##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546364_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2467559
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.142972467932886	31.0	31.0	33.0	30.0	34.0
2	31.472184859612273	31.0	31.0	34.0	30.0	34.0
3	31.56179568553376	31.0	31.0	34.0	30.0	34.0
4	35.41491490173082	37.0	35.0	37.0	33.0	37.0
5	35.32291669621679	37.0	35.0	37.0	33.0	37.0
6	35.360998055163016	37.0	35.0	37.0	33.0	37.0
7	35.79123011850983	37.0	35.0	37.0	35.0	37.0
8	35.69508165762196	37.0	35.0	37.0	35.0	37.0
9	37.42866290127207	39.0	37.0	39.0	35.0	39.0
10	36.93141400063788	39.0	37.0	39.0	32.0	39.0
11	36.62621846124044	39.0	35.0	39.0	32.0	39.0
12	35.898450249821785	37.0	35.0	39.0	31.0	39.0
13	35.5782767504242	37.0	35.0	39.0	30.0	39.0
14	36.48780434429329	38.0	35.0	41.0	31.0	41.0
15	36.76463176766999	38.0	35.0	41.0	31.0	41.0
16	36.87360545381083	38.0	35.0	41.0	32.0	41.0
17	36.818165239412714	38.0	35.0	41.0	32.0	41.0
18	36.737778509044766	38.0	35.0	40.0	31.0	41.0
19	36.63734403108497	38.0	35.0	40.0	31.0	41.0
20	36.48512436784693	38.0	35.0	40.0	31.0	41.0
21	36.323227934975414	38.0	35.0	40.0	31.0	41.0
22	36.20131109327072	38.0	35.0	40.0	30.0	41.0
23	36.15905192135224	38.0	35.0	40.0	30.0	41.0
24	36.15452842262333	38.0	35.0	40.0	30.0	41.0
25	36.100569834399096	37.0	35.0	40.0	30.0	41.0
26	35.985171175238364	37.0	35.0	40.0	30.0	41.0
27	35.87672148872631	37.0	34.0	40.0	30.0	41.0
28	35.80974031421336	37.0	34.0	40.0	30.0	41.0
29	35.734956286759505	37.0	34.0	40.0	30.0	41.0
30	35.584472752221934	37.0	34.0	40.0	29.0	41.0
31	35.36433738767746	37.0	34.0	40.0	29.0	41.0
32	35.176608543098666	37.0	34.0	40.0	27.0	41.0
33	34.97382109201847	37.0	34.0	40.0	27.0	41.0
34	34.76237852874035	36.0	34.0	40.0	25.0	41.0
35	34.54505647078753	36.0	33.0	40.0	24.0	41.0
36	34.370271997549	36.0	33.0	40.0	24.0	41.0
37	34.188912605534455	36.0	33.0	40.0	23.0	41.0
38	34.11319810387513	36.0	33.0	40.0	23.0	41.0
39	34.0985196301284	36.0	33.0	40.0	23.0	41.0
40	33.89187492578698	36.0	33.0	40.0	23.0	41.0
41	33.79939040971259	35.0	33.0	40.0	23.0	41.0
42	33.68925646762651	35.0	33.0	40.0	23.0	41.0
43	33.6299893943772	35.0	33.0	40.0	23.0	41.0
44	33.43101704964299	35.0	32.0	39.0	22.0	41.0
45	33.361604727587064	35.0	32.0	39.0	22.0	41.0
46	33.28397294654353	35.0	32.0	39.0	22.0	41.0
47	33.225596226878466	35.0	32.0	39.0	22.0	41.0
48	33.14713650210593	35.0	32.0	39.0	22.0	41.0
49	33.1386212041941	35.0	32.0	39.0	22.0	41.0
50	32.982335984671494	35.0	32.0	39.0	21.0	40.0
51	32.74323369775556	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	8.0
12	9.0
13	15.0
14	20.0
15	116.0
16	243.0
17	667.0
18	1492.0
19	2902.0
20	5230.0
21	8308.0
22	12448.0
23	18354.0
24	25399.0
25	35079.0
26	45787.0
27	53675.0
28	59176.0
29	65967.0
30	76799.0
31	91492.0
32	112381.0
33	144562.0
34	240084.0
35	316563.0
36	185831.0
37	231904.0
38	317057.0
39	415894.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.138491116119205	23.011445724296763	28.676517967756798	13.173545191827227
2	32.25993785761556	24.652784391376255	30.323043947480084	12.764233803528102
3	29.22483312455751	25.128152964123657	32.026103529844676	13.620910381474161
4	26.882032000045392	26.791740339339405	30.48980794380195	15.836419716813255
5	23.619698657661274	32.085676573488215	29.261468520104284	15.033156248746229
6	20.553470048740476	42.33507689177847	26.612940156648733	10.498512902832314
7	86.31830079848142	4.657558340043744	7.190709523054971	1.8334313384198715
8	87.34984654875527	3.520604775812858	6.848995302645246	2.2805533727866285
9	82.57869416698851	5.091509463400875	8.807327403316394	3.5224689662942206
10	49.65417240276727	22.850963239379485	14.340001596719674	13.154862761133574
11	42.195829967996715	22.005107071401333	20.60254688945634	15.196516071145613
