##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546371_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1112667
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15867730417097	31.0	31.0	33.0	30.0	34.0
2	31.486521124469405	31.0	31.0	34.0	30.0	34.0
3	31.527911765155253	31.0	31.0	34.0	30.0	34.0
4	35.380568489943535	37.0	35.0	37.0	33.0	37.0
5	35.2983974540451	37.0	35.0	37.0	33.0	37.0
6	35.33625154695879	37.0	35.0	37.0	33.0	37.0
7	35.799493469294944	37.0	35.0	37.0	35.0	37.0
8	35.736736148371435	37.0	35.0	37.0	35.0	37.0
9	37.526055863973674	39.0	37.0	39.0	35.0	39.0
10	37.0208606887775	39.0	37.0	39.0	33.0	39.0
11	36.61846985665972	39.0	35.0	39.0	32.0	39.0
12	35.27395258419635	35.0	35.0	39.0	31.0	39.0
13	34.620780521036394	35.0	33.0	39.0	27.0	39.0
14	35.416993583884484	36.0	33.0	40.0	27.0	41.0
15	35.948804089633285	36.0	34.0	40.0	30.0	41.0
16	36.203712341608046	36.0	35.0	40.0	31.0	41.0
17	36.180629963861605	36.0	35.0	40.0	31.0	41.0
18	36.102285769237334	36.0	35.0	40.0	31.0	41.0
19	35.93994789096828	36.0	35.0	40.0	31.0	41.0
20	35.75402164349262	35.0	34.0	40.0	31.0	41.0
21	35.550427935761554	35.0	34.0	40.0	30.0	41.0
22	35.45115564674786	35.0	34.0	40.0	30.0	41.0
23	35.468652346119725	35.0	34.0	40.0	30.0	41.0
24	35.4063632695137	35.0	34.0	40.0	30.0	41.0
25	35.33668294287509	35.0	34.0	40.0	30.0	41.0
26	35.211674292488226	35.0	34.0	40.0	30.0	41.0
27	35.08745204090712	35.0	34.0	40.0	29.0	41.0
28	35.10555539078628	35.0	34.0	40.0	29.0	41.0
29	35.13170067953844	35.0	34.0	40.0	29.0	41.0
30	35.01454523231119	36.0	34.0	40.0	29.0	41.0
31	34.70519032199211	35.0	34.0	40.0	29.0	41.0
32	34.423506763479104	35.0	34.0	40.0	27.0	41.0
33	34.20112126988578	35.0	33.0	40.0	25.0	41.0
34	34.00535470181105	35.0	33.0	39.0	24.0	41.0
35	33.79968669871579	35.0	33.0	39.0	23.0	41.0
36	33.53561847345163	35.0	33.0	39.0	23.0	41.0
37	33.335351906725016	35.0	33.0	39.0	22.0	41.0
38	33.351721584265555	35.0	33.0	39.0	23.0	41.0
39	33.34620960269335	35.0	33.0	39.0	23.0	41.0
40	33.09281393265011	35.0	32.0	39.0	21.0	41.0
41	33.09884718428784	35.0	32.0	39.0	21.0	41.0
42	33.00880676788293	35.0	32.0	39.0	21.0	41.0
43	32.90329092172231	35.0	32.0	39.0	21.0	41.0
44	32.739965326553225	35.0	32.0	38.0	20.0	41.0
45	32.68802795445538	35.0	32.0	38.0	20.0	41.0
46	32.63391293172171	35.0	32.0	38.0	20.0	40.0
47	32.57596118155747	35.0	32.0	38.0	20.0	40.0
48	32.575344644893754	35.0	32.0	38.0	20.0	40.0
49	32.64682604948291	35.0	32.0	38.0	20.0	40.0
50	32.45211370517864	35.0	32.0	38.0	19.0	40.0
51	32.21830251099386	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	0.0
12	2.0
13	3.0
14	13.0
15	37.0
16	146.0
17	367.0
18	928.0
19	1660.0
20	2940.0
21	4689.0
22	6939.0
23	9980.0
24	13892.0
25	19228.0
26	24726.0
27	27783.0
28	29033.0
29	31432.0
30	36351.0
31	44358.0
32	54484.0
33	71913.0
34	133989.0
35	194555.0
36	65563.0
37	76590.0
38	106989.0
39	154052.0
40	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.64247074821128	23.625936600977653	28.9319266231496	14.799666027661464
