FastQCFastQC Report
Thu 26 May 2016
SRR1546369_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546369_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2979358
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121760.4086786482188445No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC60230.202157646043208No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC600.044.99999633
CGTTTTT122400.043.5845571
CTACGCG900.042.51
ACTACGG1650.042.2727282
GGTACGC1100.040.9090929
TACGCGG2150.040.8139532
CGACGGT3550.040.56337728
CGTAAGG4950.040.4545442
ACACGTA451.9301297E-840.032
AGGGCGA26650.039.427776
TATCTCG403.4607E-739.37530
TCGACAA3200.039.37519
CGACAGG3050.039.098362
CGAACGG1500.039.02
CGTTTAG750.039.030
TTGTACG1100.038.8636361
CTAAGCG1450.038.7931021
CGCATCC356.251639E-638.57142619
ATAGGGC12950.038.3976864
TAAGGGA45600.038.2894744
Produced by FastQC (version 0.11.5)