##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546365_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2469924
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.199550269562952	31.0	31.0	33.0	30.0	34.0
2	31.540818664865803	31.0	31.0	34.0	30.0	34.0
3	31.597704220858617	31.0	31.0	34.0	30.0	34.0
4	35.459298342783015	37.0	35.0	37.0	33.0	37.0
5	35.37687839787783	37.0	35.0	37.0	33.0	37.0
6	35.41838493816004	37.0	35.0	37.0	33.0	37.0
7	35.82338241986393	37.0	35.0	37.0	35.0	37.0
8	35.72729606255091	37.0	35.0	37.0	35.0	37.0
9	37.472647336517234	39.0	37.0	39.0	35.0	39.0
10	36.988377780045056	39.0	37.0	39.0	33.0	39.0
11	36.72370121509812	39.0	35.0	39.0	32.0	39.0
12	36.13656493074281	38.0	35.0	39.0	32.0	39.0
13	35.89964144645746	38.0	35.0	39.0	31.0	39.0
14	36.800574025759495	39.0	35.0	41.0	31.0	41.0
15	37.04247175216727	39.0	35.0	41.0	32.0	41.0
16	37.11494199821533	39.0	35.0	41.0	32.0	41.0
17	37.03557396907759	38.0	35.0	41.0	32.0	41.0
18	36.92485072415184	38.0	35.0	40.0	32.0	41.0
19	36.8318802521859	38.0	35.0	40.0	31.0	41.0
20	36.64021848445539	38.0	35.0	40.0	31.0	41.0
21	36.51044445092238	38.0	35.0	40.0	31.0	41.0
22	36.41742053601649	38.0	35.0	40.0	31.0	41.0
23	36.361449987934854	38.0	35.0	40.0	31.0	41.0
24	36.35079540908951	38.0	35.0	40.0	31.0	41.0
25	36.27537486983405	38.0	35.0	40.0	31.0	41.0
26	36.172396802492706	38.0	35.0	40.0	30.0	41.0
27	36.0798834296116	38.0	35.0	40.0	30.0	41.0
28	36.02414730169835	38.0	35.0	40.0	30.0	41.0
29	35.90209496324583	38.0	35.0	40.0	30.0	41.0
30	35.7267944276828	37.0	34.0	40.0	29.0	41.0
31	35.507707929474755	37.0	34.0	40.0	29.0	41.0
32	35.32213015461205	37.0	34.0	40.0	28.0	41.0
33	35.094346627669516	37.0	34.0	40.0	27.0	41.0
34	34.84705885687171	37.0	34.0	40.0	25.0	41.0
35	34.64780859653981	37.0	33.0	40.0	24.0	41.0
36	34.446694716112724	37.0	33.0	40.0	23.0	41.0
37	34.25166968700252	37.0	33.0	40.0	23.0	41.0
38	34.13652525340861	36.0	33.0	40.0	23.0	41.0
39	34.05577094679836	36.0	33.0	40.0	23.0	41.0
40	33.805318706162616	36.0	33.0	40.0	22.0	41.0
41	33.67727428050418	36.0	33.0	40.0	21.0	41.0
42	33.579352239178206	36.0	33.0	40.0	21.0	41.0
43	33.54797475549855	35.0	32.0	40.0	21.0	41.0
44	33.29654961043336	35.0	32.0	40.0	20.0	41.0
45	33.274446096317135	35.0	32.0	40.0	20.0	41.0
46	33.22005454418841	35.0	32.0	39.0	20.0	41.0
47	33.15173179417666	35.0	32.0	39.0	20.0	41.0
48	33.062331472547335	35.0	32.0	39.0	20.0	41.0
49	33.03360670206857	35.0	32.0	39.0	20.0	41.0
50	32.89287362688083	35.0	32.0	39.0	20.0	41.0
51	32.716831772961434	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	4.0
12	7.0
13	13.0
14	45.0
15	112.0
16	278.0
17	793.0
18	1753.0
19	3287.0
20	5571.0
21	8858.0
22	13173.0
23	18453.0
24	26389.0
25	36891.0
26	47244.0
27	54493.0
28	58250.0
29	63572.0
30	73342.0
31	87340.0
32	107212.0
33	137597.0
34	222954.0
35	287097.0
36	195574.0
37	245821.0
38	333688.0
39	440030.0
40	81.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.238178988503286	23.433352605181373	28.161068923578213	14.16739948273712
2	34.35806121969745	24.8438818360403	28.110419591857887	12.687637352404366
3	29.90832916316453	24.776875725730832	31.827295090861096	13.487500020243537
4	26.728595697681385	27.436309781191646	30.193074766672982	15.642019754453983
5	23.47971030687584	31.360964952767777	30.085581580647826	15.073743159708558
6	20.737763591106447	41.44423067268467	27.66012233574798	10.157883400460904
7	85.63461871701315	4.435237683426696	8.079398394444526	1.850745205115623
8	86.44423877009982	3.7148916322931393	7.696957477234117	2.1439121203729345
9	81.77668624621649	5.2493113148420765	9.654548075163447	3.3194543637779947
10	45.42091173655546	25.068828028716673	15.49569946281748	14.014560771910393
11	38.694469951302146	23.770609945893074	21.84508511193057	15.689834990874212
