FastQCFastQC Report
Thu 26 May 2016
SRR1546361_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546361_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2272986
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113480.4992551647920401No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC33930.14927500653325626No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG22950.10096850574530597No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG22820.10039657085437394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATA351.2126293E-745.014
TATAGCG1300.044.9999961
CGTTTTT85700.043.1096841
TCGTAAG1100.042.9545441
CGTAAGG4150.040.120482
TACGGGT3950.039.8734174
ACGTAAG1300.039.807691
ATAACGG1550.039.1935462
CTATACG750.039.0000041
TAACGGG5500.038.8636363
CGGTCTA2850.038.6842131
CCGTCTA356.250986E-638.57142619
AGGGTAA12850.038.52146
TAGGGTA16600.038.4939735
AGGGTAT15950.038.0877726
GGTCGAA659.094947E-1238.076928
TACGCGG1300.038.076922
TAGGGAC14600.038.0650675
GTAGGGT21500.037.6744164
ATAGGGT17250.037.1739124
Produced by FastQC (version 0.11.5)