FastQCFastQC Report
Thu 26 May 2016
SRR1546373_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546373_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1514607
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76570.5055436822885409No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC37340.24653259888538742No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT15340.10128039814948696No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG351.2122655E-745.01
CACGGTA207.033997E-445.040
CAACGTA207.033997E-445.020
GTTTACG502.1827873E-1145.01
CGACCGT207.033997E-445.030
ATCCGTC207.033997E-445.026
TGCGCAA253.8913957E-545.015
CTTATCG351.2122655E-745.01
ACGGATA253.8913957E-545.025
ACTACGT253.8913957E-545.045
ACGCTAG207.033997E-445.01
CGTACAA207.033997E-445.039
CGACTAT207.033997E-445.027
ACGCGTA207.033997E-445.023
TCGATAG650.044.9999961
ACGTCGG302.1659616E-644.9999962
CGGTCTA1250.043.19999731
TGTTACG1200.043.1249961
CGTTTTT68850.043.1045761
GTTACGG2150.042.9069752
Produced by FastQC (version 0.11.5)