##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546360_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1743055
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.268497551712368	31.0	31.0	33.0	30.0	34.0
2	31.603803092845606	31.0	31.0	34.0	30.0	34.0
3	31.658749723904293	31.0	31.0	34.0	30.0	34.0
4	35.50942282372042	37.0	35.0	37.0	33.0	37.0
5	35.42206356081707	37.0	35.0	37.0	33.0	37.0
6	35.47756037531805	37.0	35.0	37.0	33.0	37.0
7	35.852060893087135	37.0	35.0	37.0	35.0	37.0
8	35.76667861886171	37.0	35.0	37.0	35.0	37.0
9	37.54667982364297	39.0	37.0	39.0	35.0	39.0
10	37.11142677655037	39.0	37.0	39.0	33.0	39.0
11	36.77908786584474	39.0	35.0	39.0	32.0	39.0
12	35.669723560071255	37.0	35.0	39.0	31.0	39.0
13	35.17255164065391	37.0	35.0	39.0	30.0	39.0
14	36.01686579023611	38.0	35.0	40.0	30.0	41.0
15	36.414130936774804	38.0	35.0	40.0	31.0	41.0
16	36.58339008235541	38.0	35.0	40.0	32.0	41.0
17	36.534984266130444	38.0	35.0	40.0	32.0	41.0
18	36.46732145571998	37.0	35.0	40.0	31.0	41.0
19	36.33174340453973	37.0	35.0	40.0	31.0	41.0
20	36.15299173003721	36.0	35.0	40.0	31.0	41.0
21	35.98898313593088	36.0	34.0	40.0	31.0	41.0
22	35.875111227127086	36.0	34.0	40.0	31.0	41.0
23	35.86033372440915	35.0	34.0	40.0	31.0	41.0
24	35.83256179523882	35.0	34.0	40.0	31.0	41.0
25	35.749446230899196	35.0	34.0	40.0	31.0	41.0
26	35.65112862187367	35.0	34.0	40.0	30.0	41.0
27	35.50744927727467	35.0	34.0	40.0	30.0	41.0
28	35.49087435565716	36.0	34.0	40.0	30.0	41.0
29	35.4976544056269	36.0	34.0	40.0	30.0	41.0
30	35.38232069556038	36.0	34.0	40.0	30.0	41.0
31	35.13389479964774	35.0	34.0	40.0	29.0	41.0
32	34.887775772996264	35.0	34.0	40.0	29.0	41.0
33	34.676085952537356	35.0	34.0	40.0	27.0	41.0
34	34.448752334263695	35.0	34.0	40.0	25.0	41.0
35	34.26832371898764	35.0	33.0	40.0	24.0	41.0
36	34.036637971836804	35.0	33.0	40.0	23.0	41.0
37	33.85948119824102	35.0	33.0	40.0	23.0	41.0
38	33.84268195782692	35.0	33.0	40.0	23.0	41.0
39	33.816547383760124	35.0	33.0	40.0	23.0	41.0
40	33.560486043182806	35.0	33.0	39.0	23.0	41.0
41	33.53077900582598	35.0	33.0	39.0	23.0	41.0
42	33.42154378375898	35.0	33.0	39.0	23.0	41.0
43	33.34069607671588	35.0	33.0	39.0	23.0	41.0
44	33.14025317617631	35.0	32.0	39.0	22.0	41.0
45	33.105496957927315	35.0	32.0	39.0	23.0	41.0
46	33.044431759181435	35.0	32.0	39.0	22.0	41.0
47	33.01261578091339	35.0	32.0	39.0	22.0	41.0
48	32.965367702109226	35.0	32.0	38.0	22.0	41.0
49	33.012680609619316	35.0	32.0	38.0	22.0	40.0
50	32.834128584582814	35.0	32.0	38.0	21.0	40.0
51	32.60129714782379	35.0	32.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	6.0
12	8.0
13	7.0
14	22.0
15	64.0
16	171.0
17	433.0
18	1093.0
19	2225.0
20	3693.0
21	5864.0
22	8675.0
23	12819.0
24	18322.0
25	26174.0
26	33754.0
27	38771.0
28	41451.0
29	45447.0
30	52636.0
31	64235.0
32	80870.0
33	107435.0
34	199311.0
35	287134.0
36	115377.0
37	140131.0
38	192517.0
39	264344.0
40	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.58258918966986	22.89652363235813	28.623135816138905	14.897751361833103
2	31.35317015240483	24.909254154343955	30.525657538058184	13.211918155193036
3	28.688194004205258	24.9709274807737	32.1870508962712	14.15382761874984
4	25.959192337591187	26.76083084010545	31.73537266466061	15.54460415764276
5	22.40273542716667	32.87624314780658	30.54309818106715	14.1779232439596
6	20.07177054080336	42.214043733559755	27.416289216347163	10.297896509289723
7	85.63407350886804	5.324788948139904	7.472971306126312	1.568166236865733
8	87.44933464520625	3.754614742506691	6.922443640619487	1.8736069716675605
9	83.88226418558222	4.859513899446661	8.48039792203918	2.77782399293195
10	56.45243552268862	19.63707398791203	12.64762156099492	11.262868928404439
11	51.19729440551216	18.169535671565153	17.79702877992949	12.836141142993194