12	34.0555013028741	22.04723708097901	28.06794864943213	15.829312966714765
13	24.41795779951124	30.300608439773857	28.65938385148693	16.622049909227975
14	18.882321885207805	33.897237323901464	30.114246430254532	17.1061943606362
15	17.62483380055419	23.28553429174339	41.559861760928676	17.529770146773746
16	20.87303091107257	19.40841094705748	38.64442792571755	21.0741302161524
17	21.157371643823858	19.35930012421435	29.275030324819713	30.208297907142086
18	24.277832030459237	22.900056843854305	31.39023710851022	21.431874017176238
19	28.79303168842442	24.66136609871397	25.79597590857047	20.749626304291144
20	29.519896158748203	23.939683974081795	25.003157991905823	21.53726187526418
21	25.955859370571726	25.337662211468853	28.21354017370575	20.49293824425367
22	23.989402103060662	22.722763939295298	27.4361883199645	25.85164563767954
23	22.308459693496644	27.191524921414583	26.629807232935104	23.87020815215367
24	22.76770459333971	24.974857525980557	32.26358381877337	19.993854061906358
25	22.945118959125868	23.510925842252636	30.4377381652229	23.1062170333986
26	19.92263729572246	28.217669855428753	27.393393480933014	24.466299367915774
27	20.85477123992479	27.68101366681728	29.41726142180893	22.046953671449
28	19.516430465066943	26.201332510635954	33.61508289323882	20.667154131058282
29	20.70788412922827	26.037643263517417	32.09787021786905	21.15660238938526
30	22.67644672467655	25.93921918245258	30.600091338842816	20.78424275402806
31	28.579219441569865	25.06951630900384	25.889217643943702	20.4620466054826
32	28.449741773431086	26.08914282382778	25.709576488993186	19.751538913747954
33	27.06249261110868	25.781157638858527	25.51827505623655	21.63807469379625
34	22.006466595733308	27.101279229644316	28.266254346287578	22.625999828334802
35	22.323237476132867	26.035821345110215	28.645942142349316	22.9949990364076
36	29.13239435707333	25.78184591914569	25.283207094631248	19.802552629149723
37	22.890258971531107	29.744680403121716	26.137929750065187	21.227130875281993
38	23.779679050853385	29.33742090849759	23.94199173739759	22.940908303251437
39	22.99904774397917	27.017835366594277	25.735690652829803	24.247426236596752
40	25.142028661610638	24.422249429537104	26.69094271726579	23.744779191586463
41	21.09291621928448	24.055760420158677	26.867547341537634	27.983776019019206
42	23.184600011984173	26.137079521475155	25.19814374855259	25.480176717988083
43	23.154356166424552	25.891889790940937	26.076591830355916	24.8771622122786
44	22.981516840194274	26.9822877141159	27.562791405727467	22.473404039962364
45	21.68135537773632	29.887721241625247	24.950686741778288	23.480236638860145
46	24.037986593919488	27.186099653268688	26.9146742976707	21.861239455141128
47	23.239540973730367	26.720336334235384	27.33594232049245	22.704180371541796
48	23.15042892008013	25.89472388624103	28.147262830759168	22.80758436291967
49	22.92884315468816	24.302205250040082	29.610020389291332	23.158931205980426
50	21.577303593146997	27.515300065912957	27.63805687948293	23.269339461457115
51	21.497584541062803	28.7532328929959	25.652935070066935	24.09624749587437
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1379.0
1	2354.5
2	3330.0
3	15840.5
4	28351.0
5	20388.0
6	12425.0
7	12644.5
8	12864.0
9	13201.5
10	13539.0
11	13420.0
12	13301.0
13	12779.0
14	12257.0
15	11685.5
16	11114.0
17	10734.5
18	10355.0
19	10150.5
20	9946.0
21	10354.0
22	10762.0
23	10594.5
24	10427.0
25	11679.5
26	14920.0
27	16908.0
28	19516.0
29	22124.0
30	26367.0
31	30610.0
32	36292.5
33	41975.0
34	47599.5
35	53224.0
36	56428.0
37	59632.0
38	67742.5
39	75853.0
40	95694.0
41	115535.0
42	136221.5
43	156908.0
44	166871.0
45	176834.0
46	185058.0
47	193282.0
48	193173.0
49	193064.0
50	187577.0
51	182090.0
52	171822.0