12	38.165166466131105	20.822805047417305	25.94924781940371	15.062780667047882
13	24.377127355414803	32.44092643782783	26.351102445777386	16.83084376097998
14	18.231823433603818	37.22524973060422	27.956656760790725	16.58627007500125
15	16.918703868884187	23.136751745348338	43.252055979208606	16.69248840655887
16	19.153827730157616	17.928973532142496	41.94570423645392	20.971494501245967
17	18.675176561127817	18.52725709901972	29.366714230541195	33.43085210931127
18	22.98429338467692	22.290814525610127	32.51054989971871	22.21434218999424
19	29.23832824260737	24.48541250685394	24.628671492758635	21.647587757780055
20	31.094129866803588	22.53546926334892	24.402293926913195	21.96810694293429
21	24.878675646661335	26.48402733227453	26.235157903012652	22.402139118051483
22	23.392551100095275	24.062241267584685	24.82125047465937	27.723957157660667
23	21.975401601339623	28.519358605001948	24.778130938307857	24.727108855350572
24	22.004985493761243	23.554168309653388	34.107472202285734	20.33337399429963
25	21.31924707778011	22.92869187727629	31.807425881204864	23.944635163738738
26	19.50425501477371	29.934319706236	26.89418976405427	23.667235514936017
27	20.628564504435353	28.242080533839314	28.832096821190493	22.297258140534836
28	18.580183898338397	26.965798994066603	34.847636875146655	19.60638023244834
29	19.601962911525113	24.923537795854124	33.656013898755816	21.81848539386495
30	22.407366956575302	26.411931791701836	30.349426295379363	20.831274956343496
31	29.129556780607878	24.71681528182305	24.51896793551846	21.63466000205061
32	29.967064617299933	25.76927238619218	24.812699513973122	19.450963482534764
33	27.630139745392107	25.636306973815014	25.263752558702752	21.46980072209013
34	21.571601732724528	26.392398317527565	27.703977898806066	24.33202205094184
35	22.177828372087557	24.636209306444144	29.78011873272331	23.40584358874499
36	29.02718840765307	25.389099105634354	25.30776366441491	20.275948822297664
37	22.948995343171124	29.58263611933899	26.741528773982708	20.726839763507172
38	22.898378519014134	30.490942668442784	23.57078392046553	23.039894892077555
39	22.24591185053731	27.29944856435044	25.069309386320654	25.3853301987916
40	23.780100090818497	24.532219898288147	26.551421870763782	25.136258140129573
41	19.608892837010178	24.351109740435792	26.74647293134632	29.293524491207705
42	23.375611282242897	25.355097892289507	24.058026576061607	27.211264249405993
43	23.340759025417427	25.37446926294366	25.49503375603177	25.78973795560714
44	22.52505411218131	27.339650237339818	27.241293926507936	22.894001723970938
45	20.586336537444495	30.962785489627603	24.51074118187245	23.94013679105545
46	23.61159348165535	29.184955658608363	26.177327472210393	21.026123387525892
47	23.765875506928104	26.217772300479947	26.392600946927715	23.623751245664238
48	23.10587102476577	24.461340134116348	28.46201448476004	23.97077435635784
49	21.741567273568737	23.669342860697558	30.117739839250046	24.471350026483663
50	20.78730437651136	27.78697490110672	28.692120431568203	22.733600290813715
51	20.68923174683969	29.496153891355792	25.71902029495546	24.09559406684906
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	849.0
1	1861.5
2	2874.0
3	12559.5
4	22245.0
5	16407.5
6	10570.0
7	10492.0
8	10414.0
9	10692.0
10	10970.0
11	10749.0
12	10528.0
13	10110.5
14	9693.0
15	9184.0
16	8675.0
17	8130.5
18	7586.0
19	7482.5
20	7379.0
21	8143.0
22	8907.0
23	9134.5
24	9362.0
25	9313.5
26	12024.0
27	14783.0
28	17993.0
29	21203.0
30	23604.0
31	26005.0
32	32615.0
33	39225.0
34	41630.5
35	44036.0
36	48698.0
37	53360.0
38	65516.0
39	77672.0
40	102926.0
41	128180.0
42	161148.5
43	194117.0
44	197467.0
45	200817.0
46	199868.5
47	198920.0
48	202281.5
49	205643.0
50	195144.5
51	184646.0
52	173182.5
53	161719.0
54	149088.5
55	136458.0
56	135643.5
57	134829.0
58	132674.0
59	130519.0