12	45.038968936723165	19.60311063047351	23.06984002225977	12.288080410543557
13	22.70094747440557	40.794237703342695	23.649282438018307	12.85553238423343
14	16.032597938676634	43.45864014618013	27.430058145038455	13.078703770104788
15	14.387842035965589	20.820570779464788	50.998849720748915	13.792737463820707
16	15.638519725424613	15.172670971369234	49.42253686774084	19.766272435465318
17	15.929962049390294	16.28439722211864	29.340726483100077	38.44491424539099
18	22.935650338055886	21.425084119548725	34.10219413615749	21.5370714062379
19	30.807060018186462	23.560759700640542	25.329493332109426	20.30268694906357
20	32.73258732512743	21.861157565309185	23.219519751241354	22.186735358322025
21	23.861094457719346	27.19065089741861	27.058067588228713	21.890187056633327
22	23.30758352432941	23.872740676570732	24.835934609062825	27.98374119003703
23	20.38220251225578	29.948509943748192	23.99941482053062	25.66987272346541
24	22.54231794177464	22.885508489405098	37.58475779593874	16.98741577288152
25	18.262361199158946	23.009945182452647	35.74293410133358	22.984759517054826
26	17.745510038409574	32.548427903881404	27.14813933008425	22.557922727624774
27	19.02200446916477	31.429300853960427	29.163681008344543	20.385013668530252
28	15.343864651430964	27.704920384038367	39.34580377555499	17.60541118897568
29	17.49629242909719	23.579519865982427	37.80924870414301	21.114939000777373
30	19.571786317700816	28.201978709794012	32.460708354010634	19.76552661849454
31	31.746789401367142	24.97775457458313	23.432020217376962	19.84343580667277
32	31.872258764066537	26.118969280946384	24.883724265728848	17.125047689258228
33	29.757867651910008	25.71140899168414	24.904320288229574	19.626403068176277
34	20.88092458356162	26.93099185051533	28.448327792295714	23.739755773627337
35	20.971340548634437	24.789005510440003	31.15283223994653	23.08682170097903
36	31.33515580403372	23.70286651884192	25.817659224751942	19.144318452372417
37	22.094540906626584	31.945234086130387	27.29156567061854	18.66865933662449
38	22.081288312761217	32.193419025790924	22.11439111215653	23.61090154929133
39	22.053176750016494	30.08665819495082	25.66866794220492	22.191497112827765
40	23.65983861668163	25.844508635699963	24.781317858587364	25.714334889031043
41	17.85055549021689	23.3966225965331	26.25143784906386	32.501384064186155
42	22.924692565639067	25.315150698055998	23.31458272974748	28.44557400655745
43	23.580208312416993	25.52122566413567	24.14439016554268	26.754175857904656
44	21.160892800284557	29.423626907928895	26.977003020558733	22.438477271227818
45	19.16967622937888	34.55215125168167	22.559356991030118	23.71881552790933
46	23.113269518173553	31.700663490251312	25.434424042844316	19.751642948730822
47	23.42903694949385	26.404789292363123	26.975798239298243	23.190375518844785
48	23.505798726947802	22.22442780061444	29.44978787244235	24.819985599995412
49	21.432943883010005	21.699200541577863	32.244937767310844	24.622917808101295
50	20.277788136346818	30.673444039344716	26.63983637923072	22.40893144507775
51	18.777720725966766	33.49452541658181	23.712676880534463	24.015076976916966
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	909.0
1	1400.0
2	1891.0
3	9035.0
4	16179.0
5	11956.5
6	7734.0
7	8204.0
8	8674.0
9	9177.5
10	9681.0
11	9728.5
12	9776.0
13	9368.0
14	8960.0
15	8469.5
16	7979.0
17	7499.0
18	7019.0
19	6705.5
20	6392.0
21	6047.5
22	5703.0
23	5671.0
24	5639.0
25	6033.5
26	7120.5
27	7813.0
28	10170.0
29	12527.0
30	14940.0
31	17353.0
32	20623.5
33	23894.0
34	26361.5
35	28829.0
36	30126.0
37	31423.0
38	41117.5
39	50812.0
40	79140.5
41	107469.0
42	136541.5
43	165614.0
44	168880.0
45	172146.0
46	163446.0
47	154746.0
48	151042.0
49	147338.0
50	139195.5
51	131053.0
52	120239.5
53	109426.0
54	100381.0
55	91336.0
56	87592.5
57	83849.0
58	77905.5
59	71962.0
60	69807.5
