##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546368_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1649806
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2225916259245	31.0	31.0	33.0	30.0	34.0
2	31.57259702049817	31.0	31.0	34.0	30.0	34.0
3	31.65039707698966	31.0	31.0	34.0	30.0	34.0
4	35.487978586573206	37.0	35.0	37.0	33.0	37.0
5	35.411205923605564	37.0	35.0	37.0	33.0	37.0
6	35.4553789960759	37.0	35.0	37.0	33.0	37.0
7	35.87602299906777	37.0	35.0	37.0	35.0	37.0
8	35.79067357010461	37.0	35.0	37.0	35.0	37.0
9	37.57851711049663	39.0	37.0	39.0	35.0	39.0
10	37.02950589342019	39.0	37.0	39.0	33.0	39.0
11	36.715413812290656	39.0	35.0	39.0	32.0	39.0
12	36.25634710990262	38.0	35.0	39.0	32.0	39.0
13	36.066749666324405	38.0	35.0	39.0	32.0	39.0
14	37.066768456412454	39.0	35.0	41.0	32.0	41.0
15	37.27765749427508	39.0	35.0	41.0	32.0	41.0
16	37.326252904887	39.0	35.0	41.0	32.0	41.0
17	37.23672359053125	39.0	35.0	41.0	32.0	41.0
18	37.15398537767471	39.0	35.0	41.0	32.0	41.0
19	37.080091841101314	38.0	35.0	41.0	32.0	41.0
20	36.95444555299229	38.0	35.0	41.0	32.0	41.0
21	36.84377193439713	38.0	35.0	41.0	31.0	41.0
22	36.74183449447996	38.0	35.0	41.0	31.0	41.0
23	36.67901559334855	38.0	35.0	40.0	31.0	41.0
24	36.67328522262618	38.0	35.0	40.0	31.0	41.0
25	36.618194502868825	38.0	35.0	40.0	31.0	41.0
26	36.51945562084269	38.0	35.0	40.0	31.0	41.0
27	36.42380195004746	38.0	35.0	40.0	31.0	41.0
28	36.39826803878759	38.0	35.0	40.0	31.0	41.0
29	36.34656802072486	38.0	35.0	40.0	30.0	41.0
30	36.21785894826422	38.0	35.0	40.0	30.0	41.0
31	36.04736071998769	38.0	35.0	40.0	30.0	41.0
32	35.8929298353867	38.0	35.0	40.0	30.0	41.0
33	35.724130594748715	38.0	34.0	40.0	29.0	41.0
34	35.53787293778784	38.0	34.0	40.0	27.0	41.0
35	35.36555449549826	38.0	34.0	40.0	27.0	41.0
36	35.18550908409837	38.0	34.0	40.0	26.0	41.0
37	35.04038050534427	38.0	34.0	40.0	25.0	41.0
38	34.97286650672867	38.0	34.0	40.0	25.0	41.0
39	34.92843582821253	38.0	34.0	40.0	25.0	41.0
40	34.75322613689125	37.0	34.0	40.0	24.0	41.0
41	34.691960751748994	37.0	34.0	40.0	24.0	41.0
42	34.58350012062024	37.0	33.0	40.0	24.0	41.0
43	34.590610653616245	37.0	33.0	40.0	24.0	41.0
44	34.36612365332651	37.0	33.0	40.0	23.0	41.0
45	34.35645039477369	37.0	33.0	40.0	23.0	41.0
46	34.274948690936995	37.0	33.0	40.0	23.0	41.0
47	34.256620475377105	37.0	33.0	40.0	23.0	41.0
48	34.18180622448943	36.0	33.0	40.0	23.0	41.0
49	34.17737055144666	36.0	33.0	40.0	24.0	41.0
50	34.053222621326384	36.0	33.0	40.0	23.0	41.0
51	33.85933679475041	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	5.0
12	4.0
13	5.0
14	12.0
15	45.0
16	132.0
17	316.0
18	829.0
19	1591.0
20	2691.0
21	4455.0
22	6623.0
23	9910.0
24	14145.0
25	20273.0
26	26551.0
27	30094.0
28	32434.0
29	36563.0
30	43087.0
31	52741.0
32	65937.0
33	84622.0
34	136735.0
35	182712.0
36	124497.0
37	161800.0
38	239396.0
39	371541.0
40	60.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.156137145821994	23.269584423865595	29.016078254049265	13.55820017626315
2	32.1948762460556	25.136531204274924	30.156818438046656	12.51177411162282
3	29.412246045898733	24.93808362922671	32.75075978630215	12.898910538572414
4	25.008031247310292	29.148518068184988	31.383871800684442	14.459578883820278
5	22.75685747293924	33.02242809154531	30.427092639983126	13.793621795532324
6	19.67194930797924	42.00409017787546	27.970682613592146	10.353277900553156
7	89.29904485739536	3.1580682819676977	6.30334718142618	1.2395396792107678
8	90.64411209560397	2.509264725670776	5.592233268638858	1.254389910086398
9	86.22116782215606	4.422459367949929	7.049192450506301	2.30718035938771
10	43.433106680421815	33.46902605518467	12.54868754265653	10.54917972173698
11	33.01242691564948	22.958820612847813	27.989048409328127	16.039704062174582
12	31.37077935223899	22.562470981436604	30.283681838955612	15.783067827368793
