##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546362_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1860760
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.084187106343645	31.0	31.0	33.0	30.0	34.0
2	31.411042262301425	31.0	31.0	34.0	30.0	34.0
3	31.50148971388035	31.0	31.0	34.0	30.0	34.0
4	35.35160579548142	37.0	35.0	37.0	33.0	37.0
5	35.266501859455275	37.0	35.0	37.0	33.0	37.0
6	35.31023452782734	37.0	35.0	37.0	32.0	37.0
7	35.738869601668135	37.0	35.0	37.0	35.0	37.0
8	35.63985253337346	37.0	35.0	37.0	35.0	37.0
9	37.40081310862228	39.0	37.0	39.0	35.0	39.0
10	36.84867151056557	39.0	37.0	39.0	32.0	39.0
11	36.53709290827404	39.0	35.0	39.0	32.0	39.0
12	35.75776779380468	37.0	35.0	39.0	31.0	39.0
13	35.37103065414132	37.0	35.0	39.0	30.0	39.0
14	36.266457791440054	38.0	35.0	40.0	30.0	41.0
15	36.578345407252954	38.0	35.0	40.0	31.0	41.0
16	36.69336830112427	38.0	35.0	40.0	31.0	41.0
17	36.631748855306434	38.0	35.0	40.0	31.0	41.0
18	36.562074636170166	38.0	35.0	40.0	31.0	41.0
19	36.42530417678798	38.0	35.0	40.0	31.0	41.0
20	36.25007093875621	37.0	35.0	40.0	31.0	41.0
21	36.06954900148327	37.0	34.0	40.0	30.0	41.0
22	35.96049624884456	37.0	34.0	40.0	30.0	41.0
23	35.91630355338679	37.0	34.0	40.0	30.0	41.0
24	35.87287022506933	37.0	34.0	40.0	30.0	41.0
25	35.787252520475505	37.0	34.0	40.0	30.0	41.0
26	35.65523979449257	37.0	34.0	40.0	30.0	41.0
27	35.53774855435414	37.0	34.0	40.0	29.0	41.0
28	35.4935623078742	36.0	34.0	40.0	29.0	41.0
29	35.423040048152366	36.0	34.0	40.0	29.0	41.0
30	35.27145951116748	36.0	34.0	40.0	29.0	41.0
31	35.06066660934242	36.0	34.0	40.0	27.0	41.0
32	34.8488735785378	36.0	34.0	40.0	27.0	41.0
33	34.66404856080311	36.0	33.0	40.0	25.0	41.0
34	34.45434553623251	36.0	33.0	40.0	24.0	41.0
35	34.288162901180165	36.0	33.0	40.0	24.0	41.0
36	34.07706421032266	36.0	33.0	40.0	23.0	41.0
37	33.88124368537587	35.0	33.0	40.0	23.0	41.0
38	33.8108864120037	35.0	33.0	40.0	23.0	41.0
39	33.79128420645328	35.0	33.0	40.0	23.0	41.0
40	33.56565274404007	35.0	32.0	40.0	22.0	41.0
41	33.47485543541349	35.0	32.0	40.0	22.0	41.0
42	33.3525957135794	35.0	32.0	39.0	21.0	41.0
43	33.27379887787786	35.0	32.0	39.0	22.0	41.0
44	33.0767170403491	35.0	32.0	39.0	21.0	41.0
45	33.02645316967261	35.0	32.0	39.0	21.0	41.0
46	32.95394408736215	35.0	32.0	39.0	20.0	41.0
47	32.88610513983534	35.0	31.0	39.0	20.0	41.0
48	32.8100104258475	35.0	31.0	39.0	20.0	41.0
49	32.823630667039275	35.0	31.0	39.0	20.0	40.0
50	32.65905866420172	35.0	31.0	39.0	20.0	40.0
51	32.43360777316795	35.0	31.0	38.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	0.0
12	4.0
13	15.0
14	37.0
15	97.0
16	266.0
17	638.0
18	1353.0
19	2724.0
20	4647.0
21	7169.0
22	10937.0
23	15827.0
24	21728.0
25	29498.0
26	37670.0
27	43250.0
28	47278.0
29	52528.0
30	60213.0
31	71531.0
32	87559.0
33	111740.0
34	186975.0
35	248133.0
36	138484.0
37	168629.0
38	226915.0
39	284862.0
40	49.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.078118618199014	23.24168619273845	28.567252090543647	14.112943098518885
