FastQCFastQC Report
Thu 26 May 2016
SRR1546368_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546368_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1649806
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65360.39616779184946593No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24310.14735065819860033No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAT207.0341805E-445.022
TACGAAC253.8915477E-545.041
CGTACTC207.0341805E-445.030
ACCCGTA207.0341805E-445.034
TATCGCG406.8175723E-945.032
TAGTTCG302.1660799E-644.9999961
TGTCGCG302.1660799E-644.9999961
CGTTTTT59650.043.264881
CGTATGG1200.041.2499962
TACGACG603.6379788E-1241.2499961
TCGTAAA1450.040.34482615
GCGATCT1400.040.178579
CGAGGGA11600.040.150864
CCGCTCG850.039.70588319
AACGGGA5600.039.3754
CCGTATT403.459445E-739.37517
CATACCG403.459445E-739.3751
AATCGCG403.459445E-739.3751
CGCAGTA2400.039.37499637
TAGGGAC19400.039.201035
Produced by FastQC (version 0.11.5)