53	161554.0
54	152968.0
55	144382.0
56	143153.0
57	141924.0
58	136665.0
59	131406.0
60	122372.0
61	113338.0
62	100963.5
63	88589.0
64	80514.0
65	72439.0
66	60053.5
67	47668.0
68	40058.0
69	32448.0
70	27447.5
71	22447.0
72	18866.5
73	15286.0
74	11971.5
75	6666.0
76	4675.0
77	3422.0
78	2169.0
79	1539.5
80	910.0
81	728.0
82	546.0
83	392.0
84	238.0
85	171.0
86	104.0
87	86.5
88	69.0
89	49.5
90	30.0
91	21.0
92	12.0
93	8.5
94	5.0
95	6.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2469924.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.770071320440156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.76414968898534	15.96712999485945
2	6.753250381494401	2.6702448337386797
3	2.3034559019853016	1.3661846239721467
4	1.2423384492461693	0.9824447898288716
5	0.8282038425322165	0.8186824517362252
6	0.6219821264748787	0.737797860026843
7	0.48540073843843107	0.671748505254547
8	0.3925403214627672	0.620844012117393
9	0.32546656640706706	0.5791047507257839
>10	3.8140985856362852	17.275466645358087
>50	1.2375694867464817	17.709187015702526
>100	1.2179082975723972	37.24011063465269
>500	0.009503609073361194	1.3226429954281205
>1k	0.003718803550445685	1.1799713882688831
>5k	2.0660019724698248E-4	0.21255741301364767
>10k+	2.0660019724698248E-4	0.6458820853160666
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15813	0.6402221282922066	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5204	0.21069474202445093	No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	2751	0.11137994529386329	No Hit
GTACAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	2585	0.10465909072505875	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05700580260769157	0.0
2	0.0	0.0	0.0	0.19449991173817494	0.0
3	0.0	0.0	0.0	0.30324819711051837	0.0
4	0.0	0.0	0.0	0.44325250493537455	0.0
5	0.0	0.0	0.0	0.7069853161473795	0.0
6	0.0	0.0	0.0	0.9785321329725125	0.0
7	0.0	0.0	0.0	1.1399541038509686	0.0
8	0.0	0.0	0.0	1.5547441945582132	0.0
9	0.0	0.0	0.0	1.748353390630643	0.0
10	0.0	0.0	0.0	2.082209816982223	0.0
11	0.0	0.0	0.0	2.378858620751084	0.0
12	0.0	0.0	0.0	2.6611749997165903	0.0
13	0.0	0.0	0.0	2.785713244618053	0.0
14	0.0	0.0	0.0	2.8340953000983027	0.0
15	0.0	0.0	0.0	2.9118709725481433	0.0
16	0.0	0.0	0.0	3.075438758439531	0.0
17	0.0	0.0	0.0	3.278521930229432	0.0
18	0.0	0.0	0.0	3.636306218328985	0.0
19	0.0	0.0	0.0	3.751491948740123	0.0
20	0.0	0.0	0.0	3.8929133042150283	0.0
21	0.0	0.0	0.0	4.02384850707957	0.0
22	0.0	0.0	0.0	4.163285995844406	0.0
23	0.0	0.0	0.0	4.3038166356535665	0.0
24	0.0	0.0	0.0	4.414184404054538	0.0
25	0.0	0.0	0.0	4.502162819584732	0.0
26	0.0	0.0	0.0	4.5962952706237115	0.0
27	0.0	0.0	0.0	4.711642949337713	0.0
28	0.0	0.0	0.0	4.824844813038782	0.0
29	0.0	0.0	0.0	4.950111825303127	0.0
30	0.0	0.0	0.0	5.086593757540718	0.0
31	0.0	0.0	0.0	5.2116988215022	0.0
32	0.0	0.0	0.0	5.328706470320545	0.0
33	0.0	0.0	0.0	5.452799357389134	0.0
34	0.0	0.0	0.0	5.586001836493755	0.0
35	0.0	0.0	0.0	5.733941611158886	0.0
36	0.0	0.0	0.0	5.865281684780585	0.0
37	0.0	0.0	0.0	5.997593448219459	0.0
38	0.0	0.0	0.0	6.146423938550336	0.0
39	0.0	0.0	0.0	6.360236185404895	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGTT	25	3.8921127E-5	45.0	21
CGTTTTT	11220	0.0	43.175137	1
CGCATCG	100	0.0	42.75	21
CGTAAGG	150	0.0	42.000004	2
TATAGCG	75	0.0	42.000004	1
CGGTCTA	545	0.0	41.697247	31
CACGACG	560	0.0	41.383926	26
TCACGAC	575	0.0	40.304348	25
ACGGTCT	570	0.0	40.263157	30
ACTAACG	45	1.9297659E-8	40.0	1
TACAAGG	890	0.0	39.69101	2
TAGTAGG	1005	0.0	39.626865	2
TTACGGG	615	0.0	39.512196	3
CGCGATA	40	3.4603727E-7	39.375	11
AGGGCGA	2655	0.0	39.067795	6
GTACAAG	780	0.0	38.94231	1
GTGCAAG	570	0.0	38.68421	1
AACGGGA	635	0.0	38.622047	4
CAATTCG	35	6.2512045E-6	38.571426	32
TAACGCC	175	0.0	38.571426	12
>>END_MODULE