FastQCFastQC Report
Thu 26 May 2016
SRR1546364_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546364_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2467559
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113870.461468195897241No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC74010.29993203809919033No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG30040.1217397436089674No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG27330.11075723012094138No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAGT351.2126839E-745.039
CGCGAAA207.034862E-445.026
ATCGTAC502.1827873E-1145.020
GTTACGA207.034862E-445.035
TACGCCC253.892111E-545.017
GCGATAC950.045.09
CCGGTAT207.034862E-445.042
CCGCGAA207.034862E-445.034
GTGCGTA207.034862E-445.023
AATCGTC207.034862E-445.039
TAATCCG406.8212103E-945.024
GATACGT551.8189894E-1244.9999969
TATAGCG3150.044.285711
CGTTTTT87950.043.618531
GCTACGA1300.043.26923410
ACGTTAG1150.043.0434761
AAGTACG900.042.51
ATATACG850.042.352941
TAGAACG650.041.5384641
AAGTTCG650.041.5384641
Produced by FastQC (version 0.11.5)