60	119334.0
61	108149.0
62	98073.5
63	87998.0
64	77217.0
65	66436.0
66	55514.5
67	44593.0
68	37187.0
69	29781.0
70	25187.5
71	20594.0
72	16770.0
73	12946.0
74	10552.5
75	5878.5
76	3598.0
77	2846.0
78	2094.0
79	1482.5
80	871.0
81	639.0
82	407.0
83	328.5
84	250.0
85	177.0
86	104.0
87	73.0
88	42.0
89	44.0
90	46.0
91	36.5
92	27.0
93	19.0
94	11.0
95	7.0
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2467559.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.368662318227233
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.89238031322749	14.858848180912885
2	7.522713733868451	2.7636437658823976
3	2.499255341043462	1.377239322199596
4	1.2543904305362676	0.9216589693494549
5	0.7818255154828752	0.7180544442839435
6	0.562768392859801	0.6202381543087875
7	0.40265659282963123	0.5177384088726789
8	0.3335830978484396	0.4901980223556912
9	0.2921172353422336	0.48292225680020423
>10	3.0411102422435246	12.891514835186365
>50	0.9659344124688133	12.869701033529488
>100	1.4335013546415716	47.85721982239332
>500	0.012878419765410414	1.6022816937355324
>1k	0.004440834401865659	1.2624519604479048
>5k	2.22041720093283E-4	0.30185787998820607
>10k+	2.22041720093283E-4	0.46443124975350664
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11387	0.461468195897241	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7401	0.29993203809919033	No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	3004	0.1217397436089674	No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	2733	0.11075723012094138	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07217659233274665	0.0
2	0.0	0.0	0.0	0.2551509406664643	0.0
3	0.0	0.0	0.0	0.36217978982468096	0.0
4	0.0	0.0	0.0	0.5292679931867891	0.0
5	0.0	0.0	0.0	0.8627554599505017	0.0
6	0.0	0.0	0.0	1.2025649639988345	0.0
7	0.0	0.0	0.0	1.4227825960797695	0.0
8	0.0	0.0	0.0	2.04696220029592	0.0
9	0.0	0.0	0.0	2.3252534184592952	0.0
10	0.0	0.0	0.0	2.7834390180741373	0.0
11	0.0	0.0	0.0	3.1249911349637434	0.0
12	0.0	0.0	0.0	3.475377893699806	0.0
13	0.0	0.0	0.0	3.605222813314697	0.0
14	0.0	0.0	0.0	3.653570188190029	0.0
15	0.0	0.0	0.0	3.731015144926626	0.0
16	0.0	0.0	0.0	3.9140705450204027	0.0
17	0.0	0.0	0.0	4.127398777496303	0.0
18	0.0	0.0	0.0	4.47944709731358	0.0
19	0.0	0.0	0.0	4.602402617323436	0.0
20	0.0	0.0	0.0	4.7475257937094915	0.0
21	0.0	0.0	0.0	4.904806734104433	0.0
22	0.0	0.0	0.0	5.065775529582068	0.0
23	0.0	0.0	0.0	5.251262482477623	0.0
24	0.0	0.0	0.0	5.380945298572395	0.0
25	0.0	0.0	0.0	5.489270975891559	0.0
26	0.0	0.0	0.0	5.596745609730101	0.0
27	0.0	0.0	0.0	5.701829216646897	0.0
28	0.0	0.0	0.0	5.817084819451126	0.0
29	0.0	0.0	0.0	5.937365631379027	0.0
30	0.0	0.0	0.0	6.092782381292605	0.0
31	0.0	0.0	0.0	6.239607644639905	0.0
32	0.0	0.0	0.0	6.374396721618409	0.0
33	4.0525880029616314E-5	0.0	0.0	6.517088345202688	0.0
34	4.0525880029616314E-5	0.0	0.0	6.652768991541843	0.0
35	4.0525880029616314E-5	0.0	0.0	6.828934992030586	0.0
36	4.0525880029616314E-5	0.0	0.0	6.978516015219899	0.0
37	4.0525880029616314E-5	0.0	0.0	7.118735560122372	0.0
38	4.0525880029616314E-5	0.0	0.0	7.265358194069524	0.0
39	4.0525880029616314E-5	0.0	0.0	7.4134397596977415	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACAGT	35	1.2126839E-7	45.0	39
CGCGAAA	20	7.034862E-4	45.0	26
ATCGTAC	50	2.1827873E-11	45.0	20
GTTACGA	20	7.034862E-4	45.0	35
TACGCCC	25	3.892111E-5	45.0	17
GCGATAC	95	0.0	45.0	9
CCGGTAT	20	7.034862E-4	45.0	42
CCGCGAA	20	7.034862E-4	45.0	34
GTGCGTA	20	7.034862E-4	45.0	23
AATCGTC	20	7.034862E-4	45.0	39
TAATCCG	40	6.8212103E-9	45.0	24
GATACGT	55	1.8189894E-12	44.999996	9
TATAGCG	315	0.0	44.28571	1
CGTTTTT	8795	0.0	43.61853	1
GCTACGA	130	0.0	43.269234	10
ACGTTAG	115	0.0	43.043476	1
AAGTACG	90	0.0	42.5	1
ATATACG	85	0.0	42.35294	1
TAGAACG	65	0.0	41.538464	1
AAGTTCG	65	0.0	41.538464	1
>>END_MODULE