##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546361_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2272986
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.183542705498407	31.0	31.0	33.0	30.0	34.0
2	31.529508320772763	31.0	31.0	34.0	30.0	34.0
3	31.638319373722496	31.0	31.0	34.0	30.0	34.0
4	35.50654161530252	37.0	35.0	37.0	33.0	37.0
5	35.43933442616893	37.0	35.0	37.0	33.0	37.0
6	35.48059028960143	37.0	35.0	37.0	33.0	37.0
7	35.83755553267816	37.0	35.0	37.0	35.0	37.0
8	35.73786728118871	37.0	35.0	37.0	35.0	37.0
9	37.52230502079643	39.0	37.0	39.0	35.0	39.0
10	36.83549260752156	39.0	37.0	39.0	32.0	39.0
11	36.70232768701611	39.0	35.0	39.0	32.0	39.0
12	36.2897215381001	38.0	35.0	39.0	32.0	39.0
13	36.122017469531265	38.0	35.0	39.0	32.0	39.0
14	37.083133816046384	39.0	35.0	41.0	31.0	41.0
15	37.263910996372175	39.0	35.0	41.0	32.0	41.0
16	37.281552988007846	39.0	35.0	41.0	32.0	41.0
17	37.207859177311256	39.0	35.0	41.0	32.0	41.0
18	37.111742439240714	39.0	35.0	41.0	32.0	41.0
19	37.03189020961854	38.0	35.0	41.0	32.0	41.0
20	36.88717132441643	38.0	35.0	41.0	31.0	41.0
21	36.77064398988819	38.0	35.0	41.0	31.0	41.0
22	36.68292853541553	38.0	35.0	40.0	31.0	41.0
23	36.595807893229434	38.0	35.0	40.0	31.0	41.0
24	36.60558929971412	38.0	35.0	40.0	31.0	41.0
25	36.55064835419136	38.0	35.0	40.0	31.0	41.0
26	36.48038527294053	38.0	35.0	40.0	31.0	41.0
27	36.376418948466906	38.0	35.0	40.0	30.0	41.0
28	36.307603742389965	38.0	35.0	40.0	30.0	41.0
29	36.21051779465426	38.0	35.0	40.0	30.0	41.0
30	36.04702668648201	38.0	35.0	40.0	30.0	41.0
31	35.865568463686095	38.0	34.0	40.0	29.0	41.0
32	35.712651111797435	38.0	34.0	40.0	29.0	41.0
33	35.53641905405489	38.0	34.0	40.0	28.0	41.0
34	35.28789662584811	38.0	34.0	40.0	26.0	41.0
35	35.08459972916683	38.0	34.0	40.0	26.0	41.0
36	34.90047540987934	38.0	34.0	40.0	25.0	41.0
37	34.74548325418635	37.0	33.0	40.0	24.0	41.0
38	34.63698588552679	37.0	33.0	40.0	24.0	41.0
39	34.55696603498658	37.0	33.0	40.0	24.0	41.0
40	34.36751876166417	37.0	33.0	40.0	23.0	41.0
41	34.250649586051125	37.0	33.0	40.0	23.0	41.0
42	34.14947650359483	37.0	33.0	40.0	23.0	41.0
43	34.12408171453762	36.0	33.0	40.0	23.0	41.0
44	33.95392580508635	36.0	33.0	40.0	23.0	41.0
45	33.893637708283286	36.0	33.0	40.0	23.0	41.0
46	33.81341504083175	36.0	33.0	40.0	23.0	41.0
47	33.750856802461605	35.0	33.0	40.0	23.0	41.0
48	33.64792392034091	35.0	33.0	40.0	23.0	41.0
49	33.62184808881357	35.0	32.0	39.0	23.0	41.0
50	33.50997146484844	35.0	32.0	39.0	22.0	41.0
51	33.319254056118254	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	4.0
12	8.0
13	14.0
14	50.0
15	102.0
16	248.0
17	558.0
18	1297.0
19	2399.0
20	4267.0
21	6670.0
22	10043.0
23	14653.0
24	21074.0
25	29122.0
26	38655.0
27	44430.0
28	48405.0
29	53994.0
30	63149.0
31	76370.0
32	94409.0
33	120501.0
34	190405.0
35	239360.0
36	187051.0
37	244932.0
38	339411.0
39	441337.0
40	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.39892986582407	23.503092407960278	28.567971822087774	14.530005904127874
2	32.00516853161436	24.65237357379236	30.006564052748235	13.335893841845046
3	29.688568253390034	24.84467568212035	32.264167047223346	13.202589017266275
4	25.83883050753502	27.540952737940312	31.02887567279341	15.591341081731255
5	22.638898787762002	32.18022460323117	30.359931825361002	14.820944783645828
6	20.13184419085731	42.146981987570534	26.753970328017857	10.967203493554294
7	88.74700504094614	3.2938170318690925	6.409586332691887	1.5495915944928829
8	90.13759873575992	2.3393896838783874	6.096781942343683	1.4262296380180082
9	85.89023425573232	3.3987450868593116	8.04980760990169	2.661213047506672
10	40.96699231759457	21.324504418417007	23.839214143861863	13.869289120126565
11	37.38958356980641	23.45878945140885	21.51253901255881	17.639087966225926