2	30.859367627511197	25.17905177380115	31.671290691644487	12.290289907043167
3	28.0582600184961	23.690645988422414	34.426921981149796	13.824172011931694
4	24.394720073481103	28.01044697110636	32.91955275028378	14.675280205128757
5	21.53330691033346	32.091632087587755	31.81724630999212	14.557814692086671
6	19.512756287370795	40.507537295525076	28.816618089689012	11.163088327415121
7	85.69428229650022	4.9529643640010885	7.8555398874955396	1.49721345200316
8	88.47004539543278	2.8749841596811985	6.820099814230134	1.8348706306558926
9	84.96621181359741	4.12756017748347	8.554131649451273	2.3520963594678372
10	59.93239666495007	18.750533627761044	12.420607423424979	8.896462283863906
11	56.154896298712906	16.852301721898826	17.183218339359396	9.809583640028867
12	49.30558738598341	20.652270625443194	19.64909537175094	10.393046616822463
13	20.712935676172656	47.4070858576735	20.627734982703718	11.252243483450124
14	13.21662276314477	49.68018283996919	25.27953107263898	11.823663324247056
15	11.317492115790259	21.506614288012496	55.70148121585344	11.474412380343804
16	12.069109625791006	14.460121491874927	53.89060698304164	19.58016189929242
17	13.019528753885934	15.615004309465455	29.42982940987735	41.935637526771266
18	20.888100393019656	21.3800714858983	36.69714299067016	21.034685130411884
19	30.18935584501023	22.96194638647502	26.306073605130738	20.542624163384012
20	32.57785123491575	22.029951458972004	23.601850328984323	21.79034697712793
21	21.515332080487692	28.33444327907631	27.907181573642433	22.243043066793568
22	22.717488700572588	24.47983089280081	24.297655992313963	28.50502441431264
23	17.768928169883715	31.1822854456904	24.363084372952553	26.68570201147333
24	19.910089901111476	21.9074529935731	41.91712345203012	16.265333653285303
25	15.665154084735144	22.84142515235915	39.158616189749495	22.33480457315621
26	14.87650842525212	35.707358985213006	27.634323656583685	21.78180893295119
27	16.43079196201559	35.384351292884574	29.048133898102485	19.13672284699735
28	12.303052036233662	28.305413928875396	42.952114154549385	16.439419880341557
29	12.986275318671265	23.415001972737574	41.66502646344324	21.933696245147917
30	17.198856441325212	29.68668972837336	33.72176940630035	19.39268442400107
31	30.70271698540534	25.213743195403477	24.383755427275187	19.699784391916
32	31.776892816988372	27.049961938297802	25.549513016922404	15.623632227791424
33	28.701579178676102	27.978451773980893	23.978153391805453	19.341815655537552
34	18.468957918227105	27.567097792960517	29.280548448008254	24.683395840804124
35	18.79798717855387	25.50322783006955	31.474016934087196	24.224768057289378
36	31.96437029227972	22.314403141281264	27.313742566284432	18.407484000154582
37	19.63795097724656	32.34813290948684	29.26221412156557	18.75170199170102
38	19.893912554250285	32.89924119255806	22.985403539423746	24.22144271376791
