##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546358_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2130220
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11716911868258	31.0	31.0	33.0	30.0	34.0
2	31.44590699552159	31.0	31.0	34.0	30.0	34.0
3	31.52578513017435	31.0	31.0	34.0	30.0	34.0
4	35.383857535841365	37.0	35.0	37.0	33.0	37.0
5	35.28893353738111	37.0	35.0	37.0	33.0	37.0
6	35.33474946249683	37.0	35.0	37.0	33.0	37.0
7	35.77887119640225	37.0	35.0	37.0	35.0	37.0
8	35.68580756917126	37.0	35.0	37.0	35.0	37.0
9	37.44100374609195	39.0	37.0	39.0	35.0	39.0
10	36.89459586333806	39.0	37.0	39.0	32.0	39.0
11	36.61176639032588	39.0	35.0	39.0	32.0	39.0
12	35.99748335852635	37.0	35.0	39.0	32.0	39.0
13	35.7083596999371	37.0	35.0	39.0	31.0	39.0
14	36.52286712170574	38.0	35.0	41.0	31.0	41.0
15	36.789246650580694	38.0	35.0	41.0	31.0	41.0
16	36.89370065063702	38.0	35.0	41.0	31.0	41.0
17	36.838575827848764	38.0	35.0	41.0	31.0	41.0
18	36.76237947254274	38.0	35.0	40.0	31.0	41.0
19	36.69310963186901	38.0	35.0	40.0	31.0	41.0
20	36.526029236416896	38.0	35.0	40.0	31.0	41.0
21	36.36510407375764	38.0	35.0	40.0	31.0	41.0
22	36.301649594877524	38.0	35.0	40.0	30.0	41.0
23	36.26080921219405	38.0	35.0	40.0	30.0	41.0
24	36.25050323440772	38.0	35.0	40.0	30.0	41.0
25	36.18108927716386	38.0	35.0	40.0	30.0	41.0
26	36.07118372750232	38.0	35.0	40.0	30.0	41.0
27	35.96809015031312	38.0	34.0	40.0	30.0	41.0
28	35.91153120334989	38.0	34.0	40.0	30.0	41.0
29	35.79561735407611	37.0	34.0	40.0	30.0	41.0
30	35.636776013745056	37.0	34.0	40.0	29.0	41.0
31	35.39832740280347	37.0	34.0	40.0	28.0	41.0
32	35.22764972631935	37.0	34.0	40.0	27.0	41.0
33	35.046364225291285	37.0	34.0	40.0	26.0	41.0
34	34.811622743190846	37.0	34.0	40.0	25.0	41.0
35	34.57833604040897	37.0	33.0	40.0	24.0	41.0
36	34.405139375275795	37.0	33.0	40.0	23.0	41.0
37	34.2258663424435	36.0	33.0	40.0	23.0	41.0
38	34.12136633774915	36.0	33.0	40.0	23.0	41.0
39	34.04907427401864	36.0	33.0	40.0	23.0	41.0
40	33.83675911408211	36.0	33.0	40.0	22.0	41.0
41	33.7273281632883	36.0	33.0	40.0	22.0	41.0
42	33.63112870971074	36.0	32.0	40.0	22.0	41.0
43	33.5894245664767	35.0	32.0	40.0	22.0	41.0
44	33.4259053055553	35.0	32.0	40.0	22.0	41.0
45	33.36375210072199	35.0	32.0	40.0	22.0	41.0
46	33.302730703870964	35.0	32.0	39.0	22.0	41.0
47	33.21908112777084	35.0	32.0	39.0	21.0	41.0
48	33.120860286730945	35.0	32.0	39.0	20.0	41.0
49	33.129917567199634	35.0	32.0	39.0	21.0	41.0
50	32.96922571377604	35.0	32.0	39.0	20.0	41.0
51	32.75757245730488	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	4.0
13	14.0
14	28.0
15	110.0
16	251.0
17	672.0
18	1605.0
19	2899.0
20	4890.0
21	7877.0
22	11564.0
23	16602.0
24	22936.0
25	31460.0
26	40715.0
27	46927.0
28	51244.0
29	56717.0
30	65665.0
31	78840.0
32	96350.0
33	122474.0
34	194353.0
35	243512.0
36	166517.0
37	210539.0
38	287184.0
39	368227.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.23245486381688	22.719202711457033	28.241965618574604	12.806376806151476
