##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546367_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2739111
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.166983010181042	31.0	31.0	33.0	30.0	34.0
2	31.49716605132103	31.0	31.0	34.0	30.0	34.0
3	31.589256879330556	31.0	31.0	34.0	30.0	34.0
4	35.436831147040046	37.0	35.0	37.0	33.0	37.0
5	35.35064405933166	37.0	35.0	37.0	33.0	37.0
6	35.39886956023323	37.0	35.0	37.0	33.0	37.0
7	35.820464011863706	37.0	35.0	37.0	35.0	37.0
8	35.73101674229339	37.0	35.0	37.0	35.0	37.0
9	37.52025565959174	39.0	37.0	39.0	35.0	39.0
10	36.941577760083476	39.0	37.0	39.0	32.0	39.0
11	36.67193443420146	39.0	35.0	39.0	32.0	39.0
12	36.08463841005348	37.0	35.0	39.0	32.0	39.0
13	35.8409067759576	38.0	35.0	39.0	31.0	39.0
14	36.76106225706078	39.0	35.0	41.0	31.0	41.0
15	37.00261252647301	39.0	35.0	41.0	32.0	41.0
16	37.06749343126292	39.0	35.0	41.0	32.0	41.0
17	37.013620477592916	38.0	35.0	41.0	32.0	41.0
18	36.93455723408069	38.0	35.0	40.0	32.0	41.0
19	36.851533581516044	38.0	35.0	40.0	31.0	41.0
20	36.72442810824388	38.0	35.0	40.0	31.0	41.0
21	36.59255393446998	38.0	35.0	40.0	31.0	41.0
22	36.495501277604305	38.0	35.0	40.0	31.0	41.0
23	36.41855222369593	38.0	35.0	40.0	31.0	41.0
24	36.41903011597559	38.0	35.0	40.0	31.0	41.0
25	36.361065688831154	38.0	35.0	40.0	31.0	41.0
26	36.27868640591783	38.0	35.0	40.0	30.0	41.0
27	36.16826773358218	38.0	35.0	40.0	30.0	41.0
28	36.11393222107465	38.0	35.0	40.0	30.0	41.0
29	36.040553668690315	38.0	35.0	40.0	30.0	41.0
30	35.913130939198886	38.0	35.0	40.0	30.0	41.0
31	35.73648347949389	38.0	34.0	40.0	29.0	41.0
32	35.57051503206697	38.0	34.0	40.0	29.0	41.0
33	35.39407019284724	38.0	34.0	40.0	27.0	41.0
34	35.19902041209721	38.0	34.0	40.0	27.0	41.0
35	35.015543364252125	37.0	34.0	40.0	26.0	41.0
36	34.82996198401598	37.0	34.0	40.0	25.0	41.0
37	34.64630568093079	37.0	33.0	40.0	24.0	41.0
38	34.56428344816986	37.0	33.0	40.0	24.0	41.0
39	34.51455928584128	37.0	33.0	40.0	24.0	41.0
40	34.30952414852848	37.0	33.0	40.0	23.0	41.0
41	34.19758673525826	36.0	33.0	40.0	23.0	41.0
42	34.08194666079615	36.0	33.0	40.0	23.0	41.0
43	34.057787727478	36.0	33.0	40.0	23.0	41.0
44	33.88286783558607	36.0	33.0	40.0	23.0	41.0
45	33.8509786569438	36.0	33.0	40.0	23.0	41.0
46	33.765794449366965	35.0	33.0	40.0	23.0	41.0
47	33.69475716756276	35.0	33.0	40.0	23.0	41.0
48	33.61338660609227	35.0	33.0	40.0	23.0	41.0
49	33.59770232020535	35.0	32.0	39.0	23.0	41.0
50	33.454636194006014	35.0	32.0	39.0	22.0	41.0
51	33.24613898450994	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	1.0
11	3.0
12	5.0
13	14.0
14	32.0
15	113.0
16	281.0
17	713.0
18	1545.0
19	3005.0
20	5291.0
21	8425.0
22	12533.0
23	18265.0
24	25835.0
25	35909.0
26	47005.0
27	54473.0
28	58788.0
29	66569.0
30	78971.0
31	95453.0
32	118507.0
33	150697.0
34	245113.0
35	321317.0
36	212392.0
37	272231.0
38	382022.0
39	523538.0
40	62.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.049583605775744	23.46907445517907	28.713184679262728	12.76815725978246
2	31.67969461624593	25.33559246047349	30.23791295789035	12.746799965390231
3	29.863375379822138	24.86627960677753	31.78315884241274	13.487186170987595
4	25.854483443715864	27.80902270846271	31.28160925205295	15.054884595768481
5	22.665163989338147	32.89669531464771	29.698796434317558	14.739344261696589
6	19.84746145738526	42.06916039547138	27.749258792360003	10.334119354783358
7	88.57932372948741	3.7542472721988998	6.219864766342073	1.446564231971614
8	89.69001256247009	2.926825528428749	5.698308684825113	1.6848532242760517
9	85.71018115001546	4.502227182469057	7.31492809163265	2.472663575882832
10	44.70833785122253	28.57631545417473	14.056823546033732	12.658523148569007
11	37.54280129574888	24.011732273719467	22.886148096955544	15.559318333576114
12	34.63079809471029	22.456994258356087	26.866928722494272	16.04527892443935
