FastQCFastQC Report
Thu 26 May 2016
SRR1546363_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546363_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2628011
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100850.38375029632676577No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC55040.20943595745984317No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG54900.2089032351843276No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG40320.1534240153484898No Hit
GTGCAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG33390.1270542627104681No Hit
GTACAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG32880.12511363156394703No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28360.10791431238301515No Hit
GGGCAAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28320.10776210601858212No Hit
GTACGAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG28100.10692497101420047No Hit
GGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG27270.10376668895221519No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG253.89218E-545.0000041
GTCGAAA253.89218E-545.00000425
GTACGAC207.0349453E-445.034
CGTTTTT80650.043.2424051
TACGTAG1150.043.0434761
TGTAACG950.042.631581
AATACGG2450.041.326532
TACGCGG2050.040.6097532
CGACGGT6000.040.528
CGCATCG3000.039.7521
TACGGGA6150.039.5121964
GTACGAG4800.039.3750041
CATATGC19150.039.36031333
CACGACG6200.039.1935526
CGGTCTA6250.038.8831
GCGATAT2900.038.7931029
TATAGCG1450.038.7931021
CTCGCTA356.2513573E-638.57142638
ACGGTCT6500.038.42307730
TAGCATA19900.038.10301630