##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546369_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2979358
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.124711766763173	31.0	31.0	33.0	30.0	34.0
2	31.457698604867222	31.0	31.0	34.0	30.0	34.0
3	31.539323572393783	31.0	31.0	34.0	30.0	34.0
4	35.38627080062215	37.0	35.0	37.0	33.0	37.0
5	35.29997402124887	37.0	35.0	37.0	33.0	37.0
6	35.35085948046525	37.0	35.0	37.0	33.0	37.0
7	35.813059055004466	37.0	35.0	37.0	35.0	37.0
8	35.73574374076563	37.0	35.0	37.0	35.0	37.0
9	37.52619993971856	39.0	37.0	39.0	35.0	39.0
10	36.90880484990391	39.0	37.0	39.0	32.0	39.0
11	36.58711574775506	39.0	35.0	39.0	32.0	39.0
12	36.156892525168175	38.0	35.0	39.0	32.0	39.0
13	35.95207188931307	38.0	35.0	39.0	31.0	39.0
14	36.93116604315426	39.0	35.0	41.0	31.0	41.0
15	37.153411238260055	39.0	35.0	41.0	32.0	41.0
16	37.194995700415994	39.0	35.0	41.0	32.0	41.0
17	37.124480508888155	39.0	35.0	41.0	32.0	41.0
18	37.02870114971078	38.0	35.0	40.0	32.0	41.0
19	36.95839674184841	38.0	35.0	40.0	31.0	41.0
20	36.83169998368776	38.0	35.0	40.0	31.0	41.0
21	36.69365950651113	38.0	35.0	40.0	31.0	41.0
22	36.591810718953546	38.0	35.0	40.0	31.0	41.0
23	36.52486810916983	38.0	35.0	40.0	31.0	41.0
24	36.53029511727023	38.0	35.0	40.0	31.0	41.0
25	36.47058594502574	38.0	35.0	40.0	31.0	41.0
26	36.367104255346284	38.0	35.0	40.0	30.0	41.0
27	36.28073162070486	38.0	35.0	40.0	30.0	41.0
28	36.24274424221594	38.0	35.0	40.0	30.0	41.0
29	36.183907741197935	38.0	35.0	40.0	30.0	41.0
30	36.0278902367557	38.0	35.0	40.0	30.0	41.0
31	35.830270816733	38.0	34.0	40.0	29.0	41.0
32	35.66578571625162	38.0	34.0	40.0	29.0	41.0
33	35.459766164388434	38.0	34.0	40.0	27.0	41.0
34	35.259908007027015	38.0	34.0	40.0	26.0	41.0
35	35.08551875941058	38.0	34.0	40.0	25.0	41.0
36	34.90775697314656	38.0	34.0	40.0	25.0	41.0
37	34.733516415281414	38.0	33.0	40.0	24.0	41.0
38	34.65780547352819	37.0	33.0	40.0	24.0	41.0
39	34.619538168961235	37.0	33.0	40.0	24.0	41.0
40	34.42910318263196	37.0	33.0	40.0	23.0	41.0
41	34.32730306327739	37.0	33.0	40.0	23.0	41.0
42	34.2109800165002	37.0	33.0	40.0	23.0	41.0
43	34.167883147980206	37.0	33.0	40.0	23.0	41.0
44	34.00936812561633	37.0	33.0	40.0	23.0	41.0
45	33.975571918513985	37.0	33.0	40.0	23.0	41.0
46	33.886599730545974	36.0	33.0	40.0	23.0	41.0
47	33.8329207164765	36.0	33.0	40.0	23.0	41.0
48	33.762127948370086	36.0	33.0	40.0	22.0	41.0
49	33.756779480680066	36.0	33.0	40.0	22.0	41.0
50	33.60952158149507	36.0	32.0	40.0	22.0	41.0
51	33.42462033767006	36.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	2.0
12	8.0
13	6.0
14	31.0
15	85.0
16	268.0
17	718.0
18	1629.0
19	3208.0
20	5760.0
21	9068.0
22	13894.0
23	20322.0
24	29252.0
25	41337.0
26	52840.0
27	60184.0
28	64555.0
29	72315.0
30	84133.0
31	101679.0
32	123301.0
33	157646.0
34	244515.0
35	322619.0
36	227762.0
37	294187.0
38	425877.0
39	622068.0
40	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.88068838991487	23.192748236365016	29.26127038106867	13.665292992651437
2	31.056791429563013	25.32189820760043	30.854398833574216	12.766911529262345
3	28.687992513823446	24.965009240245717	33.328757403440605	13.018240842490227
4	24.92647744916858	28.288241963537107	32.31659303782896	14.468687549465354
5	22.166654695407534	32.41987032105575	31.41146515457357	14.002009828963152
6	19.94718325223085	41.72130371710953	27.96686400224478	10.364649028414846
7	88.72931685282533	3.4326522693815247	6.566918107860821	1.2711127699323141
8	90.64201079561435	2.39370360997235	5.681190377255771	1.2830952171575218
9	86.24650008491763	4.60431408377241	7.144693588350242	2.004492242959725
10	41.9834742921126	34.820723122229694	12.922213443298858	10.273589142358858
