##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546366_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1778100
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.216582306956862	31.0	31.0	33.0	30.0	34.0
2	31.55615544682526	31.0	31.0	34.0	30.0	34.0
3	31.632438558011362	31.0	31.0	34.0	30.0	34.0
4	35.48408975873123	37.0	35.0	37.0	33.0	37.0
5	35.39859175524436	37.0	35.0	37.0	33.0	37.0
6	35.44466902873854	37.0	35.0	37.0	33.0	37.0
7	35.84511669759856	37.0	35.0	37.0	35.0	37.0
8	35.75874810190653	37.0	35.0	37.0	35.0	37.0
9	37.522503233788875	39.0	37.0	39.0	35.0	39.0
10	37.03386648669929	39.0	37.0	39.0	33.0	39.0
11	36.75033012766436	39.0	35.0	39.0	32.0	39.0
12	36.04681232776559	37.0	35.0	39.0	32.0	39.0
13	35.72866711658512	37.0	35.0	39.0	31.0	39.0
14	36.658505708340364	38.0	35.0	41.0	31.0	41.0
15	36.948095157752654	38.0	35.0	41.0	32.0	41.0
16	37.02810471851977	38.0	35.0	41.0	32.0	41.0
17	36.949429728361736	38.0	35.0	41.0	32.0	41.0
18	36.8908407851077	38.0	35.0	40.0	32.0	41.0
19	36.773669647376416	38.0	35.0	40.0	31.0	41.0
20	36.631794612226535	38.0	35.0	40.0	31.0	41.0
21	36.47191890219898	38.0	35.0	40.0	31.0	41.0
22	36.37182610651819	38.0	35.0	40.0	31.0	41.0
23	36.318489398796466	38.0	35.0	40.0	31.0	41.0
24	36.29587874697711	38.0	35.0	40.0	31.0	41.0
25	36.24233338957314	38.0	35.0	40.0	31.0	41.0
26	36.1110972386255	38.0	35.0	40.0	31.0	41.0
27	36.029538833586415	37.0	35.0	40.0	30.0	41.0
28	35.99644283223666	37.0	35.0	40.0	30.0	41.0
29	35.94971486418087	37.0	35.0	40.0	30.0	41.0
30	35.81141555593049	37.0	35.0	40.0	30.0	41.0
31	35.60442213598785	37.0	34.0	40.0	29.0	41.0
32	35.411334008211014	37.0	34.0	40.0	29.0	41.0
33	35.22056745964794	37.0	34.0	40.0	27.0	41.0
34	35.015136943928916	37.0	34.0	40.0	27.0	41.0
35	34.818790844159494	37.0	34.0	40.0	26.0	41.0
36	34.62885045835442	36.0	33.0	40.0	24.0	41.0
37	34.4768365108824	36.0	33.0	40.0	24.0	41.0
38	34.388196951802485	36.0	33.0	40.0	24.0	41.0
39	34.36150835161127	36.0	33.0	40.0	24.0	41.0
40	34.09100894212924	36.0	33.0	40.0	23.0	41.0
41	34.01321298014735	36.0	33.0	40.0	23.0	41.0
42	33.89559698554637	36.0	33.0	40.0	23.0	41.0
43	33.863951408807154	35.0	33.0	40.0	23.0	41.0
44	33.671007817333106	35.0	33.0	40.0	23.0	41.0
45	33.615724087509136	35.0	33.0	40.0	23.0	41.0
46	33.51822675889995	35.0	33.0	39.0	23.0	41.0
47	33.44834261290141	35.0	32.0	39.0	23.0	41.0
48	33.35022046004162	35.0	32.0	39.0	22.0	41.0
49	33.35330521343007	35.0	32.0	39.0	22.0	41.0
50	33.202100556774084	35.0	32.0	39.0	22.0	41.0
51	32.99397615432203	35.0	32.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	1.0
12	6.0
13	14.0
14	24.0
15	62.0
16	153.0
17	420.0
18	1009.0
19	1903.0
20	3307.0
21	5200.0
22	7923.0
23	11953.0
24	16712.0
25	23880.0
26	30936.0
27	35730.0
28	39165.0
29	43965.0
30	51645.0
31	62392.0
32	77899.0
33	101614.0
34	173006.0
35	234229.0
36	133952.0
37	168654.0
38	234759.0
39	317507.0
40	76.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.89691243462122	23.58478150835161	29.39694055452449	14.121365502502673
2	31.827624992970023	26.122490298633373	28.862043754569484	13.187840953827118
3	29.39356616613239	24.908216635734775	32.65704965974917	13.041167538383668
4	26.874191552781056	27.35307350542714	31.06180754738204	14.710927394409765
5	22.82914346774647	31.695067768966872	30.331702378943813	15.14408638434284
6	20.07671109611383	42.027669984815255	27.612676452393004	10.282942466677914
7	86.70187278555763	4.255047522636522	7.333164613913729	1.709915077892132
8	88.20105730836286	3.095157752657331	6.706540689500027	1.9972442494797817
9	83.71902592655081	4.625161689443788	8.705078454530115	2.9507339294752826
10	48.74911422304707	24.39958382543164	14.027613745008718	12.823688206512571
11	41.412406501321634	21.37826893875485	21.98892075811259	15.220403801810923
