##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546359_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2055644
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.131042631895404	31.0	31.0	33.0	30.0	34.0
2	31.469733086079106	31.0	31.0	34.0	30.0	34.0
3	31.51228325527183	31.0	31.0	34.0	30.0	34.0
4	35.360079858185564	37.0	35.0	37.0	33.0	37.0
5	35.26120086941124	37.0	35.0	37.0	33.0	37.0
6	35.296150014302086	37.0	35.0	37.0	32.0	37.0
7	35.76218109750521	37.0	35.0	37.0	35.0	37.0
8	35.677246643874135	37.0	35.0	37.0	35.0	37.0
9	37.45047148241622	39.0	37.0	39.0	35.0	39.0
10	36.98699531630963	39.0	37.0	39.0	33.0	39.0
11	36.62060454047491	39.0	35.0	39.0	32.0	39.0
12	35.42048623205185	37.0	35.0	39.0	31.0	39.0
13	34.818812012196666	37.0	34.0	39.0	28.0	39.0
14	35.60277898313132	37.0	34.0	40.0	27.0	41.0
15	36.090374111470666	37.0	35.0	40.0	31.0	41.0
16	36.3314849263783	37.0	35.0	40.0	32.0	41.0
17	36.295560904514595	37.0	35.0	40.0	31.0	41.0
18	36.22272387631321	36.0	35.0	40.0	31.0	41.0
19	36.046020127998815	36.0	35.0	40.0	31.0	41.0
20	35.850214823189226	36.0	34.0	40.0	31.0	41.0
21	35.65113414579567	35.0	34.0	40.0	30.0	41.0
22	35.518438990408846	35.0	34.0	40.0	30.0	41.0
23	35.50296889928412	35.0	34.0	40.0	30.0	41.0
24	35.47142258095273	35.0	34.0	40.0	30.0	41.0
25	35.41171477162388	35.0	34.0	40.0	30.0	41.0
26	35.26933505996175	35.0	34.0	40.0	30.0	41.0
27	35.15101058354462	35.0	34.0	40.0	29.0	41.0
28	35.14115479139384	35.0	34.0	40.0	29.0	41.0
29	35.13736327885568	35.0	34.0	40.0	29.0	41.0
30	35.009274465812176	36.0	34.0	40.0	29.0	41.0
31	34.70473438007748	35.0	34.0	40.0	27.0	41.0
32	34.4196582676767	35.0	34.0	40.0	26.0	41.0
33	34.184672540576095	35.0	33.0	40.0	25.0	41.0
34	33.96348540895213	35.0	33.0	39.0	23.0	41.0
35	33.768562552659894	35.0	33.0	39.0	23.0	41.0
36	33.50691024321332	35.0	33.0	39.0	22.0	41.0
37	33.29597002204662	35.0	33.0	39.0	22.0	41.0
38	33.256689874316756	35.0	32.0	39.0	23.0	41.0
39	33.2297912479009	35.0	32.0	39.0	22.0	41.0
40	32.95374199034463	35.0	32.0	39.0	21.0	41.0
41	32.90330232277574	35.0	32.0	39.0	20.0	41.0
42	32.84988354014606	35.0	32.0	39.0	20.0	41.0
43	32.73209612170201	35.0	32.0	38.0	20.0	41.0
44	32.52538620500437	35.0	31.0	38.0	18.0	41.0
45	32.51131421588563	35.0	31.0	38.0	18.0	40.0
46	32.461024866173325	35.0	31.0	38.0	18.0	40.0
47	32.42327757140828	35.0	31.0	38.0	18.0	40.0
48	32.4081499520345	35.0	31.0	38.0	18.0	40.0
49	32.42069103405064	35.0	31.0	38.0	18.0	40.0
50	32.22056153691982	35.0	31.0	38.0	18.0	40.0
51	31.97463276715229	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	2.0
13	4.0
14	37.0
15	111.0
16	279.0
17	767.0
18	1716.0
19	3277.0
20	5679.0
21	9067.0
22	13093.0
23	18977.0
24	26302.0
25	36110.0
26	46015.0
27	51858.0
28	54401.0
29	59032.0
30	67896.0
31	81716.0
32	101384.0
33	132195.0
34	240889.0
35	346975.0
36	129529.0
37	151373.0
38	202720.0
39	274187.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.91097291165202	23.894847551424274	28.980212527071807	15.2139670098519
2	30.790399504972648	25.92087929622055	30.599218541731933	12.689502657074863
3	27.577197218973716	26.273323591049813	32.555101953451086	13.59437723652539
4	25.1157301556106	28.06594916240361	32.14165487798471	14.676665804001082
5	23.102492454919236	33.050226595655666	30.527124346433528	13.320156602991567
6	19.620858475494785	40.43564936341117	28.667512468112182	11.27597969298186
7	84.76842293704551	5.161740067832757	8.201955202359942	1.8678817927617817
8	85.83324739108524	4.235704236725814	7.288470182580252	2.6425781896087064
9	82.76233628001735	4.860180070089958	9.408049253664545	2.9694343962281406
10	58.04852396621205	18.35108608299881	12.665130732753338	10.935259218035807
11	51.89736160541417	17.703065316757183	19.39547898371508	11.004094094113572