61	67653.0
62	60717.5
63	53782.0
64	47908.0
65	42034.0
66	33375.5
67	24717.0
68	20583.5
69	16450.0
70	13711.0
71	10972.0
72	9378.0
73	7784.0
74	6516.5
75	3662.5
76	2076.0
77	1536.0
78	996.0
79	724.5
80	453.0
81	327.0
82	201.0
83	133.0
84	65.0
85	49.0
86	33.0
87	22.0
88	11.0
89	10.0
90	9.0
91	7.0
92	5.0
93	7.0
94	9.0
95	5.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1743055.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.42579034581574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.62975812321626	13.572590832574155
2	7.036026381892427	2.31144588433187
3	2.262342310915837	1.1148228146871548
4	1.0603968940172057	0.6967142825792326
5	0.6471510376948199	0.5314983633626106
6	0.47337088956109313	0.4665294592645688
7	0.32355750091997654	0.37202813724493333
8	0.2641806087851785	0.34715002346682483
9	0.2349962241777444	0.34739988393617516
>10	2.4803169781781276	9.30807937040945
>50	0.8647409091508853	10.383837370793843
>100	1.6992878493916619	56.79141315797781
>500	0.020714459321096702	2.1886813288093396
>1k	0.0024576477160623204	0.518508479851517
>5k	7.021850617320916E-4	1.0493006107105276
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	9479	0.5438153127698209	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8716	0.5000415936387549	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06178806750217291	0.0
2	0.0	0.0	0.0	0.21777855546726868	0.0
3	0.0	0.0	0.0	0.30612918123639243	0.0
4	0.0	0.0	0.0	0.43033639213908914	0.0
5	0.0	0.0	0.0	0.6424352645211998	0.0
6	0.0	0.0	0.0	0.87042577543451	0.0
7	0.0	0.0	0.0	1.0110983302305434	0.0
8	0.0	0.0	0.0	1.4730458878233905	0.0
9	0.0	0.0	0.0	1.6765965503096574	0.0
10	0.0	0.0	0.0	1.9979862941788986	0.0
11	0.0	0.0	0.0	2.2204118630794785	0.0
12	0.0	0.0	0.0	2.423331449667394	0.0
13	0.0	0.0	0.0	2.5045107584098036	0.0
14	0.0	0.0	0.0	2.53440080777715	0.0
15	0.0	0.0	0.0	2.586034290369495	0.0
16	0.0	0.0	0.0	2.6999148047537225	0.0
17	0.0	0.0	0.0	2.829859069277791	0.0
18	0.0	0.0	0.0	3.0612918123639243	0.0
19	0.0	0.0	0.0	3.1414958219907003	0.0
20	0.0	0.0	0.0	3.24969665328977	0.0
21	0.0	0.0	0.0	3.3530209890106737	0.0
22	0.0	0.0	0.0	3.451640940762053	0.0
23	0.0	0.0	0.0	3.565636196218708	0.0
24	0.0	0.0	0.0	3.6485366210475285	0.0
25	5.737053621371672E-5	0.0	0.0	3.71061154123077	0.0
26	5.737053621371672E-5	0.0	0.0	3.780718336483932	0.0
27	5.737053621371672E-5	0.0	0.0	3.857078520184389	0.0
28	5.737053621371672E-5	0.0	0.0	3.9298817306395954	0.0
29	5.737053621371672E-5	0.0	0.0	4.004922392007137	0.0
30	5.737053621371672E-5	0.0	0.0	4.106869834858911	0.0
31	5.737053621371672E-5	0.0	0.0	4.205489786610291	0.0
32	5.737053621371672E-5	0.0	0.0	4.289537622163386	0.0
33	5.737053621371672E-5	0.0	0.0	4.376683466672021	0.0
34	5.737053621371672E-5	0.0	0.0	4.471057998743586	0.0
35	5.737053621371672E-5	0.0	0.0	4.592224571226955	0.0
36	1.1474107242743344E-4	0.0	0.0	4.691475598876685	0.0
37	1.1474107242743344E-4	0.0	0.0	4.785735389875821	0.0
38	1.1474107242743344E-4	0.0	0.0	4.883953747873704	0.0
39	1.1474107242743344E-4	0.0	0.0	4.991179280057141	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAC	20	7.03429E-4	45.000004	32
TTCTACG	20	7.03429E-4	45.000004	1
GGTACGA	20	7.03429E-4	45.000004	2
AGTTTCG	20	7.03429E-4	45.000004	1
ACGTTCC	40	6.8193913E-9	45.000004	10
GCGAAGT	20	7.03429E-4	45.000004	13
GTTTAAC	20	7.03429E-4	45.000004	42
CACGACC	80	0.0	45.000004	27
TCGATAT	20	7.03429E-4	45.000004	42
TTATCCG	20	7.03429E-4	45.000004	38
CGGATAT	20	7.03429E-4	45.000004	2
TCTAACG	40	6.8193913E-9	45.000004	1
TATAGCG	230	0.0	45.000004	1
CGTCGGA	20	7.03429E-4	45.000004	18
CGTCGAG	20	7.03429E-4	45.000004	26
TCGAGTG	40	6.8193913E-9	45.000004	31
CGCGTGT	20	7.03429E-4	45.000004	20
CGCAATC	20	7.03429E-4	45.000004	22
GCTAACC	20	7.03429E-4	45.000004	40
TTCGCGA	40	6.8193913E-9	45.000004	16
>>END_MODULE