FastQCFastQC Report
Thu 26 May 2016
SRR1546366_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546366_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1778100
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89100.5010966762274338No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC37500.21089927450649568No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG19960.11225465384399078No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG18090.10173781002193352No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC700.045.00000413
TAATACG207.03433E-445.01
ACGTTAG453.8562575E-1045.01
ATAGACG406.8193913E-945.01
TATGCGT750.045.020
CGGATTA253.8916703E-545.020
ACGTGTA453.8562575E-1045.021
ACTACCG253.8916703E-545.023
CGTACGT207.03433E-445.021
TATCGCC207.03433E-445.06
TAGTCGA207.03433E-445.012
ATTTCGC253.8916703E-545.042
TATATCG302.1661745E-644.99999621
TAGCACG302.1661745E-644.9999961
CATCGAA302.1661745E-644.99999629
TAATGCG302.1661745E-644.9999961
CGTTTTT73200.043.1864781
CGTTGAT2150.042.90697525
CTAACGG1050.042.8571432
GCGTAAG1000.042.751