##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546370_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1343438
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.127376923981604	31.0	31.0	33.0	30.0	34.0
2	31.468858257694066	31.0	31.0	34.0	30.0	34.0
3	31.54407869957527	31.0	31.0	34.0	30.0	34.0
4	35.40662762256241	37.0	35.0	37.0	33.0	37.0
5	35.30334410668747	37.0	35.0	37.0	33.0	37.0
6	35.34843736741107	37.0	35.0	37.0	33.0	37.0
7	35.75423428546758	37.0	35.0	37.0	35.0	37.0
8	35.669667673536104	37.0	35.0	37.0	35.0	37.0
9	37.443606627175946	39.0	37.0	39.0	35.0	39.0
10	36.93016722766514	39.0	37.0	39.0	32.0	39.0
11	36.61454492131382	39.0	35.0	39.0	32.0	39.0
12	35.73008356172745	37.0	35.0	39.0	31.0	39.0
13	35.3172561740847	37.0	35.0	39.0	30.0	39.0
14	36.18625571109348	38.0	35.0	40.0	30.0	41.0
15	36.547599517059965	38.0	35.0	40.0	31.0	41.0
16	36.68004403627112	38.0	35.0	40.0	32.0	41.0
17	36.61733105658765	38.0	35.0	40.0	32.0	41.0
18	36.54210987034757	38.0	35.0	40.0	31.0	41.0
19	36.412390448982386	37.0	35.0	40.0	31.0	41.0
20	36.20927575370058	37.0	35.0	40.0	31.0	41.0
21	36.06020374591161	37.0	34.0	40.0	31.0	41.0
22	35.93376992462622	37.0	34.0	40.0	30.0	41.0
23	35.92341812573412	37.0	34.0	40.0	30.0	41.0
24	35.88726908126761	37.0	34.0	40.0	30.0	41.0
25	35.78895118345618	36.0	34.0	40.0	30.0	41.0
26	35.65928386721233	36.0	34.0	40.0	30.0	41.0
27	35.54437421004914	36.0	34.0	40.0	30.0	41.0
28	35.51430211144839	36.0	34.0	40.0	29.0	41.0
29	35.47321126840241	36.0	34.0	40.0	29.0	41.0
30	35.32127273458098	36.0	34.0	40.0	29.0	41.0
31	35.08219359583397	36.0	34.0	40.0	28.0	41.0
32	34.849836762098434	35.0	34.0	40.0	27.0	41.0
33	34.66665004265176	36.0	33.0	40.0	26.0	41.0
34	34.4483169301449	35.0	34.0	40.0	25.0	41.0
35	34.254826050774206	35.0	33.0	40.0	24.0	41.0
36	34.00770709180476	35.0	33.0	40.0	23.0	41.0
37	33.80905482798611	35.0	33.0	40.0	23.0	41.0
38	33.774838139162355	35.0	33.0	40.0	23.0	41.0
39	33.76749503884809	35.0	33.0	40.0	23.0	41.0
40	33.512721837554096	35.0	32.0	40.0	22.0	41.0
41	33.46089659515363	35.0	32.0	40.0	22.0	41.0
42	33.34282936763736	35.0	32.0	39.0	22.0	41.0
43	33.28369749850756	35.0	32.0	39.0	22.0	41.0
44	33.044785096148836	35.0	32.0	39.0	21.0	41.0
45	33.01981260020931	35.0	32.0	39.0	21.0	41.0
46	32.973854394471495	35.0	32.0	39.0	21.0	41.0
47	32.93177429847898	35.0	32.0	39.0	21.0	41.0
48	32.86198544331781	35.0	32.0	39.0	20.0	41.0
49	32.89143376918027	35.0	32.0	39.0	20.0	40.0
50	32.72482243319007	35.0	32.0	38.0	20.0	40.0
51	32.51492811726332	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	4.0
12	5.0
13	8.0
14	20.0
15	55.0
16	162.0
17	431.0
18	966.0
19	1945.0
20	3277.0
21	5081.0
22	7576.0
23	10977.0
24	15367.0
25	21059.0
26	27072.0
27	31298.0
28	33132.0
29	36554.0
30	42575.0
31	50953.0
32	62387.0
33	81470.0
34	141204.0
35	192189.0
36	96020.0
37	117391.0
38	157728.0