13	24.60236186120241	31.973403049383542	26.682963925156738	16.741271164257306
14	19.589458039487994	35.52137901676858	27.986525555189257	16.90263738855417
15	18.04581851556947	24.692573612387378	39.83347151685346	17.428136355189693
16	20.031681812091588	20.564664958813278	38.85611061399118	20.54754261510395
17	20.95376930690286	19.569926154872878	28.93044495093481	30.545859587289453
18	23.26572380600859	23.238123610178633	32.38287897058571	21.113273613227065
19	27.304004839526403	25.32602731324141	25.65091374537213	21.719054101860056
20	28.57945515899137	24.74299143043126	24.957075489091167	21.720477921486207
21	24.67899256364565	26.425070031846097	28.156580730025183	20.739356674483073
22	24.793153691106347	23.667679038929055	26.58902833802646	24.950138931938138
23	21.364632539535638	28.524181750940357	25.57150842006768	24.539677289456325
24	21.77834341142071	25.87631534465014	33.17390204340021	19.17143920052893
25	21.106264039682948	25.33018924753323	31.107465159316288	22.45608155346753
26	19.930152520288516	29.46565509758458	27.032237831909693	23.571954550217207
27	20.397712980598453	29.36693693683827	28.930079868979387	21.305270213583896
28	18.49362804209103	27.503704669142653	33.491669377400186	20.510997911366132
29	19.223645920154386	25.73641593933214	32.891365118098534	22.148573022414936
30	21.9478509633235	27.219597891432656	30.48222580245927	20.350325342784576
31	27.018291701212547	25.86101841071793	25.605461041921995	21.515228846147526
32	26.701656121274386	26.531345389069667	25.958531801011347	20.8084666886446
33	25.67274564630641	27.217297875113495	26.338764657584157	20.771191820995934
34	21.204726643060468	26.630355615380317	28.558572471141186	23.606345270418032
35	21.98056230652938	25.953311129048807	29.052309307654927	23.013817256766885
36	28.316194560936015	25.28955562589468	25.870254984190126	20.523994828979184
37	22.551404452028414	29.10670651901292	27.30363975757098	21.038249271387688
38	22.168433480789933	29.84030220023942	24.795453707425512	23.195810611545134
39	22.253935674749947	28.0231797835137	26.630574664553574	23.092309877182778
40	23.932472981197183	25.66150112207939	26.08558032149847	24.320445575224955
41	20.848443162763395	24.33577901735271	27.505238013355427	27.310539806528467
42	22.772753641601234	26.327118543206176	25.236691758749462	25.66343605644313
43	23.308474902988596	26.064405568083952	26.086675567364743	24.540443961562712
44	21.737198675044567	27.638931025431248	27.3562480673474	23.26762223217679
45	20.502381977218157	30.66363502610884	25.25863318427037	23.57534981240264
46	23.352394262225957	28.02569884900612	27.325763724069596	21.29614316469833
47	22.848617671938083	27.43488672054546	27.27388557820402	22.44261002931243
48	23.28770173972504	26.02523227426709	28.23627812089397	22.4507878651139
49	22.394857309543134	25.41912321187422	29.095863584936865	23.090155893645786
50	21.476639683459343	28.248398841814005	27.873313640812658	22.401647833913994
51	21.611720006965765	29.688793188738977	25.657485220569743	23.042001583725522
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	820.0
1	1669.5
2	2519.0
3	10915.0
4	19311.0
5	14513.5
6	9716.0
7	10144.5
8	10573.0
9	10866.0
10	11159.0
11	11101.0
12	11043.0
13	10822.0
14	10601.0
15	10218.5
16	9836.0
17	9481.0
18	9126.0
19	8919.5
20	8713.0
21	9050.0
22	9387.0
23	10493.0
24	11599.0
25	12504.0
26	15677.0
27	17945.0
28	20757.0
29	23569.0
30	31050.0
31	38531.0
32	44818.0
33	51105.0
34	60511.5
35	69918.0
36	78662.5
37	87407.0
38	96251.0
39	105095.0
40	133167.0
41	161239.0
42	182972.5
43	204706.0
44	217311.0
45	229916.0
46	227556.5
47	225197.0
48	221686.0
49	218175.0
50	216026.5
51	213878.0
52	199376.5
53	184875.0
54	169598.0
55	154321.0
56	145632.0
57	136943.0
58	127882.5
59	118822.0
60	110225.0
61	101628.0
62	91110.0