12	46.05281848413441	19.864675011821113	22.967303677095842	11.115202826948636
13	21.785678843223828	43.83784351765188	23.890177482093204	10.486300157031081
14	12.67208719019441	46.79312176622022	28.22497475243768	12.309816291147689
15	11.128629276275465	21.211260315502102	55.615612430946214	12.04449797727622
16	12.771520749701798	14.609241678033747	52.55452792409581	20.06470964816865
17	13.739635851343909	15.623473714320186	30.38998970638885	40.24690072794706
18	21.267933552696867	20.500096320179953	35.54263286833713	22.689337258786054
19	28.569635598381822	23.136690983458223	25.9443755825425	22.349297835617453
20	31.431025994773414	20.10552410825999	24.85688183362489	23.606568063341708
21	21.10380007433194	28.293566395737784	27.66320432915427	22.93942920077601
22	21.65535472095363	24.23590855225905	24.85235770396041	29.256379022826913
23	17.259165497527782	31.56553372081936	23.840557995450574	27.33474278620228
24	19.76144702098223	22.671289386683686	39.43007641400943	18.137187178324652
25	14.935319539764668	22.93670499366622	37.80820025257292	24.3197752139962
26	14.468020727324383	33.752099098871206	29.368509333328145	22.41137084047627
27	16.00486270969098	34.37385072512556	29.495136317377913	20.126150247805555
28	13.352895734864598	28.381665307806216	40.75666798336677	17.508770973962417
29	13.541011965106797	23.91017121641685	39.96188055908513	22.58693625939122
30	17.731523551743393	29.126979185111818	32.712035741597276	20.429461521547505
31	30.11260704674545	25.3906804874774	23.53004703148989	20.96666543428726
32	30.466656677907267	26.36764926222634	24.752340385786646	18.41335367407975
33	27.494060255569547	26.025663976836455	25.08172621329374	21.39854955430026
34	19.309034054534735	27.012362062691793	27.92477685824977	25.7538270245237
35	19.598578353061132	23.977498049273123	31.715121879080233	24.708801718585512
36	30.979050847325706	22.217757549458952	27.29164193800094	19.511549665214407
37	20.79455392081508	30.349369832519642	28.32090575994676	20.535170486718517
38	20.849135356121977	32.62189367419651	22.229140843453436	24.299830126228084
39	20.44011511720901	29.74790381992213	27.377551755070428	22.434429307798432
40	25.03278777842856	23.77118800726196	24.72363891802277	26.47238529628671
41	16.62320907705809	22.776414593188317	26.927619762955064	33.67275656679853
42	22.72426548565802	24.30459748867022	23.707558312626116	29.263578713045646
43	23.38191826989498	25.013961561437682	25.528982644854846	26.07513752381249
44	20.130771670581094	30.22974795246648	27.453099855811608	22.18638052114082
45	16.930071549353876	35.814664406872005	23.132020914127153	24.123243129646962
46	22.85191404737396	31.08242477783118	26.220347492075476	19.845313682719382
47	22.38544222637772	25.767788585961384	26.750059835263308	25.096709352397596
48	24.619000177073463	21.545267565784737	29.716478145048463	24.119254112093337
49	21.558645368556036	20.396430510341286	33.19850129691717	24.84642282418551
50	19.47992940411861	31.259449593412086	26.40924206720619	22.85137893526311
51	18.352496833109235	34.02773048251546	22.49397269176959	25.125799992605724
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1542.0
1	1932.0
2	2322.0
3	9057.5
4	15793.0
5	12603.5
6	9414.0
7	10370.5
8	11327.0
9	12115.5
10	12904.0
11	13061.5
12	13219.0
13	12770.5
14	12322.0
15	12021.5
16	11721.0
17	11049.5
18	10378.0
19	9946.0
20	9514.0
21	8977.5
22	8441.0
23	7968.0
24	7495.0
25	7799.5
26	7913.0
27	7722.0
28	8591.0
29	9460.0
30	12934.5
31	16409.0
32	18709.0
33	21009.0
34	24096.0
35	27183.0
36	30191.0
37	33199.0
38	48372.0
39	63545.0
40	99462.0
41	135379.0
42	179367.0
43	223355.0
44	227732.0
45	232109.0
46	226070.5
47	220032.0
48	203507.0
49	186982.0
50	172764.0