39	206504.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.065445521118207	24.0787442368014	29.918314056919638	14.937496185160759
2	32.01197226816571	25.738887838515804	29.495146035767934	12.753993857550553
3	28.264720813316284	25.39127224330412	32.319839099385305	14.02416784399429
4	26.420646133278947	27.316630912628643	31.222505244008282	15.040217710084129
5	22.42857504402883	32.504514536584495	30.808492837034535	14.258417582352145
6	20.021392874103604	41.31288529876333	28.416867767623067	10.248854059510002
7	86.82663435156665	4.189549499120912	7.252660710803179	1.7311554385092576
8	88.85158823853426	2.8819342612014847	6.536289728294123	1.7301877719701244
9	84.7553813424959	4.234211031696289	8.27682408864421	2.7335835371636055
10	52.99686327169546	21.152371750687415	13.688610862577951	12.162154115039176
11	45.70229515615905	20.26435161131366	19.04070005463594	14.992653177891349
12	40.37238785861349	20.249836613226663	25.471737437827425	13.906038090332414
13	22.272631859453135	38.130378923329545	24.584312785554673	15.012676431662644
14	16.41482524686662	39.70849417688051	28.41530461398293	15.461375962269939
15	15.221617968227786	21.33005021444979	47.6428387465592	15.805493070763221
16	16.53682566668503	15.839659143183384	45.37887122442569	22.244643965705897
17	16.89240590187266	16.529382077922463	29.793931688697207	36.78428033150767
18	22.80410409710013	21.65399519739653	34.06215992103841	21.479740784464934
19	29.84856763021442	24.178860505657873	24.381177248224333	21.591394615903376
20	31.749288020734863	22.205490688814816	24.102265977291097	21.942955313159224
21	23.5579907669725	25.6223956743817	27.527731089934925	23.291882468710874
22	22.73205015787852	22.810654455211182	24.97510119558923	29.48219419132107
23	20.701588015226605	28.07282509501741	24.82176326708043	26.403823622675553
24	21.995953665148672	21.777409899079824	36.87494324263568	19.35169319313582
25	19.84319335912785	23.373985252761944	33.59797772580499	23.18484366230522
26	18.797592445650636	30.857546086979827	26.139055170391188	24.20580629697835
27	18.851632900066846	29.96528310201141	28.833262123000836	22.34982187492091
28	16.182957456912785	26.21847826248774	38.101795542481305	19.496768738118174
29	18.213642907227577	22.55593484775628	36.246629915187754	22.983792329828397
30	20.319508604044252	27.019855028665262	32.22805964994291	20.43257671734758
31	30.06621816563176	24.839776751885832	23.875310955920558	21.21869412656185
32	29.997364969578054	25.896319740844014	24.878557849338787	19.22775744023915
33	27.22686123215213	26.236417311405514	24.215110782931554	22.321610673510797
34	19.58385872664016	27.123172040689635	28.208893897597058	25.08407533507315
35	21.23231589399734	24.623317190670505	30.106264673174348	24.03810224215781
36	30.52146805435011	24.84446621280625	24.525880613768557	20.108185119075088
37	21.5885660521736	30.64130983342737	27.139473500079646	20.630650614319382
38	22.418526199199366	30.56263110020708	22.90280608409171	24.11603661650184