13	22.004041687325664	32.57146597842413	29.239983367741416	16.18450896650879
14	17.955808137441615	35.45301690016887	29.551353310631672	17.039821651757844
15	16.358832493032512	25.36716438175155	41.73375536275174	16.54024776246419
16	18.95768350945505	22.425182112321085	39.46633725419837	19.15079712402549
17	19.26268906768432	22.68424287461677	30.365752094488684	27.687315963210217
18	22.196609783210874	24.39007980332233	33.027943891584826	20.385366521881966
19	25.239634235782876	26.19356457668356	28.025901227174586	20.540899960358974
20	28.379154882452845	24.587739406936333	27.53438889178485	19.49871681882597
21	23.82698329379333	26.28769685647888	29.679974493970807	20.20534535575698
22	22.96421518651284	24.06689028891882	28.281203971860936	24.68769055270741
23	20.333178567661893	28.72865052012176	27.597062927398735	23.341107984817608
24	20.526292182232336	25.530516921383484	35.25032640201333	18.692864494370852
25	19.648552617701718	26.626160894068757	32.38471674851468	21.34056973971485
26	20.10466685173893	31.132751365918175	27.81423997730642	20.94834180503647
27	19.010538208734847	30.05710974502457	30.44970135882643	20.482650687414157
28	17.75972447669605	27.940557859530152	34.99162931884112	19.308088344932678
29	18.38628299327315	24.77709500389743	34.847794225502874	21.98882777732655
30	20.124972269466834	27.92928380670212	31.571105936091882	20.374637987739163
31	25.01857794189135	27.170346089176544	27.289390388930578	20.521685580001527
32	25.466085103339424	27.461713680275135	27.730472552530415	19.341728663855022
33	24.498880474431537	27.494141735452533	27.63597659361161	20.371001196504317
34	20.49568252267236	27.90194725925351	28.997409392377044	22.60496082569708
35	21.067507331164997	27.381522433546735	30.00480056443	21.54616967085827
36	25.6091928384307	26.999538127513173	27.757990939540772	19.633278094515354
37	20.829903637154914	30.206945543900314	28.68361492199689	20.279535896947884
38	21.34784332218455	30.334475689868988	26.983293793330855	21.33438719461561
39	20.99652928889821	29.584751176805028	27.728957222849232	21.689762311447527
40	22.038469977682222	28.01747599414719	27.80993644101185	22.134117587158734
41	19.6346115846348	25.99887501924469	28.775686353425794	25.59082704269472
42	21.970946887088544	27.591062221861236	26.00626982808888	24.431721062961344
43	22.26043546938246	27.64058319584242	26.78254291716723	23.316438417607888
44	20.99986301419682	29.337085693711867	28.264474732180634	21.39857655991068
45	19.874397353385792	31.8521692853584	25.98032738394696	22.293105977308848
46	21.723826922680605	30.02819725470752	28.061966073586834	20.186009749025036
47	21.661031660692228	28.40873411782961	28.18676862612938	21.743465595348788
48	21.927911524142836	26.283756999307794	29.410912555779284	22.37741892077008
49	21.000469146069296	25.397349749000792	31.1512990012159	22.45088210371401
50	19.834998781674937	29.675125438991007	28.927340547918966	21.562535231415087
51	20.028597301743357	30.423092169624795	27.146222040652052	22.4020884879798
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	397.0
1	948.0
2	1499.0
3	7187.5
4	12876.0
5	9753.0
6	6630.0
7	6757.5
8	6885.0
9	7169.5
10	7454.0
11	7289.0
12	7124.0
13	6852.5
14	6581.0
15	6301.5
16	6022.0
17	5693.0
18	5364.0
19	5859.0
20	6354.0
21	6318.5
22	6283.0
23	7180.0
24	8077.0
25	10171.5
26	14102.0
27	15938.0
28	20236.5
29	24535.0
30	29585.5
31	34636.0
32	39181.5
33	43727.0
34	51158.5
35	58590.0
36	65022.5
37	71455.0
38	77460.5
39	83466.0
40	94826.5
41	106187.0
42	121619.5
43	137052.0
44	141045.5
45	145039.0
46	142443.0
47	139847.0
48	133975.5
49	128104.0
50	122841.0
51	117578.0
52	106570.5
53	95563.0
54	87251.5
55	78940.0
56	74515.5
57	70091.0
58	64253.5
59	58416.0
60	52468.5
61	46521.0
62	39831.0
63	33141.0
64	29175.0
