FastQCFastQC Report
Thu 26 May 2016
SRR1546365_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546365_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2469924
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158130.6402221282922066No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC52040.21069474202445093No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG27510.11137994529386329No Hit
GTACAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG25850.10465909072505875No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTT253.8921127E-545.021
CGTTTTT112200.043.1751371
CGCATCG1000.042.7521
CGTAAGG1500.042.0000042
TATAGCG750.042.0000041
CGGTCTA5450.041.69724731
CACGACG5600.041.38392626
TCACGAC5750.040.30434825
ACGGTCT5700.040.26315730
ACTAACG451.9297659E-840.01
TACAAGG8900.039.691012
TAGTAGG10050.039.6268652
TTACGGG6150.039.5121963
CGCGATA403.4603727E-739.37511
AGGGCGA26550.039.0677956
GTACAAG7800.038.942311
GTGCAAG5700.038.684211
AACGGGA6350.038.6220474
CAATTCG356.2512045E-638.57142632
TAACGCC1750.038.57142612
Produced by FastQC (version 0.11.5)