39	21.67989888629025	28.427660971328784	25.89200245936173	24.00043768301924
40	23.15298510240145	24.00192640077175	26.883860661973237	25.961227834853563
41	18.226297008123932	23.95272427905121	27.66744725100823	30.153531461816623
42	23.364904074471617	25.34638740306587	23.703364055505354	27.585344466957164
43	23.470677470787635	24.97912073352101	25.818459802387604	25.731741993303746
44	21.302062320702557	28.16840077472872	27.64362776696803	22.885909137600695
45	19.389655495824893	33.978568419234826	22.69758634190785	23.934189743032427
46	23.71445500276157	29.865464576705435	25.639069313209838	20.781011107323152
47	23.363043177280975	26.57651488196701	26.447889668149926	23.612552272602088
48	23.60004704348098	23.41954001598883	28.959505388413902	24.020907552116284
49	21.56779843952605	23.054655294847993	31.226375910164816	24.151170355461137
50	20.115926451388155	29.79899332905575	27.49349058162714	22.591589637928955
51	19.404468237462392	30.98297055762901	25.15419394121649	24.45836726369211
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	496.0
1	899.5
2	1303.0
3	6520.5
4	11738.0
5	8841.5
6	5945.0
7	6263.0
8	6581.0
9	6861.5
10	7142.0
11	7214.0
12	7286.0
13	6892.0
14	6498.0
15	6045.5
16	5593.0
17	5345.0
18	5097.0
19	4818.5
20	4540.0
21	4392.5
22	4245.0
23	4230.0
24	4215.0
25	4750.0
26	5287.0
27	5289.0
28	6974.5
29	8660.0
30	10011.0
31	11362.0
32	14662.0
33	17962.0
34	21697.0
35	25432.0
36	28133.5
37	30835.0
38	39120.0
39	47405.0
40	63156.5
41	78908.0
42	93570.0
43	108232.0
44	112892.0
45	117552.0
46	117419.5
47	117287.0
48	115266.5
49	113246.0
50	108825.5
51	104405.0
52	95047.5
53	85690.0
54	79808.5
55	73927.0
56	70596.5
57	67266.0
58	63686.0
59	60106.0
60	55081.5
61	50057.0
62	47450.5
63	44844.0
64	38284.0
65	31724.0
66	28804.0
67	25884.0
68	20902.5
69	15921.0
70	13289.0
71	10657.0
72	8802.5
73	6948.0
74	5495.5
75	2948.0
76	1853.0
77	1390.5
78	928.0
79	714.0
80	500.0
81	416.5
82	333.0
83	225.0
84	117.0
85	80.0
86	43.0
87	37.0
88	31.0
89	22.5
90	14.0
91	8.5
92	3.0
93	3.0
94	3.0
95	5.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1343438.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.143640157350724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.14439011275735	15.725426452636654
2	6.677981015108421	2.5568173106171064
3	2.1331385262226004	1.2250810905536074
4	1.0900570547938906	0.8347064003182313
5	0.6796253974558613	0.6505252025345737
6	0.48541323542735876	0.5575545783982035
7	0.40893784505272623	0.5479991266688288
8	0.31512581883119994	0.4826124223995991
9	0.26140283583342233	0.45037816427754573
>10	3.0418202346148515	13.55486058066801
>50	1.240488673520571	17.345219966715224
>100	1.5145909859458289	44.383996714384175
>500	0.004685509623962347	0.574140093339438
>1k	0.0019522956766509782	0.6525508416681804
>5k	3.9045913533019563E-4	0.45813105482065036