12	38.24756762836736	20.433496428772287	26.133682020133854	15.185253922726504
13	22.88943254035206	34.28721669197458	26.578932568471963	16.244418199201395
14	17.66739778415162	37.68983746695911	28.42539789663124	16.217366852258028
15	16.60873966593555	22.77172262527417	43.760924582419435	16.858613126370845
16	17.76064338338676	18.01181035937236	42.98262189978067	21.24492435746021
17	18.033012766436084	18.30920645632979	29.27411281705191	34.383667960182215
18	22.571396434396267	23.37270119790788	32.18120465665598	21.87469771103987
19	28.01495978853833	24.532141049434788	25.426916371407682	22.0259827906192
20	30.41859288004049	22.626005286541815	24.70125414768573	22.25414768573196
21	23.855801136044093	26.47708227883696	27.801979641190034	21.865136943928913
22	23.682976210561833	23.500534278162082	25.50992632585344	27.306563185422643
23	21.582363196670602	28.487992801304763	24.510657443338395	25.418986558686242
24	21.424048141274394	23.514650469602387	35.06461953770879	19.99668185141443
25	21.44873741634329	23.535627917439964	31.56689724987346	23.44873741634329
26	19.181260896462515	29.529554018334174	27.0768235757269	24.212361509476406
27	19.79174399640065	29.007817333108378	28.98453405320286	22.215904617288114
28	17.964287722850234	26.63815308475339	35.83988527079467	19.557673921601708
29	18.96158821213655	23.786120015747144	34.69506776896687	22.55722400314943
30	20.75085765704966	26.969405545244925	31.631910466227996	20.64782633147742
31	29.372251279455597	24.673078004611664	24.73134244418199	21.223328271750745
32	28.872954277037287	25.8529329059108	25.569990439232885	19.70412237781902
33	26.83398009110849	26.122771497666047	25.245149316686348	21.798099094539115
34	21.363140430796918	26.58506270738429	28.422023508239132	23.629773353579665
35	21.260390304257353	23.91507789213205	30.86406838760475	23.960463416005847
36	29.453742759124907	24.37973117372476	25.687306675665035	20.479219391485294
37	22.39216017096901	29.802148360609635	27.27765592486362	20.528035543557728
38	22.809403295652665	30.37973117372476	24.315392835048648	22.495472695573927
39	22.611832855294978	27.918227321298016	25.6070524717395	23.86288735166751
40	24.044429447162702	24.921545469883583	26.579045048085035	24.454980034868683
41	19.616781958270064	24.313705640852596	27.940273325459756	28.12923907541758
42	22.476238681738934	25.602665766829762	24.96400652381756	26.957089027613744
43	23.4112254653844	25.3344018896575	25.80034868680052	25.454023958157585
44	22.250885776952927	26.945109948821777	27.566166132388503	23.237838141836793
45	20.502671390810416	31.587649738484902	24.18165457510826	23.728024295596423
46	24.040661380124853	28.891682132613468	26.823182048253752	20.24447443900793
47	23.439739047297678	26.285023339519714	27.694899049547267	22.58033856363534
48	23.239637815645914	23.875203869298687	29.05927675608796	23.82588155896744
49	21.18210449356054	23.933805747708227	31.024126877003543	23.859962881727686
50	20.909003993026264	28.524323716326418	27.786738653619032	22.77993363702829
51	20.40852595467072	29.279455598672737	25.64692649457286	24.665091952083685
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	922.0
1	1457.5
2	1993.0
3	9089.0
4	16185.0
5	11980.5
6	7776.0
7	7910.5
8	8045.0
9	8358.5
10	8672.0
11	8574.0
12	8476.0
13	8249.5
14	8023.0
15	7542.5
16	7062.0
17	6600.0
18	6138.0
19	6020.5
20	5903.0
21	5753.5
22	5604.0
23	6724.5
24	7845.0
25	7890.0
26	8416.0
27	8897.0
28	13104.0
29	17311.0
30	18746.5
31	20182.0
32	26558.5
33	32935.0
34	35620.5
35	38306.0
36	40284.5
37	42263.0
38	50201.0
39	58139.0
40	76164.5
41	94190.0
42	113296.0
43	132402.0
44	140450.5
45	148499.0
46	149970.5
47	151442.0
48	148842.0
49	146242.0
50	139822.5
