FastQCFastQC Report
Thu 26 May 2016
SRR1546371_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546371_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1112667
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC55400.4979027867277452No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45290.4070400218573931No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGT207.033188E-445.00000434
CTAGACG207.033188E-445.0000041
ACTATCC207.033188E-445.00000411
GTACCGT207.033188E-445.00000411
ACCGGTA207.033188E-445.00000419
TGCCCAA207.033188E-445.00000437
GATGTAC207.033188E-445.0000049
CAACGGC207.033188E-445.00000424
CCTAGCG207.033188E-445.0000041
GCGAACG207.033188E-445.0000041
CGTGACG406.8139343E-945.00000418
CGTTGGC207.033188E-445.00000413
CACGCTT800.045.00000428
TTCACGG800.045.0000042
CGTCTTA207.033188E-445.00000418
TAAGTTA207.033188E-445.00000415
ATAGACC406.8139343E-945.00000421
ACGGGTG800.045.0000045
GTTACGA800.045.00000437
AGACGAT207.033188E-445.00000415