11	32.713020724599055	23.28508356498279	28.111693861563463	15.890201848854685
12	31.584186928861858	22.67595904889577	29.672499914411095	16.06735410783128
13	22.530927803909435	32.20700567034911	29.188368769379174	16.073697756362275
14	17.991963369289625	35.25078221549743	29.73090175802975	17.026352657183192
15	16.85406721850815	25.087183211953718	40.588173693795774	17.47057587574236
16	19.33711893636146	22.503069453217776	38.6824275565407	19.477384053880066
17	19.795304894544394	21.774489671936035	30.588804702221083	27.84140073129849
18	22.332965692608944	24.131574654673926	32.851574063942635	20.683885588774494
19	25.625755615807165	25.51858487633913	28.08511095343359	20.770548554420113
20	27.249058354182342	24.741404020597727	27.431715154741394	20.57782247047854
21	23.36946415972837	26.104449347812515	29.675923470761152	20.850163021697963
22	23.06298873784218	24.27637094971467	28.348926178055812	24.31171413438734
23	19.81440296869326	28.52691754398095	28.566456263396343	23.09222322392945
24	19.95127809413974	25.002500538706663	35.88484499009518	19.161376377058414
25	19.762009130826172	26.812252841048306	32.14460967765539	21.281128350470134
26	19.206889537947436	30.4459886995789	28.938818362882206	21.408303399591453
27	19.068571148549452	29.668908536671324	29.767285435318612	21.495234879460607
28	17.76661280718866	27.29373240812282	34.86120164142745	20.078453143261065
29	18.628140693397704	24.908755510415332	34.70734299134243	21.755760804844535
30	20.403959510740233	27.52807819671218	31.616475764241827	20.45148652830576
31	25.446421678764352	26.956747057587577	27.138933958255436	20.45789730539264
32	25.706981168426218	27.144505628393766	27.363512541963736	19.785000661216277
33	24.978938415591546	27.272117013128334	27.359082057275426	20.389862514004694
34	20.556676975375233	27.229523944420244	29.61060738588649	22.603191694318035
35	20.883391656860304	27.05455336350986	30.333246289972536	21.728808689657303
36	26.119351887218656	26.36816387960091	27.642163177436213	19.870321055744224
37	21.376585156936496	30.365266611128977	27.70224323495196	20.555904996982573
38	20.780718530636467	31.067733384172026	26.576295967117748	21.57525211807376
39	21.48825350964872	30.21953051630586	26.8259135021706	21.466302471874812
40	23.510031355748453	27.315112853171726	26.99031133552933	22.184544455550494
41	19.977961695103442	25.92105413313875	28.481941411538997	25.619042760218814
42	22.69146574530486	26.59445424148424	26.05051155316011	24.66356846005079
43	22.366798484774236	26.796477630415684	27.33441231298823	23.50231157182185
44	21.141500954232423	28.744044857986182	27.841971324023497	22.272482863757897
45	20.07281434456685	31.43093243577979	25.420879263250672	23.07537395640269
46	21.90136264255588	30.192779786786282	27.194449273971106	20.711408296686738
47	21.396857980813316	27.77793068171062	28.674566802646744	22.15064453482932
48	22.192834832202106	26.18735311432866	29.523440956071745	22.096371097397494
49	21.127202571829233	25.272122383412803	30.56010724458088	23.040567800177087
50	19.972188639297457	29.202902101727958	29.031355077167632	21.793554181806954
51	20.195693166111624	30.445820878189195	26.593245927478335	22.765240028220845
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	815.0
1	1658.5
2	2502.0
3	12892.5
4	23283.0
5	17994.5
6	12706.0
7	12835.5
8	12965.0
9	13268.5
10	13572.0
11	13528.0
12	13484.0
13	13343.0
14	13202.0
15	12786.5
16	12371.0
17	11779.0
18	11187.0
19	10945.5
20	10704.0
21	10653.5
22	10603.0
23	12328.0
24	14053.0
25	16122.5
26	21985.0
27	25778.0
28	30674.5
29	35571.0
30	45780.0
31	55989.0
32	65208.5
33	74428.0
34	87453.0
35	100478.0
36	104391.0
37	108304.0
38	126385.0
39	144466.0
40	172583.5
