FastQCFastQC Report
Thu 26 May 2016
SRR1546362_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546362_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1860760
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85890.4615855886841935No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC51620.27741352995550206No Hit
GTACAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG21120.11350200993142587No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG18680.10038908832949978No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAC453.8562575E-1045.0000049
TATACGA453.8562575E-1045.00000422
ACGCATA207.034414E-445.032
GTTACGT253.8917402E-545.045
GTTACGA253.8917402E-545.034
TCGCTAA253.8917402E-545.015
CGTACGT253.8917402E-545.027
ACGTCGT207.034414E-445.031
CGTAATC253.8917402E-545.034
TTGACGT302.166229E-644.99999612
CGTTTTT71850.043.340291
CGTTAGG3950.041.5822752
CTATGCG603.6379788E-1241.2499961
CGGTCTA2350.041.1702131
ACGTAAG556.184564E-1140.9090921
CTAACGG1050.040.7142872
CTATACG501.0822987E-940.51
GGCGTAA501.0822987E-940.58
TATACGG3700.040.1351362
TGCAACG403.4597542E-739.3751