FastQCFastQC Report
Thu 26 May 2016
SRR1546358_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546358_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2130220
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102400.4807015237862756No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC37450.17580343814253926No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG28670.1345870379585207No Hit
GTACAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG25880.12148979917567199No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG24040.11285219367013735No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTCG302.1663782E-645.0000041
CACTACG207.034643E-445.00000425
TATTACG207.034643E-445.0000041
GACGATA207.034643E-445.00000445
TCGAATA207.034643E-445.00000426
CGTATCG253.8919316E-545.013
CGTTTTT73200.042.7868841
AAGTACG1250.041.3999981
CGCATCG1450.040.34482621
CGACGGT4750.040.26315728
TATAGCG1400.040.178571
TCACGAC4900.039.9489825
CGTAAGG3650.039.4520532
GTATGCG1600.039.3750041
ACGTCGT403.4600816E-739.37500442
TATCGCG750.039.01
TTATACG1050.038.571431
TAATGCG700.038.5714261
GTACAAG9350.038.5026741
TAACGCC2050.038.41463512