2	33.93269239796828	24.023903634366402	29.37480635802875	12.668597609636564
3	30.740533841575047	24.627174658016543	30.89572907962558	13.73656242078283
4	26.947967815530788	27.02354686370422	30.642797457539594	15.385687863225394
5	22.40853057430688	32.54316455577358	30.074264629944324	14.974040239975213
6	20.44685525438687	41.40882162405761	27.89913717831961	10.24518594323591
7	86.94519814854804	4.130981776530124	7.07128841152557	1.8525316633962687
8	87.98748486071861	3.0402024204072817	6.842626583169814	2.1296861357042935
9	83.7723333740177	4.457802480494973	8.62530630639089	3.1445578390964313
10	46.20771563500484	24.711391311695504	15.321328313507527	13.759564739792134
11	38.56320004506576	23.331346058153617	21.827557717043312	16.277896179737304
12	34.92873975457934	21.58551698885561	27.478382514482075	16.007360742082977
13	26.088150519664637	29.31443700650637	28.157795908403827	16.439616565425165
14	20.1208795335693	32.90800950136605	30.131019331336667	16.840091633727972
15	17.986592934063147	23.63154979297913	41.06026607580438	17.321591197153346
16	21.82427167147055	19.32565650496193	38.50203265390429	20.348039169663227
17	21.92294692566965	19.57398766324605	29.339504839875698	29.163560571208606
18	24.63229150040841	22.48913257785581	31.950502764972633	20.92807315676315
19	29.150181671376664	25.318136154951134	25.559613561040646	19.97206861263156
20	29.58858709429073	24.669329928364206	24.09342696998432	21.648656007360742
21	24.9550281191614	25.98041516838636	28.603712292627055	20.460844419825182
22	23.78486729070237	22.61066932053966	27.24939208156904	26.355071307188926
23	21.52214325280957	27.67052229347204	26.76249401470271	24.044840439015687
24	22.579874379172104	25.413196759020195	31.863845048868196	20.14308381293951
25	23.463961468768485	23.71867694416539	29.7434067842758	23.073954802790322
26	20.31100074170743	27.318774586662407	27.200617776567675	25.16960689506248
27	20.203030672888246	27.11940550741238	29.436161523222953	23.241402296476423
28	19.500004694350817	26.057402521805262	32.79543896874501	21.64715381509891
29	20.043892180150408	25.789965355690963	32.188083859883015	21.978058604275617
30	23.802236388729803	25.308888283839227	30.167212776145185	20.72166255128578
31	29.11671095004272	23.76576128287219	25.892912469134643	21.224615297950447
32	28.830026945573696	24.81898583244923	25.402916130728283	20.948071091248792
33	27.600435635755932	23.812563960529896	26.354320211058013	22.232680192656158
34	22.534761667808958	24.957844729652336	29.271014261437784	23.236379341100918
35	21.787702678596577	25.444508078977755	29.946343570147683	22.82144567227798
36	29.760259503713232	24.74256180112852	25.891269446348264	19.605909248809983
37	23.113575123696144	29.3550900845922	26.573546394269137	20.957788397442517
38	23.850353484616612	29.771150397611514	23.871055571724987	22.507440546046887
39	22.250941217339054	27.169259513101935	25.93849461557961	24.6413046539794
40	24.73711635417938	24.106148660701713	27.071992564148307	24.084742420970603