63	80592.0
64	69809.5
65	59027.0
66	52459.5
67	45892.0
68	37414.5
69	28937.0
70	23749.0
71	18561.0
72	14362.5
73	10164.0
74	8803.0
75	5583.0
76	3724.0
77	3056.0
78	2388.0
79	1587.0
80	786.0
81	522.0
82	258.0
83	190.0
84	122.0
85	91.5
86	61.0
87	46.5
88	32.0
89	29.5
90	27.0
91	16.5
92	6.0
93	8.0
94	10.0
95	5.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2739111.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.354387838297257
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.37101774363067	14.751608306252677
2	7.205127802544148	2.644914202247875
3	2.318749080905438	1.2767765979190113
4	1.2308994820422081	0.9036962593344757
5	0.7805029126137446	0.7162826583516649
6	0.5783228575721366	0.6368857214178805
7	0.4705886157065987	0.6046156175476436
8	0.3617200325637553	0.5311319813245342
9	0.3099015590324069	0.5119248065556392
>10	3.7965650919503604	16.130640944320042
>50	1.1689696715131095	15.525375271827649
>100	1.3956246133092798	43.178515189868335
>500	0.009007902462127394	1.08150830755953
>1k	0.0026022829335034694	0.8820781168599402
>5k	2.0017561026949764E-4	0.24072708354158312
>10k+	2.0017561026949764E-4	0.38331893507163256
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10433	0.38089000409256873	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6552	0.23920169719299436	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2789	0.10182135736740863	No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	2780	0.10149278360752814	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.047314621422790096	0.0
2	0.0	0.0	0.0	0.1645789455045816	0.0
3	0.0	0.0	0.0	0.23109687778260904	0.0
4	0.0	0.0	0.0	0.3252515140861396	0.0
5	0.0	0.0	0.0	0.5251338846801024	0.0
6	0.0	0.0	0.0	0.7247972061008116	0.0
7	0.0	0.0	0.0	0.8473917267317754	0.0
8	0.0	0.0	0.0	1.203967272593188	0.0
9	0.0	0.0	0.0	1.3725986278029623	0.0
10	0.0	0.0	0.0	1.6706515362101062	0.0
11	0.0	0.0	0.0	1.9035008073787445	0.0
12	0.0	0.0	0.0	2.1309468656071258	0.0
13	0.0	0.0	0.0	2.216996682500271	0.0
14	0.0	0.0	0.0	2.2538699599979704	0.0
15	0.0	0.0	0.0	2.310275122110787	0.0
16	0.0	0.0	0.0	2.437287134402366	0.0
17	0.0	0.0	0.0	2.5913882277863145	0.0
18	0.0	0.0	0.0	2.8288375315932797	0.0
19	0.0	0.0	0.0	2.9271175939930876	0.0
20	0.0	0.0	0.0	3.049018458908748	0.0
21	0.0	0.0	0.0	3.175555864658278	0.0
22	0.0	0.0	0.0	3.3102711062092776	0.0
23	0.0	0.0	0.0	3.463897593051176	0.0
24	0.0	0.0	0.0	3.5812714417195943	0.0
25	0.0	0.0	0.0	3.678565782839761	0.0
26	0.0	0.0	0.0	3.778488714038971	0.0
27	0.0	0.0	0.0	3.8802735632108374	0.0
28	0.0	0.0	0.0	3.987242576149707	0.0
29	3.650819554227631E-5	0.0	0.0	4.10501801496909	0.0
30	3.650819554227631E-5	0.0	0.0	4.25192699383121	0.0
31	3.650819554227631E-5	0.0	0.0	4.378172334016401	0.0
32	3.650819554227631E-5	0.0	0.0	4.498722395696998	0.0
33	3.650819554227631E-5	0.0	0.0	4.628070932503283	0.0
34	3.650819554227631E-5	0.0	0.0	4.765597305111037	0.0
35	3.650819554227631E-5	0.0	0.0	4.915499956007625	0.0
36	3.650819554227631E-5	0.0	0.0	5.042219902734866	0.0
37	3.650819554227631E-5	0.0	0.0	5.179381193387197	0.0
38	3.650819554227631E-5	0.0	0.0	5.323333008410393	0.0
39	3.650819554227631E-5	0.0	0.0	5.493826281592823	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	475	0.0	45.000004	28
CGACGGA	20	7.034998E-4	45.0	28
CGCGCAT	20	7.034998E-4	45.0	31
CGTACCG	20	7.034998E-4	45.0	38
GCGATAC	180	0.0	43.75	9
CGGTCTA	505	0.0	42.772278	31
CGCATCG	190	0.0	42.63158	21
CGTTTTT	9390	0.0	42.507988	1
CGTAAAT	230	0.0	42.06522	16
ATGCGTA	55	6.184564E-11	40.909092	11
TAAACGG	240	0.0	40.312504	2
AATACGG	235	0.0	40.212765	2
GATCGAC	45	1.9299478E-8	40.0	9
TTGCGAG	260	0.0	39.80769	1
TATTACG	170	0.0	39.705883	1
TCACGAC	540	0.0	39.583332	25
AAATCGT	245	0.0	39.489796	12
TCGTTAG	40	3.4605546E-7	39.375	1
ACTACGA	40	3.4605546E-7	39.375	29
AGGGCGA	2535	0.0	38.964497	6
>>END_MODULE