2	31.204024162170295	24.96925987231024	30.66811410391453	13.158601861604936
3	29.651432747909457	24.740374900578257	32.37918914851996	13.229003202992326
4	25.834121541735634	27.77580128549625	31.422053354543305	14.968023818224813
5	22.867108063371955	32.93879920032675	30.10355983576603	14.090532900535266
6	20.50404135944453	41.46047851415551	27.192168791246583	10.843311335153379
7	86.83317569165287	4.081181882671596	7.201573550592231	1.8840688750832995
8	88.40468410756895	3.100990992927621	6.35960575248823	2.1347191470151983
9	84.50654571250456	4.169156688664847	8.401728326060319	2.922569272770266
10	51.7111287860874	21.00088136030439	14.26180700358993	13.026182850018273
11	43.69456566134268	21.048173864442486	19.93959457425998	15.317665899954857
12	38.09620800103183	19.754885100711537	25.7271222511232	16.421784647133432
13	23.328747393538123	33.82639351662761	26.1915023968701	16.653356692964167
14	18.036178765665642	36.93727294223865	28.21239708506202	16.814151207033685
15	16.3434833078957	21.67329478277693	45.424020292783595	16.559201616543778
16	18.034136589350588	16.94130355338679	43.25528278767815	21.76927706958447
17	18.80075882972549	17.121230035039446	29.349835551065155	34.728175584169904
18	23.740407145467444	21.305971753477074	32.88629377243707	22.067327328618415
19	29.480266127818737	23.250016122444592	24.95781293664954	22.311904813087125
20	31.488262860336636	22.403211590962833	23.97273157204583	22.1357939766547
21	24.194630151121046	25.968421505191426	26.96699198177089	22.869956361916636
22	23.796943184505256	22.95411552268965	25.244899933360564	28.004041359444525
23	21.962208989875105	27.849857047657945	24.3704722801436	25.81746168232335
24	23.04671209613276	23.377383434725594	33.43811130935747	20.137793159784174
25	21.308927534985706	22.13063479438509	32.12724908102066	24.433188589608548
26	19.543466110621466	28.85992820138008	27.285732711365245	24.310872976633206
27	19.784335432833895	27.554493862722758	29.564908961929536	23.096261742513814
28	18.154839957866677	25.507910746146734	36.05155957780692	20.28568971817967
29	18.94032545841484	23.890722070551817	34.64922934714848	22.519723123884862
30	21.50986693609063	25.65043315634472	30.88985145854382	21.94984844902083
31	29.309636922547778	23.938283282099786	23.809035017949657	22.943044777402783
32	29.454308992024764	25.020690470560414	25.559663793288763	19.965336744126056
33	27.059319847804126	25.74722156538189	24.931855800855566	22.261602785958427
34	20.54477740278166	27.163632064317806	27.913164513424622	24.378426019475913
35	21.522227476944906	25.299017605709494	28.933715256131904	24.245039661213696
36	28.92957716201982	24.538521894279757	25.753563060254947	20.778337883445474
37	22.315290526451555	29.175659407983833	26.70129409488596	21.807755970678645
38	22.36027214686472	30.02971903953223	22.45915647369892	25.150852339904123
39	21.79630903501795	27.559223113136568	25.02042176315054	25.62404608869494
