.243328157727458	25.676888488271523	25.435845065285577	24.643938288715443
43	23.74456821900107	26.425875076852538	25.826620676719152	24.00293602742724
44	22.38330137657222	28.214780696726866	26.9061383033044	22.495779623396515
45	20.276174150453823	31.137652011712845	24.873779477506954	23.712394360326375
46	23.272511280389317	28.900266248449924	27.08706741139814	20.740155059762618
47	23.61027166720507	27.14451142627888	27.308311536738117	21.936905369777936
48	23.526385169283994	25.952515552869336	28.175116972166354	22.345982305680316
49	23.017399935391765	24.33034086054	29.719331930014693	22.93292727405354
50	21.65951981492867	28.39134614382627	27.664047601679815	22.285086439565248
51	21.132558902910496	30.261467961610204	25.683661932203037	22.922311203276262
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	646.0
1	1182.0
2	1718.0
3	7916.0
4	14114.0
5	10500.5
6	6887.0
7	7033.0
8	7179.0
9	7362.5
10	7546.0
11	7571.0
12	7596.0
13	7468.5
14	7341.0
15	7037.5
16	6734.0
17	6644.5
18	6555.0
19	6209.5
20	5864.0
21	6056.5
22	6249.0
23	6644.5
24	7040.0
25	7753.0
26	9085.0
27	9704.0
28	11992.5
29	14281.0
30	17195.0
31	20109.0
32	23218.0
33	26327.0
34	33056.0
35	39785.0
36	40652.0
37	41519.0
38	47738.5
39	53958.0
40	68901.5
41	83845.0
42	97614.0
43	111383.0
44	116050.5
45	120718.0
46	124098.0
47	127478.0
48	127205.5
49	126933.0
50	123819.0
51	120705.0
52	112922.5
53	105140.0
54	96413.0
55	87686.0
56	82118.5
57	76551.0
58	72759.5
59	68968.0
60	63187.0
61	57406.0
62	50810.5
63	44215.0
64	39324.0
65	34433.0
66	29679.5
67	24926.0
68	20707.5
69	16489.0
70	13771.5
71	11054.0
72	9574.5
73	8095.0
74	6524.5
75	3589.5
76	2225.0
77	1821.5
78	1418.0
79	986.0
80	554.0
81	452.5
82	351.0
83	242.5
84	134.0
85	97.0
86	60.0
87	47.5
88	35.0
89	30.5
90	26.0
91	16.0
92	6.0
93	3.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1535408.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.468810857812095
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.60493088211108	19.23369186245769
2	6.888838400155901	3.3712336768689526
3	2.814738755429884	2.0661993066230178
4	1.6037674428678743	1.5696912887780394
5	1.082639607792385	1.324545189512387
6	0.816775746686617	1.1991318755353848
7	0.65406054513137	1.1202858637863877
8	0.5098484906172874	0.9980309026444312
9	0.43024603134373524	0.9474847886946738
>10	4.620465359123225	25.422653786759973
>50	1.5251386369235496	26.945220430627426
>100	0.4424165391941649	13.96164240966937
>500	0.004533502808286971	0.8028027576247204
>1k	0.0013333831789079328	0.5193454562249167
>5k	2.666766357815866E-4	0.5180404041926892
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7939	0.5170612631951897	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2916	0.18991694715671664	No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	1546	0.10068984921271741	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06799495638944177	0.0
2	0.0	0.0	0.0	0.24234600835738773	0.0
3	0.0	0.0	0.0	0.3227806550441316	0.0
4	0.0	0.0	0.0	0.4264013213426008	0.0
5	0.0	0.0	0.0	0.6680309077456936	0.0
6	0.0	0.0	0.0	0.900086491668664	0.0
7	0.0	0.0	0.0	1.0464970874191095	0.0
8	0.0	0.0	0.0	1.4624777257901482	0.0
9	0.0	0.0	0.0	1.6680256974042078	0.0
10	0.0	0.0	0.0	2.063295228369267	0.0
11	0.0	0.0	0.0	2.413690693288038	0.0
12	0.0	0.0	0.0	2.7298281629377987	0.0
13	0.0	0.0	0.0	2.859435407396601	0.0
14	0.0	0.0	0.0	2.9160327417858967	0.0
15	0.0	0.0	0.0	2.9974443275012246	0.0
16	0.0	0.0	0.0	3.1613095672290363	0.0
17	0.0	0.0	0.0	3.3545481070829384	0.0
18	0.0	0.0	0.0	3.643396413200921	0.0
19	0.0	0.0	0.0	3.7638855600596064	0.0
20	0.0	0.0	0.0	3.8988334045413335	0.0
21	0.0	0.0	0.0	4.051561539343289	0.0
22	0.0	0.0	0.0	4.2116492814939095	0.0
23	0.0	0.0	0.0	4.392969165199087	0.0
24	0.0	0.0	0.0	4.5324760584808725	0.0
25	0.0	0.0	0.0	4.6411768077279785	0.0
26	0.0	0.0	0.0	4.749617039900795	0.0
27	0.0	0.0	0.0	4.883848462428228	0.0
28	0.0	0.0	0.0	5.0139116117670355	0.0
29	0.0	0.0	0.0	5.153483634317393	0.0
30	0.0	0.0	0.0	5.334347613142565	0.0
31	0.0	0.0	0.0	5.474505799110074	0.0
32	0.0	0.0	0.0	5.603787397226014	0.0
33	0.0	0.0	0.0	5.743619936850661	0.0
34	0.0	0.0	0.0	5.898888113126934	0.0
35	0.0	0.0	0.0	6.076039723643488	0.0
36	0.0	0.0	0.0	6.214569677896689	0.0
37	0.0	0.0	0.0	6.36918656148724	0.0
38	0.0	0.0	0.0	6.533963610975063	0.0
39	0.0	0.0	0.0	6.787642112064025	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	100	0.0	45.000004	4
TACGCTA	25	3.8914193E-5	45.000004	35
GCGATCG	25	3.8914193E-5	45.000004	9
ACGTTAT	20	7.034028E-4	45.0	28
AATATCG	20	7.034028E-4	45.0	1
CGTAAAT	65	0.0	44.999996	16
GTACGAG	150	0.0	43.5	1
CGTTTTT	6375	0.0	43.129414	1
CGGTCTA	205	0.0	42.80488	31
CGACGGT	220	0.0	40.909092	28
CGCATCG	105	0.0	40.714287	21
ACGGGTA	190	0.0	40.26316	5
ATAGGGC	710	0.0	39.929577	4
GTTAGCG	85	0.0	39.705883	1
ATTAGCG	120	0.0	39.375004	1
GTATACG	80	0.0	39.375	1
TAGTCGG	40	3.4592267E-7	39.375	2
ACGGTCT	230	0.0	39.130432	30
ATCGTAG	75	0.0	39.0	1
TGATTCG	75	0.0	39.0	15
>>END_MODULE