51	158546.0
52	144719.0
53	130892.0
54	113573.0
55	96254.0
56	87724.5
57	79195.0
58	72001.5
59	64808.0
60	65160.0
61	65512.0
62	60977.5
63	56443.0
64	45555.5
65	34668.0
66	29745.5
67	24823.0
68	19657.0
69	14491.0
70	11465.0
71	8439.0
72	6780.0
73	5121.0
74	3814.0
75	2130.0
76	1753.0
77	1106.5
78	460.0
79	402.0
80	344.0
81	322.0
82	300.0
83	201.0
84	102.0
85	74.0
86	46.0
87	39.0
88	32.0
89	22.0
90	12.0
91	11.0
92	10.0
93	7.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2055644.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.171150955843345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.42959945499223	14.978406949263661
2	7.916342175927871	2.876980973937894
3	2.710810313295983	1.4777563024667488
4	1.265738686067949	0.9199971494076602
5	0.7595540642364925	0.6900985780182819
6	0.48943350831727434	0.5336142097488721
7	0.36404784008838015	0.46306177801762716
8	0.2536534474664733	0.36873400675066925
9	0.19156285776142096	0.3132825845323974
>10	1.735432383677518	6.708171880747421
>50	0.4686672638027402	6.208716717012722
>100	1.3831055105478167	59.45521144388132
>500	0.02908965825487337	3.4180361867142377
>1k	0.002424138187906114	0.5907021985430743
>5k	2.693486875451238E-4	0.4139662934192826
>10k+	2.693486875451238E-4	0.5832627475381403
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	11917	0.5797210022747129	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8458	0.411452566689563	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09471484362078259	0.0
2	0.0	0.0	0.0	0.3278777842856059	0.0
3	0.0	0.0	0.0	0.46423407944177103	0.0
4	0.0	0.0	0.0	0.7369953163096333	0.0
5	0.0	0.0	0.0	1.1882407654243634	0.0
6	0.0	0.0	0.0	1.6870625458493786	0.0
7	0.0	0.0	0.0	2.0151835629126444	0.0
8	0.0	0.0	0.0	2.9852445267760372	0.0
9	0.0	0.0	0.0	3.4442247782203532	0.0
10	0.0	0.0	0.0	4.24441197016604	0.0
11	0.0	0.0	0.0	4.6853443495079885	0.0
12	0.0	0.0	0.0	5.234661254575209	0.0
13	0.0	0.0	0.0	5.413680578932928	0.0
14	0.0	0.0	0.0	5.474245540570254	0.0
15	0.0	0.0	0.0	5.5889541185146845	0.0
16	0.0	0.0	0.0	5.829608628731434	0.0
17	0.0	0.0	0.0	6.108256098818667	0.0
18	0.0	0.0	0.0	6.67698297954315	0.0
19	0.0	0.0	0.0	6.82983045702466	0.0
20	0.0	0.0	0.0	7.03234606770433	0.0
21	0.0	0.0	0.0	7.202657658621824	0.0
22	0.0	0.0	0.0	7.346846049218639	0.0
23	0.0	0.0	0.0	7.515941476247833	0.0
24	0.0	0.0	0.0	7.632644562969074	0.0
25	0.0	0.0	0.0	7.723808208036022	0.0
26	0.0	0.0	0.0	7.81842575854574	0.0
27	0.0	0.0	0.0	7.908081360391196	0.0
28	0.0	0.0	0.0	7.999390945124739	0.0
29	0.0	0.0	0.0	8.099310970187444	0.0
30	0.0	0.0	0.0	8.254055663334702	0.0
31	0.0	0.0	0.0	8.389536320491292	0.0
32	0.0	0.0	0.0	8.505509708879552	0.0
33	0.0	0.0	0.0	8.627272037376121	0.0
34	0.0	0.0	0.0	8.744315649986087	0.0
35	0.0	0.0	0.0	8.90801130935123	0.0
36	4.8646555531989E-5	0.0	0.0	9.024130637406088	0.0
37	4.8646555531989E-5	0.0	0.0	9.139958086127754	0.0
38	4.8646555531989E-5	0.0	0.0	9.254374784738992	0.0
39	4.8646555531989E-5	0.0	0.0	9.36830501779491	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGA	35	1.2125565E-7	45.000004	23
TCTGCGA	35	1.2125565E-7	45.000004	1
AACGTTC	30	2.16634E-6	45.000004	14
TTGATCG	30	2.16634E-6	45.000004	39
ACGTTGC	60	0.0	45.000004	33
GCGCGTT	35	1.2125565E-7	45.000004	43
ACGTTAG	75	0.0	45.000004	1
GCAACTA	35	1.2125565E-7	45.000004	34
CGCATCG	35	1.2125565E-7	45.000004	21
GACCGGA	35	1.2125565E-7	45.000004	13
CACGCGA	30	2.16634E-6	45.000004	34
TCTAGTA	30	2.16634E-6	45.000004	30
CTGCGTA	30	2.16634E-6	45.000004	1
CATTACG	30	2.16634E-6	45.000004	33
TACGCTA	35	1.2125565E-7	45.000004	21
TCGGAAA	30	2.16634E-6	45.000004	43
TCTACCG	30	2.16634E-6	45.000004	15
CGTACTC	30	2.16634E-6	45.000004	43
CAATCGT	70	0.0	45.000004	13
TCGAATC	30	2.16634E-6	45.000004	12
>>END_MODULE