40	23.490670478729122	24.320438960424774	24.699961306132977	27.488929254713128
41	19.836410928867775	22.48199660353834	26.468647219415725	31.212945248178166
42	23.773888088737934	24.659816419097574	23.4050065564608	28.161288935703695
43	23.484812657193835	25.609052215223887	24.407500161224448	26.49863496635783
44	22.086996711021303	27.81159311249167	26.490520002579593	23.610890173907435
45	20.368505341903308	31.58564242567553	23.7834540725295	24.262398159891656
46	23.725359530514414	28.37657731249597	26.36777445774845	21.530288699241172
47	23.542477267353124	26.188761581289366	26.362883982888714	23.9058771684688
48	23.955588039295773	23.393452137836153	27.19985382316903	25.45110599969905
49	21.745953266407277	22.36763472989531	30.21733055310733	25.66908145059008
50	21.13136567853995	27.386712955996472	27.52945033212236	23.952471033341215
51	20.335669296416516	30.14112513166663	24.457479739461295	25.065725832455556
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	630.0
1	1172.5
2	1715.0
3	8804.5
4	15894.0
5	11757.5
6	7621.0
7	7812.0
8	8003.0
9	8330.0
10	8657.0
11	8513.5
12	8370.0
13	7958.5
14	7547.0
15	7242.0
16	6937.0
17	6240.5
18	5544.0
19	5202.0
20	4860.0
21	5183.5
22	5507.0
23	5091.0
24	4675.0
25	5460.0
26	6650.5
27	7056.0
28	9265.0
29	11474.0
30	13323.0
31	15172.0
32	19229.5
33	23287.0
34	25814.0
35	28341.0
36	31140.0
37	33939.0
38	42811.0
39	51683.0
40	71540.0
41	91397.0
42	115357.5
43	139318.0
44	150638.5
45	161959.0
46	160413.0
47	158867.0
48	158385.5
49	157904.0
50	154775.5
51	151647.0
52	140565.5
53	129484.0
54	120450.5
55	111417.0
56	106575.0
57	101733.0
58	98634.0
59	95535.0
60	90857.5
61	86180.0
62	76966.0
63	67752.0
64	57941.0
65	48130.0
66	42054.0
67	35978.0
68	29402.5
69	22827.0
70	19022.5
71	15218.0
72	12730.5
73	10243.0
74	8367.5
75	4633.5
76	2775.0
77	2135.0
78	1495.0
79	1051.5
80	608.0
81	495.5
82	383.0
83	258.0
84	133.0
85	98.0
86	63.0
87	43.5
88	24.0
89	18.5
90	13.0
91	12.5
92	12.0
93	12.5
94	13.0
95	7.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1860760.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.70237046148081
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.33768182489942	18.33086385245897
2	8.497243944245508	4.028096477361628
3	3.597115734588187	2.557805092020927
4	2.0333067285047366	1.927767573633634
5	1.3678238050065057	1.6210333276148248
6	0.9615804218188019	1.3675041231873737
7	0.7328604653787578	1.2159371172886386
8	0.5529721112917435	1.048539986936328
9	0.42941920149546414	0.9160427697406888
>10	2.623168933948086	13.103985426649079
>50	0.8537618358882304	14.810012726079178
>100	1.0048583459013363	36.776678805969595
>500	0.005927022857312724	0.9202274518066041
>1k	0.0018236993407116076	0.6325044067199875
>5k	4.559248351779019E-4	0.7430008625325943
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8589	0.4615855886841935	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5162	0.27741352995550206	No Hit
GTACAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	2112	0.11350200993142587	No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	1868	0.10038908832949978	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.059276854618542964	0.0
2	0.0	0.0	0.0	0.21781422644510845	0.0
3	0.0	0.0	0.0	0.311055697671919	0.0
4	0.0	0.0	0.0	0.46104817386444247	0.0
5	5.3741481975106946E-5	0.0	0.0	0.7779616930716482	0.0
6	5.3741481975106946E-5	0.0	0.0	1.0986908574990863	0.0
7	5.3741481975106946E-5	0.0	0.0	1.3201595047185022	0.0
8	5.3741481975106946E-5	0.0	0.0	1.9881123841871065	0.0
9	5.3741481975106946E-5	0.0	0.0	2.2791762505642854	0.0
10	5.3741481975106946E-5	0.0	0.0	2.776768632171801	0.0
11	5.3741481975106946E-5	0.0	0.0	3.0836862357316366	0.0
12	5.3741481975106946E-5	0.0	0.0	3.4212902254992583	0.0
13	5.3741481975106946E-5	0.0	0.0	3.589930995937144	0.0
14	5.3741481975106946E-5	0.0	0.0	3.631634385949827	0.0
15	5.3741481975106946E-5	0.0	0.0	3.725628237924289	0.0
16	5.3741481975106946E-5	0.0	0.0	3.8916894172273695	0.0
17	5.3741481975106946E-5	0.0	0.0	4.074356714460758	0.0
18	5.3741481975106946E-5	0.0	0.0	4.388421935123283	0.0
19	5.3741481975106946E-5	0.0	0.0	4.501064081343107	0.0
20	5.3741481975106946E-5	0.0	0.0	4.633859283303597	0.0
21	5.3741481975106946E-5	0.0	0.0	4.775038156452203	0.0
22	5.3741481975106946E-5	0.0	0.0	4.927448999333605	0.0
23	5.3741481975106946E-5	0.0	0.0	5.1003890883295	0.0
24	5.3741481975106946E-5	0.0	0.0	5.225499258367549	0.0
25	5.3741481975106946E-5	0.0	0.0	5.332552290461962	0.0
26	5.3741481975106946E-5	0.0	0.0	5.429071992089254	0.0
27	5.3741481975106946E-5	0.0	0.0	5.525699176680496	0.0
28	5.3741481975106946E-5	0.0	0.0	5.630763773941831	0.0
29	5.3741481975106946E-5	0.0	0.0	5.748027687611514	0.0
30	5.3741481975106946E-5	0.0	0.0	5.894956899331456	0.0
31	5.3741481975106946E-5	0.0	0.0	6.025817407940841	0.0
32	5.3741481975106946E-5	0.0	0.0	6.153077237257895	0.0
33	5.3741481975106946E-5	0.0	0.0	6.279853393237172	0.0
34	5.3741481975106946E-5	0.0	0.0	6.412487370751736	0.0
35	5.3741481975106946E-5	0.0	0.0	6.577151271523464	0.0
36	5.3741481975106946E-5	0.0	0.0	6.709838990520002	0.0
37	5.3741481975106946E-5	0.0	0.0	6.848706979943679	0.0
38	5.3741481975106946E-5	0.0	0.0	6.986016466390077	0.0
39	5.3741481975106946E-5	0.0	0.0	7.144123906360842	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGAC	45	3.8562575E-10	45.000004	9
TATACGA	45	3.8562575E-10	45.000004	22
ACGCATA	20	7.034414E-4	45.0	32
GTTACGT	25	3.8917402E-5	45.0	45
GTTACGA	25	3.8917402E-5	45.0	34
TCGCTAA	25	3.8917402E-5	45.0	15
CGTACGT	25	3.8917402E-5	45.0	27
ACGTCGT	20	7.034414E-4	45.0	31
CGTAATC	25	3.8917402E-5	45.0	34
TTGACGT	30	2.166229E-6	44.999996	12
CGTTTTT	7185	0.0	43.34029	1
CGTTAGG	395	0.0	41.582275	2
CTATGCG	60	3.6379788E-12	41.249996	1
CGGTCTA	235	0.0	41.17021	31
ACGTAAG	55	6.184564E-11	40.909092	1
CTAACGG	105	0.0	40.714287	2
CTATACG	50	1.0822987E-9	40.5	1
GGCGTAA	50	1.0822987E-9	40.5	8
TATACGG	370	0.0	40.135136	2
TGCAACG	40	3.4597542E-7	39.375	1
>>END_MODULE