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6129	0.4562175552574812	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4098	0.3050382674898283	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.030220970376005442	0.0
2	0.0	0.0	0.0	0.12289365046991375	0.0
3	0.0	0.0	0.0	0.19308669250088206	0.0
4	0.0	0.0	0.0	0.32133972687984114	0.0
5	0.0	0.0	0.0	0.5631819257755103	0.0
6	0.0	0.0	0.0	0.8339796849575493	0.0
7	0.0	0.0	0.0	1.0015348680028404	0.0
8	0.0	0.0	0.0	1.4896854190517166	0.0
9	0.0	0.0	0.0	1.725051695723956	0.0
10	7.443588762562917E-5	0.0	0.0	2.0996875181437478	0.0
11	7.443588762562917E-5	0.0	0.0	2.365349201079618	0.0
12	7.443588762562917E-5	0.0	0.0	2.6253537565559406	0.0
13	7.443588762562917E-5	0.0	0.0	2.722864769345515	0.0
14	7.443588762562917E-5	0.0	0.0	2.755542124013166	0.0
15	7.443588762562917E-5	0.0	0.0	2.8100291937551267	0.0
16	7.443588762562917E-5	0.0	0.0	2.946916791098659	0.0
17	7.443588762562917E-5	0.0	0.0	3.1075494365947667	0.0
18	7.443588762562917E-5	0.0	0.0	3.388693784156768	0.0
19	7.443588762562917E-5	0.0	0.0	3.4777191057570205	0.0
20	7.443588762562917E-5	0.0	0.0	3.596444346519899	0.0
21	7.443588762562917E-5	0.0	0.0	3.711075613463368	0.0
22	7.443588762562917E-5	0.0	0.0	3.824888085642955	0.0
23	7.443588762562917E-5	0.0	0.0	3.9604358370092254	0.0
24	7.443588762562917E-5	0.0	0.0	4.055416029619528	0.0
25	7.443588762562917E-5	0.0	0.0	4.1294053019194035	0.0
26	7.443588762562917E-5	0.0	0.0	4.1993750362874955	0.0
27	7.443588762562917E-5	0.0	0.0	4.272247770272986	0.0
28	7.443588762562917E-5	0.0	0.0	4.352638528908666	0.0
29	7.443588762562917E-5	0.0	0.0	4.4440457989129385	0.0
30	7.443588762562917E-5	0.0	0.0	4.569693577225	0.0
31	7.443588762562917E-5	0.0	0.0	4.674127127563758	0.0
32	7.443588762562917E-5	0.0	0.0	4.769181756061687	0.0
33	7.443588762562917E-5	0.0	0.0	4.871828845097429	0.0
34	7.443588762562917E-5	0.0	0.0	4.977527805525822	0.0
35	7.443588762562917E-5	0.0	0.0	5.116797351273375	0.0
36	7.443588762562917E-5	0.0	0.0	5.219146696758615	0.0
37	7.443588762562917E-5	0.0	0.0	5.319486273277963	0.0
38	7.443588762562917E-5	0.0	0.0	5.426599515571243	0.0
39	7.443588762562917E-5	0.0	0.0	5.539369885324072	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGC	30	2.165776E-6	45.000004	11
TAGAACG	40	6.8157533E-9	45.000004	1
GCACGAC	20	7.033712E-4	45.000004	40
CGACATA	20	7.033712E-4	45.000004	9
CACGACA	20	7.033712E-4	45.000004	41
CCTCGTA	30	2.165776E-6	45.000004	17
ACGGGAT	240	0.0	45.000004	5
CCGTAGG	55	1.8189894E-12	45.000004	2
CGTAGTC	20	7.033712E-4	45.000004	15
TACGACA	20	7.033712E-4	45.000004	14
CGCTAAA	30	2.165776E-6	45.000004	44
ACTCCGA	20	7.033712E-4	45.000004	28
CAATCGA	20	7.033712E-4	45.000004	40
TCGACCT	20	7.033712E-4	45.000004	43
TATCGCC	20	7.033712E-4	45.000004	40
ATACCTC	20	7.033712E-4	45.000004	16
AATACCG	40	6.8157533E-9	45.000004	1
TAGTCCG	20	7.033712E-4	45.000004	1
TGGTCGA	40	6.8157533E-9	45.000004	13
AATCGAC	20	7.033712E-4	45.000004	41
>>END_MODULE