65	25209.0
66	20051.0
67	14893.0
68	12318.5
69	9744.0
70	8327.5
71	6911.0
72	5524.0
73	4137.0
74	3738.0
75	2440.5
76	1542.0
77	1152.5
78	763.0
79	576.5
80	390.0
81	271.5
82	153.0
83	97.0
84	41.0
85	31.0
86	21.0
87	14.0
88	7.0
89	5.5
90	4.0
91	5.0
92	6.0
93	5.0
94	4.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1649806.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.765863994756522
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.20293741431703	15.457486249218466
2	6.519421369469165	2.5772399222687365
3	2.367876213423943	1.4040935757287016
4	1.3430810831104403	1.0618863209076497
5	0.9456925982330577	0.934621563876127
6	0.7821735423916781	0.9276201515526501
7	0.5948572516164352	0.8230507272221583
8	0.5215213379828968	0.8246655869546707
9	0.4481506031799513	0.7972275484460717
>10	5.553359268725844	25.575225345070336
>50	1.8645013277331979	26.772027150153765
>100	0.8548837633652174	22.146917626342784
>500	6.176905804572984E-4	0.07368244960511268
>1k	6.176905804572984E-4	0.22525951867677363
>5k	3.088452902286492E-4	0.39899626397598714
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6536	0.39616779184946593	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2431	0.14735065819860033	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.038428760714896176	0.0
2	0.0	0.0	0.0	0.13074264489279347	0.0
3	0.0	0.0	0.0	0.18644616397321867	0.0
4	0.0	0.0	0.0	0.2689407118170258	0.0
5	0.0	0.0	0.0	0.4449614075836795	0.0
6	0.0	0.0	0.0	0.631771250680383	0.0
7	0.0	0.0	0.0	0.7506943240599198	0.0
8	0.0	0.0	0.0	1.1040085925254242	0.0
9	0.0	0.0	0.0	1.2600875496876602	0.0
10	0.0	0.0	0.0	1.5332711846120088	0.0
11	0.0	0.0	0.0	1.7420230014922966	0.0
12	0.0	0.0	0.0	1.9643521723160178	0.0
13	0.0	0.0	0.0	2.047634691594042	0.0
14	0.0	0.0	0.0	2.078850483026489	0.0
15	0.0	0.0	0.0	2.1313415031827985	0.0
16	0.0	0.0	0.0	2.247233917199962	0.0
17	0.0	0.0	0.0	2.396342357828739	0.0
18	1.2122637449494061E-4	0.0	0.0	2.6273998276160953	0.0
19	1.2122637449494061E-4	0.0	0.0	2.7279571052596485	0.0
20	1.2122637449494061E-4	0.0	0.0	2.8353030598749185	0.0
21	1.2122637449494061E-4	0.0	0.0	2.955741462935642	0.0
22	1.2122637449494061E-4	0.0	0.0	3.086302268266693	0.0
23	1.2122637449494061E-4	0.0	0.0	3.239532405628298	0.0
24	1.2122637449494061E-4	0.0	0.0	3.355121753709224	0.0
25	1.2122637449494061E-4	0.0	0.0	3.451375495058207	0.0
26	1.2122637449494061E-4	0.0	0.0	3.5459320671642605	0.0
27	1.2122637449494061E-4	0.0	0.0	3.648610806361475	0.0
28	1.2122637449494061E-4	0.0	0.0	3.750865253247958	0.0
29	1.2122637449494061E-4	0.0	0.0	3.866878893639616	0.0
30	1.2122637449494061E-4	0.0	0.0	4.011986863910059	0.0
31	1.2122637449494061E-4	0.0	0.0	4.134849794460682	0.0
32	1.2122637449494061E-4	0.0	0.0	4.242620041386684	0.0
33	1.2122637449494061E-4	0.0	0.0	4.36324028400915	0.0
34	1.2122637449494061E-4	0.0	0.0	4.484466658504091	0.0
35	1.2122637449494061E-4	0.0	0.0	4.634605523316075	0.0
36	1.2122637449494061E-4	0.0	0.0	4.759589915420358	0.0
37	1.2122637449494061E-4	0.0	0.0	4.891787276807091	0.0
38	1.2122637449494061E-4	0.0	0.0	5.021802563452915	0.0
39	1.2122637449494061E-4	0.0	0.0	5.1652133644804294	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAT	20	7.0341805E-4	45.0	22
TACGAAC	25	3.8915477E-5	45.0	41
CGTACTC	20	7.0341805E-4	45.0	30
ACCCGTA	20	7.0341805E-4	45.0	34
TATCGCG	40	6.8175723E-9	45.0	32
TAGTTCG	30	2.1660799E-6	44.999996	1
TGTCGCG	30	2.1660799E-6	44.999996	1
CGTTTTT	5965	0.0	43.26488	1
CGTATGG	120	0.0	41.249996	2
TACGACG	60	3.6379788E-12	41.249996	1
TCGTAAA	145	0.0	40.344826	15
GCGATCT	140	0.0	40.17857	9
CGAGGGA	1160	0.0	40.15086	4
CCGCTCG	85	0.0	39.705883	19
AACGGGA	560	0.0	39.375	4
CCGTATT	40	3.459445E-7	39.375	17
CATACCG	40	3.459445E-7	39.375	1
AATCGCG	40	3.459445E-7	39.375	1
CGCAGTA	240	0.0	39.374996	37
TAGGGAC	1940	0.0	39.20103	5
>>END_MODULE