41	200701.0
42	225902.0
43	251103.0
44	253887.5
45	256672.0
46	250892.5
47	245113.0
48	246776.5
49	248440.0
50	238309.0
51	228178.0
52	210548.0
53	192918.0
54	170885.5
55	148853.0
56	138377.0
57	127901.0
58	117241.0
59	106581.0
60	97561.5
61	88542.0
62	73741.5
63	58941.0
64	49185.5
65	39430.0
66	33751.5
67	28073.0
68	22394.0
69	16715.0
70	14259.5
71	11804.0
72	8996.5
73	6189.0
74	5196.0
75	3260.5
76	2318.0
77	1699.0
78	1080.0
79	814.0
80	548.0
81	375.0
82	202.0
83	139.5
84	77.0
85	59.0
86	41.0
87	38.0
88	35.0
89	25.0
90	15.0
91	13.5
92	12.0
93	12.5
94	13.0
95	8.5
96	4.0
97	3.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2979358.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.051366310736164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.63612433361399	15.362283425577461
2	7.415209551848278	2.8253974688626258
3	2.4841709682799973	1.4198055328559511
4	1.192758067966585	0.908946834916694
5	0.783784550115341	0.7466083286471453
6	0.5559970754352943	0.635550237108948
7	0.424941794660783	0.5667005253576943
8	0.3297927650338572	0.5026402218632443
9	0.2898288609159682	0.49694722170601624
>10	3.342386184127552	14.868861580872661
>50	1.1550603359294862	16.032818197992892
>100	1.3814226076589033	43.81300259154276
>500	0.007457541362180945	0.9930798746530238
>1k	7.102420344934233E-4	0.21172759258280058
>5k	1.7756050862335584E-4	0.2037442546934347
>10k+	1.7756050862335584E-4	0.41188611076660486
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12176	0.4086786482188445	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6023	0.202157646043208	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05239383786708412	0.0
2	0.0	0.0	0.0	0.1628874408513512	0.0
3	0.0	0.0	0.0	0.23008312529075056	0.0
4	0.0	0.0	0.0	0.3296012093880628	0.0
5	0.0	0.0	0.0	0.5300806415341829	0.0
6	0.0	0.0	0.0	0.7421061852922677	0.0
7	0.0	0.0	0.0	0.8828412027020587	0.0
8	0.0	0.0	0.0	1.2924260864253305	0.0
9	0.0	0.0	0.0	1.4896162193331584	0.0
10	0.0	0.0	0.0	1.819519507222697	0.0
11	0.0	0.0	0.0	2.102466370271716	0.0
12	0.0	0.0	0.0	2.3723231649234497	0.0
13	0.0	0.0	0.0	2.477580740548803	0.0
14	0.0	0.0	0.0	2.5193347023083494	0.0
15	0.0	0.0	0.0	2.580858023775592	0.0
16	0.0	0.0	0.0	2.724647390478083	0.0
17	0.0	0.0	0.0	2.9094858691033436	0.0
18	0.0	0.0	0.0	3.1823970130477774	0.0
19	0.0	0.0	0.0	3.2970861507747644	0.0
20	0.0	0.0	0.0	3.4344647403903794	0.0
21	0.0	0.0	0.0	3.584698448457688	0.0
22	0.0	0.0	0.0	3.75543993034741	0.0
23	0.0	0.0	0.0	3.939539994857953	0.0
24	0.0	0.0	0.0	4.076280863192674	0.0
25	0.0	0.0	0.0	4.19342019320941	0.0
26	0.0	0.0	0.0	4.310693780337911	0.0
27	0.0	0.0	0.0	4.436358436951854	0.0
28	0.0	0.0	0.0	4.5678297136497195	0.0
29	0.0	0.0	0.0	4.705409688932985	0.0
30	0.0	0.0	0.0	4.885045704477274	0.0
31	0.0	0.0	0.0	5.04115316118439	0.0
32	0.0	0.0	0.0	5.193736368707621	0.0
33	0.0	0.0	0.0	5.347360068847047	0.0
34	0.0	0.0	0.0	5.508200088743951	0.0
35	0.0	0.0	0.0	5.69569014532661	0.0
36	0.0	0.0	0.0	5.862873813754507	0.0
37	0.0	0.0	0.0	6.035327073819259	0.0
38	0.0	0.0	0.0	6.212747847019392	0.0
39	0.0	0.0	0.0	6.416013114234677	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAC	60	0.0	44.999996	33
CGTTTTT	12240	0.0	43.584557	1
CTACGCG	90	0.0	42.5	1
ACTACGG	165	0.0	42.272728	2
GGTACGC	110	0.0	40.909092	9
TACGCGG	215	0.0	40.813953	2
CGACGGT	355	0.0	40.563377	28
CGTAAGG	495	0.0	40.454544	2
ACACGTA	45	1.9301297E-8	40.0	32
AGGGCGA	2665	0.0	39.42777	6
TATCTCG	40	3.4607E-7	39.375	30
TCGACAA	320	0.0	39.375	19
CGACAGG	305	0.0	39.09836	2
CGAACGG	150	0.0	39.0	2
CGTTTAG	75	0.0	39.0	30
TTGTACG	110	0.0	38.863636	1
CTAAGCG	145	0.0	38.793102	1
CGCATCC	35	6.251639E-6	38.571426	19
ATAGGGC	1295	0.0	38.397686	4
TAAGGGA	4560	0.0	38.289474	4
>>END_MODULE