12	32.290388062222995	21.46075690743366	27.6153922637447	18.633462766598647
13	25.51850297362148	26.809404017446653	27.47755595503008	20.194537053901783
14	22.12257356622522	29.660103493818262	29.155480940049785	19.061841999906733
15	21.792699119132276	22.050685749934225	36.5269737693061	19.629641361627392
16	23.365960019111423	19.62607776730697	34.98970077246406	22.01826144111754
17	23.31369396907856	20.046889862058105	28.384028762165713	28.255387406697626
18	25.84705757096612	22.55332852908025	30.531600282623828	21.068013617329804
19	28.307477476764042	24.97564877214378	25.502400806692165	21.214472944400008
20	29.172111046878424	24.25017136049232	25.010932755415123	21.566784837214133
21	26.6631646653345	25.11242920105975	27.579976295498522	20.644429838107232
22	25.813533387359183	21.39098085073995	27.31341064133259	25.482075120568275
23	23.99975186824732	26.562768094480127	25.944242507432953	23.493237529839604
24	23.632481678285743	25.037945680263757	30.62962112393125	20.699951517519246
25	23.607492523051178	23.603928928730753	28.332906581914713	24.455671966303356
26	22.3945506043592	25.52585013722038	26.50693845012684	25.572660808293584
27	21.46379256185476	25.274418760168345	29.383902936489708	23.87788574148719
28	20.621244477528677	25.518151013688602	31.988890384718605	21.871714124064116
29	21.724990827044248	25.50662432588674	30.471635109059186	22.296749738009826
30	23.515059045678242	24.49966695791351	30.708152183955377	21.27712181245287
31	27.990273587254826	23.577135978840168	26.052030236877833	22.380560197027172
32	26.987539738476173	24.75171426484809	26.72585752837897	21.53488846829677
33	26.49585171224108	24.54190214985926	26.425679700622883	22.536566437276782
34	22.095736621343026	26.395719111336362	28.805676761757443	22.70286750556317
35	23.260636009196713	25.647012344114746	28.812451990465405	22.279899656223133
36	27.47126467122983	26.38181669398756	24.810975518546968	21.335943116235647
37	24.010530641191806	27.72471981789593	26.166021260139743	22.098728280772516
38	24.6269884636333	28.01020331845423	23.581931432925675	23.780876784986795
39	23.332919780412197	26.724097728714565	24.840804122858653	25.102178368014584
40	24.68932936674489	23.761211023737058	26.494531862492778	25.05492774702528
41	22.155349834974785	23.726498975356645	27.284373946869888	26.83377724279868
42	23.719987716598343	25.341291147415777	25.27340687536131	25.665314260624573
43	23.219148732108337	25.786168502577667	25.890348642710514	25.104334122603483
44	23.40986702073836	25.982430160150567	27.01750032776269	23.590202491348386
45	22.971104969410284	27.27284725906803	25.797871170345964	23.958176601175722
46	23.61096812738838	26.027393041576147	27.23127199199643	23.130366839039045
47	23.981317966762663	26.20165720334397	27.157580381049424	22.659444448843942
48	23.690291097261486	25.768350530975553	27.403028439242476	23.13832993252048
49	22.689009083205967	24.519288724171638	28.60303583040107	24.188666362221326
50	22.622928605807516	25.960916609253204	28.10734425992945	23.308810525009832
51	22.943476114679104	27.090268043885885	26.11397518506493	23.852280656370077
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	688.0
1	1620.0
2	2552.0
3	12193.5
4	21835.0
5	15795.5
6	9756.0
7	9726.5
8	9697.0
9	9583.5
10	9470.0
11	9415.5
12	9361.0
13	8928.0
14	8495.0
15	8016.0
16	7537.0
17	6981.5
18	6426.0
19	6045.5
20	5665.0
21	6256.5
22	6848.0
23	6875.0
24	6902.0
25	7644.5
26	10147.0
27	11907.0
28	14566.5
29	17226.0
30	20545.0
31	23864.0
32	28533.0
33	33202.0
34	40939.5
35	48677.0
36	53162.0
37	57647.0
38	63487.5
39	69328.0
40	85271.0
41	101214.0
42	116452.5
43	131691.0
44	136713.0
45	141735.0
46	154372.5
47	167010.0
48	173679.5
49	180349.0
50	178039.0
51	175729.0
