FastQCFastQC Report
Thu 26 May 2016
SRR1546367_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546367_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2739111
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104330.38089000409256873No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC65520.23920169719299436No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT27890.10182135736740863No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG27800.10149278360752814No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT4750.045.00000428
CGACGGA207.034998E-445.028
CGCGCAT207.034998E-445.031
CGTACCG207.034998E-445.038
GCGATAC1800.043.759
CGGTCTA5050.042.77227831
CGCATCG1900.042.6315821
CGTTTTT93900.042.5079881
CGTAAAT2300.042.0652216
ATGCGTA556.184564E-1140.90909211
TAAACGG2400.040.3125042
AATACGG2350.040.2127652
GATCGAC451.9299478E-840.09
TTGCGAG2600.039.807691
TATTACG1700.039.7058831
TCACGAC5400.039.58333225
AAATCGT2450.039.48979612
TCGTTAG403.4605546E-739.3751
ACTACGA403.4605546E-739.37529
AGGGCGA25350.038.9644976
Produced by FastQC (version 0.11.5)