51	133403.0
52	124906.0
53	116409.0
54	108044.0
55	99679.0
56	94753.5
57	89828.0
58	89200.0
59	88572.0
60	81750.0
61	74928.0
62	66536.5
63	58145.0
64	51526.0
65	44907.0
66	37532.0
67	30157.0
68	24822.0
69	19487.0
70	15903.0
71	12319.0
72	9979.0
73	7639.0
74	6426.0
75	4168.0
76	3123.0
77	2249.0
78	1375.0
79	1122.5
80	870.0
81	614.0
82	358.0
83	269.5
84	181.0
85	117.5
86	54.0
87	47.0
88	40.0
89	26.0
90	12.0
91	8.5
92	5.0
93	5.5
94	6.0
95	4.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1778100.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.937530444289568
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.02304576941441	15.343763959473522
2	7.0047925207775545	2.6530694323631354
3	2.325951082040519	1.3214330828421177
4	1.2284325033213555	0.9305391172161205
5	0.7323203681557753	0.693416963346167
6	0.5640258730165271	0.6408754284970489
7	0.435112852985197	0.5767974030076201
8	0.34690714521282195	0.5255651699047518
9	0.2916252697861254	0.4970396182409017
>10	3.401216472997823	14.900145973741402
>50	1.210798733404271	16.672732637965577
>100	1.4238364844579878	42.619139682042885
>500	0.00805607399000612	1.0661833856711227
>1k	0.003580477328891608	1.0558430624119244
>5k	2.9837311074096734E-4	0.5034550832757276
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8910	0.5010966762274338	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3750	0.21089927450649568	No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	1996	0.11225465384399078	No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	1809	0.10173781002193352	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.057308362859231764	0.0
2	0.0	0.0	0.0	0.23643214667341544	0.0
3	0.0	0.0	0.0	0.3472808053540296	0.0
4	0.0	0.0	0.0	0.5007592373882234	0.0
5	0.0	0.0	0.0	0.8189078229570891	0.0
6	0.0	0.0	0.0	1.121927900568022	0.0
7	0.0	0.0	0.0	1.309881334008211	0.0
8	0.0	0.0	0.0	1.7578313930600078	0.0
9	0.0	0.0	0.0	1.977729036612114	0.0
10	0.0	0.0	0.0	2.361059557955121	0.0
11	0.0	0.0	0.0	2.6751588774534616	0.0
12	0.0	0.0	0.0	2.9800911084865866	0.0
13	0.0	0.0	0.0	3.098757100275575	0.0
14	0.0	0.0	0.0	3.1439176649232325	0.0
15	0.0	0.0	0.0	3.220628761037062	0.0
16	0.0	0.0	0.0	3.3877734660592767	0.0
17	0.0	0.0	0.0	3.583712952027445	0.0
18	0.0	0.0	0.0	3.9091727124458693	0.0
19	0.0	0.0	0.0	4.031550531466172	0.0
20	0.0	0.0	0.0	4.167988302120241	0.0
21	0.0	0.0	0.0	4.321185535121759	0.0
22	0.0	0.0	0.0	4.470502221472358	0.0
23	0.0	0.0	0.0	4.635959732298521	0.0
24	0.0	0.0	0.0	4.761599460097857	0.0
25	0.0	0.0	0.0	4.860806478825713	0.0
26	0.0	0.0	0.0	4.9573139868398854	0.0
27	0.0	0.0	0.0	5.062707384286598	0.0
28	0.0	0.0	0.0	5.168606939992126	0.0
29	0.0	0.0	0.0	5.278836960800855	0.0
30	0.0	0.0	0.0	5.426691412181542	0.0
31	0.0	0.0	0.0	5.55801136044092	0.0
32	0.0	0.0	0.0	5.680614138687363	0.0
33	0.0	0.0	0.0	5.800911084865868	0.0
34	0.0	0.0	0.0	5.928687925313537	0.0
35	0.0	0.0	0.0	6.081210280636634	0.0
36	0.0	0.0	0.0	6.2112367133457065	0.0
37	0.0	0.0	0.0	6.341881783926663	0.0
38	0.0	0.0	0.0	6.473595410831787	0.0
39	0.0	0.0	0.0	6.6058714358022605	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAATC	70	0.0	45.000004	13
TAATACG	20	7.03433E-4	45.0	1
ACGTTAG	45	3.8562575E-10	45.0	1
ATAGACG	40	6.8193913E-9	45.0	1
TATGCGT	75	0.0	45.0	20
CGGATTA	25	3.8916703E-5	45.0	20
ACGTGTA	45	3.8562575E-10	45.0	21
ACTACCG	25	3.8916703E-5	45.0	23
CGTACGT	20	7.03433E-4	45.0	21
TATCGCC	20	7.03433E-4	45.0	6
TAGTCGA	20	7.03433E-4	45.0	12
ATTTCGC	25	3.8916703E-5	45.0	42
TATATCG	30	2.1661745E-6	44.999996	21
TAGCACG	30	2.1661745E-6	44.999996	1
CATCGAA	30	2.1661745E-6	44.999996	29
TAATGCG	30	2.1661745E-6	44.999996	1
CGTTTTT	7320	0.0	43.186478	1
CGTTGAT	215	0.0	42.906975	25
CTAACGG	105	0.0	42.857143	2
GCGTAAG	100	0.0	42.75	1
>>END_MODULE