39	20.060898723517457	31.299301587986346	27.63549202052366	21.004307667972537
40	23.11769828708859	25.682796380228766	24.990316060420593	26.20918927226205
41	15.43489651441087	23.696757430569974	27.362903725912606	33.50544232910655
42	22.660148993364594	25.223898974266334	23.299333942680065	28.816618089689012
43	23.154996059018558	26.274617652900645	24.593072320829144	25.977313967251657
44	19.346309362998994	31.62662323947776	27.036031445167332	21.991035952355915
45	17.022613234687466	39.22916739689413	21.35445735336808	22.393762015050324
46	23.031508977978138	32.98210515814704	25.146067961034163	18.840317902840653
47	22.505655330840224	27.312843824792143	26.181418160150344	24.00008268421729
48	23.87749434466916	22.80367800968304	30.296306082592544	23.022521563055253
49	21.045380154170115	21.259011006887054	32.50972662980029	25.185882209142534
50	18.29981476937844	32.21098495776364	27.37944056937071	22.109759703487207
51	17.73810133669822	35.57632247563737	22.078573373704803	24.607002813959614
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	609.0
1	813.0
2	1017.0
3	4598.5
4	8180.0
5	6309.0
6	4438.0
7	4967.0
8	5496.0
9	6193.0
10	6890.0
11	6834.5
12	6779.0
13	6612.0
14	6445.0
15	6089.0
16	5733.0
17	5469.0
18	5205.0
19	5136.0
20	5067.0
21	4751.0
22	4435.0
23	4427.5
24	4420.0
25	4337.0
26	4272.0
27	4290.0
28	5126.5
29	5963.0
30	7928.0
31	9893.0
32	11750.0
33	13607.0
34	16553.5
35	19500.0
36	21355.5
37	23211.0
38	32286.0
39	41361.0
40	61555.0
41	81749.0
42	104605.0
43	127461.0
44	129223.0
45	130985.0
46	121740.5
47	112496.0
48	105310.5
49	98125.0
50	91390.0
51	84655.0
52	78205.0
53	71755.0
54	62113.0
55	52471.0
56	46911.5
57	41352.0
58	37874.0
59	34396.0
60	32375.5
61	30355.0
62	26182.5
63	22010.0
64	18233.0
65	14456.0
66	11669.0
67	8882.0
68	7569.5
69	6257.0
70	5148.5
71	4040.0
72	3158.5
73	2277.0
74	1667.0
75	757.5
76	458.0
77	344.5
78	231.0
79	256.5
80	282.0
81	172.5
82	63.0
83	53.0
84	43.0
85	26.5
86	10.0
87	6.5
88	3.0
89	2.5
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1112667.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.609010697326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.83045560059335	15.413928343358279
2	7.030854330166797	2.616744868828295
3	2.159235054682992	1.2054368469191126
4	1.0163895016344235	0.7565601243425932
5	0.636759087861204	0.5924728338814346
6	0.46574081689775404	0.5200185506298993
7	0.35493546676953697	0.46234985285822977
8	0.2672784764401391	0.3979030421791635
9	0.21827927373422726	0.3655765205932307
>10	2.3521652551526184	10.15282113879714
>50	0.9666611661346202	13.175232339886389
>100	1.6963908159086452	52.966015124570866
>500	0.0038841232190238006	0.46521015819010053
>1k	4.8551540237797507E-4	0.40919339802737725
>5k	4.8551540237797507E-4	0.5005368569378825
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5540	0.4979027867277452	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4529	0.4070400218573931	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.987414922883486E-5	0.0	0.0	0.06336127520632857	0.0
