FastQCFastQC Report
Thu 26 May 2016
SRR1546360_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546360_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1743055
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC94790.5438153127698209No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87160.5000415936387549No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAC207.03429E-445.00000432
TTCTACG207.03429E-445.0000041
GGTACGA207.03429E-445.0000042
AGTTTCG207.03429E-445.0000041
ACGTTCC406.8193913E-945.00000410
GCGAAGT207.03429E-445.00000413
GTTTAAC207.03429E-445.00000442
CACGACC800.045.00000427
TCGATAT207.03429E-445.00000442
TTATCCG207.03429E-445.00000438
CGGATAT207.03429E-445.0000042
TCTAACG406.8193913E-945.0000041
TATAGCG2300.045.0000041
CGTCGGA207.03429E-445.00000418
CGTCGAG207.03429E-445.00000426
TCGAGTG406.8193913E-945.00000431
CGCGTGT207.03429E-445.00000420
CGCAATC207.03429E-445.00000422
GCTAACC207.03429E-445.00000440
TTCGCGA406.8193913E-945.00000416