52	170390.0
53	165051.0
54	157921.0
55	150791.0
56	144914.5
57	139038.0
58	135636.5
59	132235.0
60	122389.5
61	112544.0
62	102953.0
63	93362.0
64	84441.5
65	75521.0
66	63129.5
67	50738.0
68	40750.5
69	30763.0
70	25153.0
71	19543.0
72	16292.5
73	13042.0
74	10554.5
75	5991.0
76	3915.0
77	3260.0
78	2605.0
79	1879.0
80	1153.0
81	962.0
82	771.0
83	581.0
84	391.0
85	260.0
86	129.0
87	95.0
88	61.0
89	51.0
90	41.0
91	26.5
92	12.0
93	9.0
94	6.0
95	6.0
96	6.0
97	4.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2272986.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.65436845463369
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.04068202188456	15.338403189090263
2	6.445091080511636	2.5334838964009774
3	2.435979357567979	1.4363290752456876
4	1.4717942587524875	1.1570874660374344
5	0.9792618889376332	0.9623386989380409
6	0.7827408613313396	0.9230566375862089
7	0.6353466644409648	0.8741136207541663
8	0.538034542154278	0.8459783306256259
9	0.4533907661269025	0.8019998254248113
>10	5.5208146155152065	25.656854025501914
>50	1.8966788952789766	26.94760915076111
>100	0.7907340454088594	20.156467917596906
>500	0.005850620340902239	0.7511074536718388
>1k	0.003375357888982061	1.1132825074384696
>5k	0.0	0.0
>10k+	2.250238592654707E-4	0.5018882049265753
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11348	0.4992551647920401	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3393	0.14927500653325626	No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	2295	0.10096850574530597	No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	2282	0.10039657085437394	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.023581315503043133	0.0
2	0.0	0.0	0.0	0.07773915017514406	0.0
3	0.0	0.0	0.0	0.11231921358072597	0.0
4	0.0	0.0	0.0	0.15653418014893183	0.0
5	0.0	0.0	0.0	0.25816261076839014	0.0
6	0.0	0.0	0.0	0.391159470405889	0.0
7	0.0	0.0	0.0	0.4916880262350934	0.0
8	0.0	0.0	0.0	0.8133794048885475	0.0
9	0.0	0.0	0.0	0.9999621643072153	0.0
10	0.0	0.0	0.0	1.3203776882039748	0.0
11	0.0	0.0	0.0	1.5619101921437264	0.0
12	0.0	0.0	0.0	1.7641111735839992	0.0
13	0.0	0.0	0.0	1.850033392198632	0.0
14	0.0	0.0	0.0	1.882369711032096	0.0
15	0.0	0.0	0.0	1.9282124922898778	0.0
16	0.0	0.0	0.0	2.022977704218152	0.0
17	0.0	0.0	0.0	2.130897418637862	0.0
18	0.0	0.0	0.0	2.2938108725702664	0.0
19	0.0	0.0	0.0	2.36279501941499	0.0
20	0.0	0.0	0.0	2.4509169876101304	0.0
21	0.0	0.0	0.0	2.5502136836742504	0.0
22	0.0	0.0	0.0	2.649598369721591	0.0
23	0.0	0.0	0.0	2.761697608344266	0.0
24	0.0	0.0	0.0	2.8443641975797473	0.0
25	0.0	0.0	0.0	2.920167568124045	0.0
26	0.0	0.0	0.0	2.9932432491885126	0.0
27	0.0	0.0	0.0	3.0776696380884	0.0
28	0.0	0.0	0.0	3.158488437676255	0.0
29	0.0	0.0	0.0	3.2486341754854626	0.0
30	0.0	0.0	0.0	3.3615253239571206	0.0
31	0.0	0.0	0.0	3.4513630968250575	0.0
32	0.0	0.0	0.0	3.5427846893909596	0.0
33	0.0	0.0	0.0	3.6436212101614354	0.0
34	0.0	0.0	0.0	3.742038006393352	0.0
35	0.0	0.0	0.0	3.855237119806281	0.0
36	0.0	0.0	0.0	3.952114091331843	0.0
37	0.0	0.0	0.0	4.056778176372402	0.0
38	0.0	0.0	0.0	4.188059231337105	0.0
39	0.0	0.0	0.0	4.400247075872882	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTATA	35	1.2126293E-7	45.0	14
TATAGCG	130	0.0	44.999996	1
CGTTTTT	8570	0.0	43.109684	1
TCGTAAG	110	0.0	42.954544	1
CGTAAGG	415	0.0	40.12048	2
TACGGGT	395	0.0	39.873417	4
ACGTAAG	130	0.0	39.80769	1
ATAACGG	155	0.0	39.193546	2
CTATACG	75	0.0	39.000004	1
TAACGGG	550	0.0	38.863636	3
CGGTCTA	285	0.0	38.68421	31
CCGTCTA	35	6.250986E-6	38.571426	19
AGGGTAA	1285	0.0	38.5214	6
TAGGGTA	1660	0.0	38.493973	5
AGGGTAT	1595	0.0	38.087772	6
GGTCGAA	65	9.094947E-12	38.07692	8
TACGCGG	130	0.0	38.07692	2
TAGGGAC	1460	0.0	38.065067	5
GTAGGGT	2150	0.0	37.674416	4
ATAGGGT	1725	0.0	37.173912	4
>>END_MODULE