2	8.987414922883486E-5	0.0	0.0	0.19143193785741824	0.0
3	8.987414922883486E-5	0.0	0.0	0.2673755939557837	0.0
4	8.987414922883486E-5	0.0	0.0	0.41458945039261524	0.0
5	8.987414922883486E-5	0.0	0.0	0.6707307756947946	0.0
6	8.987414922883486E-5	0.0	0.0	0.9517672403333612	0.0
7	1.797482984576697E-4	0.0	0.0	1.1358294979540149	0.0
8	1.797482984576697E-4	0.0	0.0	1.7601852126467308	0.0
9	1.797482984576697E-4	0.0	0.0	2.0325038848101005	0.0
10	1.797482984576697E-4	0.0	0.0	2.498141851964694	0.0
11	1.797482984576697E-4	0.0	0.0	2.815846969488625	0.0
12	1.797482984576697E-4	0.0	0.0	3.134720450952531	0.0
13	1.797482984576697E-4	0.0	0.0	3.2483213755777784	0.0
14	1.797482984576697E-4	0.0	0.0	3.2924495828491365	0.0
15	1.797482984576697E-4	0.0	0.0	3.366056511067552	0.0
16	1.797482984576697E-4	0.0	0.0	3.5279198538286836	0.0
17	1.797482984576697E-4	0.0	0.0	3.6954452679912317	0.0
18	1.797482984576697E-4	0.0	0.0	3.995714800564769	0.0
19	1.797482984576697E-4	0.0	0.0	4.090981398747334	0.0
20	1.797482984576697E-4	0.0	0.0	4.2215685375768315	0.0
21	1.797482984576697E-4	0.0	0.0	4.36356969335839	0.0
22	1.797482984576697E-4	0.0	0.0	4.479687094162045	0.0
23	1.797482984576697E-4	0.0	0.0	4.63436050498487	0.0
24	1.797482984576697E-4	0.0	0.0	4.733132194987359	0.0
25	2.696224476865046E-4	0.0	0.0	4.814468300039454	0.0
26	2.696224476865046E-4	0.0	0.0	4.891310697630108	0.0
27	2.696224476865046E-4	0.0	0.0	4.967883472773076	0.0
28	2.696224476865046E-4	0.0	0.0	5.0459841084529335	0.0
29	2.696224476865046E-4	0.0	0.0	5.130915179474182	0.0
30	2.696224476865046E-4	0.0	0.0	5.265546655018977	0.0
31	2.696224476865046E-4	0.0	0.0	5.385708392537929	0.0
32	2.696224476865046E-4	0.0	0.0	5.484569956689647	0.0
33	2.696224476865046E-4	0.0	0.0	5.578039071887636	0.0
34	2.696224476865046E-4	0.0	0.0	5.684809561171492	0.0
35	2.696224476865046E-4	0.0	0.0	5.842628567217326	0.0
36	2.696224476865046E-4	0.0	0.0	5.9521851551272755	0.0
37	2.696224476865046E-4	0.0	0.0	6.066864569543268	0.0
38	2.696224476865046E-4	0.0	0.0	6.16707424593342	0.0
39	2.696224476865046E-4	0.0	0.0	6.278068820231031	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGT	20	7.033188E-4	45.000004	34
CTAGACG	20	7.033188E-4	45.000004	1
ACTATCC	20	7.033188E-4	45.000004	11
GTACCGT	20	7.033188E-4	45.000004	11
ACCGGTA	20	7.033188E-4	45.000004	19
TGCCCAA	20	7.033188E-4	45.000004	37
GATGTAC	20	7.033188E-4	45.000004	9
CAACGGC	20	7.033188E-4	45.000004	24
CCTAGCG	20	7.033188E-4	45.000004	1
GCGAACG	20	7.033188E-4	45.000004	1
CGTGACG	40	6.8139343E-9	45.000004	18
CGTTGGC	20	7.033188E-4	45.000004	13
CACGCTT	80	0.0	45.000004	28
TTCACGG	80	0.0	45.000004	2
CGTCTTA	20	7.033188E-4	45.000004	18
TAAGTTA	20	7.033188E-4	45.000004	15
ATAGACC	40	6.8139343E-9	45.000004	21
ACGGGTG	80	0.0	45.000004	5
GTTACGA	80	0.0	45.000004	37
AGACGAT	20	7.033188E-4	45.000004	15
>>END_MODULE