41	21.33282008431054	22.775300203734826	27.647566917970913	28.244312793983724
42	22.679019068453023	25.04689656467407	25.674202664513523	26.59988170235938
43	22.392006459426725	25.405075532104664	26.34633981466703	25.856578193801578
44	22.492230849395835	25.87422895287811	28.072311780003943	23.561228417722113
45	21.419853348480437	28.577330041028624	25.938729333120524	24.06408727737041
46	23.34618020673921	27.561754185013754	27.30642844401048	21.785637164236558
47	22.40294429683319	26.47627944531551	27.824684774342558	23.296091483508746
48	22.35816019002732	25.748561181474216	29.030522669020105	22.862755959478363
49	22.789383256189502	23.7234182384918	30.156227995230537	23.330970510088157
50	21.81901399855414	26.964069438837303	28.770127029133143	22.446789533475417
51	21.772399094929163	28.521701983832653	26.520406343006826	23.185492578231358
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	799.0
1	1752.5
2	2706.0
3	10832.0
4	18958.0
5	13754.5
6	8551.0
7	8624.0
8	8697.0
9	8806.0
10	8915.0
11	8908.5
12	8902.0
13	8670.5
14	8439.0
15	8101.5
16	7764.0
17	7407.5
18	7051.0
19	6771.5
20	6492.0
21	6831.5
22	7171.0
23	7607.5
24	8044.0
25	9106.0
26	11581.5
27	12995.0
28	15606.5
29	18218.0
30	22472.0
31	26726.0
32	31119.0
33	35512.0
34	40366.5
35	45221.0
36	50986.5
37	56752.0
38	61609.5
39	66467.0
40	86759.5
41	107052.0
42	124227.5
43	141403.0
44	148286.5
45	155170.0
46	162704.5
47	170239.0
48	169688.0
49	169137.0
50	168194.5
51	167252.0
52	156629.0
53	146006.0
54	136829.0
55	127652.0
56	126635.0
57	125618.0
58	119456.5
59	113295.0
60	104089.0
61	94883.0
62	85429.0
63	75975.0
64	66259.0
65	56543.0
66	47420.5
67	38298.0
68	32177.5
69	26057.0
70	22021.0
71	17985.0
72	14230.5
73	10476.0
74	8325.0
75	4575.5
76	2977.0
77	2291.5
78	1606.0
79	1293.5
80	981.0
81	748.0
82	515.0
83	343.5
84	172.0
85	127.5
86	83.0
87	74.5
88	66.0
89	53.0
90	40.0
91	27.0
92	14.0
93	8.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2130220.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.934563971094647
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.05528363760742	16.75922456535619
2	6.854634409174533	2.8699756507466163
3	2.3729330115642844	1.4902895378914438
4	1.294831712420746	1.0842694926189653
5	0.9091389116809799	0.9516213352598422
6	0.6606216314135602	0.8297895482109652
7	0.5138536106198852	0.7530110898309963
8	0.448444035635007	0.7510384281165522
9	0.35789926231322966	0.6743218501893501
>10	4.17165016820362	20.039308046922496
>50	1.326021378646937	19.97917990097251
>100	1.0234269311720157	30.940118322497685
>500	0.006982005719654407	0.9492725436545927
>1k	0.004054067837218688	1.4457628771708526
>5k	0.0	0.0
>10k+	2.2522599095659381E-4	0.4828168105609213
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10240	0.4807015237862756	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3745	0.17580343814253926	No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	2867	0.1345870379585207	No Hit
GTACAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	2588	0.12148979917567199	No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	2404	0.11285219367013735	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.6943508182253474E-5	0.0	0.0	0.07515655659978782	0.0
2	4.6943508182253474E-5	0.0	0.0	0.22992930307667753	0.0
3	4.6943508182253474E-5	0.0	0.0	0.3189811380984124	0.0
4	4.6943508182253474E-5	0.0	0.0	0.452441531860559	0.0
5	4.6943508182253474E-5	0.0	0.0	0.7329759367577058	0.0
6	4.6943508182253474E-5	0.0	0.0	1.017078048276704	0.0
7	4.6943508182253474E-5	0.0	0.0	1.2057440076611805	0.0
8	4.6943508182253474E-5	0.0	0.0	1.7557341495244623	0.0
9	4.6943508182253474E-5	0.0	0.0	2.0150970322314126	0.0
10	4.6943508182253474E-5	0.0	0.0	2.4450995671808546	0.0
11	4.6943508182253474E-5	0.0	0.0	2.760747716198327	0.0
12	4.6943508182253474E-5	0.0	0.0	3.0597309198110993	0.0
13	4.6943508182253474E-5	0.0	0.0	3.1759161025621765	0.0
14	4.6943508182253474E-5	0.0	0.0	3.2218268535644206	0.0
15	4.6943508182253474E-5	0.0	0.0	3.2869374994132063	0.0
16	4.6943508182253474E-5	0.0	0.0	3.4412408108082735	0.0
17	4.6943508182253474E-5	0.0	0.0	3.6166687008853544	0.0
18	4.6943508182253474E-5	0.0	0.0	3.8990808461097917	0.0
19	9.388701636450695E-5	0.0	0.0	4.007003971420792	0.0
20	1.4083052454676043E-4	0.0	0.0	4.144689280919342	0.0
21	1.4083052454676043E-4	0.0	0.0	4.296410699364385	0.0
22	1.4083052454676043E-4	0.0	0.0	4.449305705513985	0.0
23	1.4083052454676043E-4	0.0	0.0	4.625203030672888	0.0
24	1.4083052454676043E-4	0.0	0.0	4.756926514632291	0.0
25	1.4083052454676043E-4	0.0	0.0	4.863582165222371	0.0
26	1.4083052454676043E-4	0.0	0.0	4.972444160697017	0.0
27	1.4083052454676043E-4	0.0	0.0	5.104449305705514	0.0
28	1.4083052454676043E-4	0.0	0.0	5.224389969111171	0.0
29	1.4083052454676043E-4	0.0	0.0	5.3719334153279945	0.0
30	1.4083052454676043E-4	0.0	0.0	5.5528536958623995	0.0
31	1.4083052454676043E-4	0.0	0.0	5.695702791260996	0.0
32	1.4083052454676043E-4	0.0	0.0	5.83700275088958	0.0
33	1.877740327290139E-4	0.0	0.0	5.98698725953188	0.0
34	1.877740327290139E-4	0.0	0.0	6.151289538169767	0.0
35	1.877740327290139E-4	0.0	0.0	6.3456356620442955	0.0
36	1.877740327290139E-4	0.0	0.0	6.492334125113838	0.0
37	1.877740327290139E-4	0.0	0.0	6.641426707100675	0.0
38	1.877740327290139E-4	0.0	0.0	6.839481368121603	0.0
39	1.877740327290139E-4	0.0	0.0	7.1900085437184895	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGTCG	30	2.1663782E-6	45.000004	1
CACTACG	20	7.034643E-4	45.000004	25
TATTACG	20	7.034643E-4	45.000004	1
GACGATA	20	7.034643E-4	45.000004	45
TCGAATA	20	7.034643E-4	45.000004	26
CGTATCG	25	3.8919316E-5	45.0	13
CGTTTTT	7320	0.0	42.786884	1
AAGTACG	125	0.0	41.399998	1
CGCATCG	145	0.0	40.344826	21
CGACGGT	475	0.0	40.263157	28
TATAGCG	140	0.0	40.17857	1
TCACGAC	490	0.0	39.94898	25
CGTAAGG	365	0.0	39.452053	2
GTATGCG	160	0.0	39.375004	1
ACGTCGT	40	3.4600816E-7	39.375004	42
TATCGCG	75	0.0	39.0	1
TTATACG	105	0.0	38.57143	1
TAATGCG	70	0.0	38.571426	1
GTACAAG	935	0.0	38.502674	1
TAACGCC	205	0.0	38.414635	12
>>END_MODULE