##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546357_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1852560
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.131438657857235	31.0	31.0	33.0	30.0	34.0
2	31.451699270199075	31.0	31.0	34.0	30.0	34.0
3	31.53449173036231	31.0	31.0	34.0	30.0	34.0
4	35.3797285917865	37.0	35.0	37.0	33.0	37.0
5	35.28537213369607	37.0	35.0	37.0	33.0	37.0
6	35.33338083516863	37.0	35.0	37.0	33.0	37.0
7	35.78020361013948	37.0	35.0	37.0	35.0	37.0
8	35.68975687697024	37.0	35.0	37.0	35.0	37.0
9	37.45555339638123	39.0	37.0	39.0	35.0	39.0
10	36.93137280304012	39.0	37.0	39.0	32.0	39.0
11	36.59063728030401	39.0	35.0	39.0	32.0	39.0
12	35.69290981128816	37.0	35.0	39.0	31.0	39.0
13	35.21543269853608	37.0	35.0	39.0	30.0	39.0
14	35.928932396251675	38.0	35.0	40.0	27.0	41.0
15	36.33927376171352	38.0	35.0	40.0	30.0	41.0
16	36.5265308546012	38.0	35.0	40.0	31.0	41.0
17	36.53619154035497	38.0	35.0	40.0	31.0	41.0
18	36.47379302154856	38.0	35.0	40.0	31.0	41.0
19	36.34986181284277	37.0	35.0	40.0	31.0	41.0
20	36.15657738480805	37.0	35.0	40.0	31.0	41.0
21	35.967697132616486	37.0	34.0	40.0	30.0	41.0
22	35.89760277669819	37.0	34.0	40.0	30.0	41.0
23	35.89714179297837	37.0	34.0	40.0	30.0	41.0
24	35.857772487800666	37.0	34.0	40.0	30.0	41.0
25	35.809479315109904	36.0	34.0	40.0	30.0	41.0
26	35.68100034546789	36.0	34.0	40.0	30.0	41.0
27	35.55641598652675	36.0	34.0	40.0	30.0	41.0
28	35.502499784082566	36.0	34.0	40.0	29.0	41.0
29	35.42572872133696	36.0	34.0	40.0	29.0	41.0
30	35.26424515265362	36.0	34.0	40.0	29.0	41.0
31	35.02457356306948	36.0	34.0	40.0	27.0	41.0
32	34.839925292568125	36.0	34.0	40.0	27.0	41.0
33	34.634789696420086	36.0	33.0	40.0	25.0	41.0
34	34.421567452606126	36.0	33.0	40.0	24.0	41.0
35	34.25114112363432	35.0	33.0	40.0	24.0	41.0
36	34.00976216694736	35.0	33.0	40.0	23.0	41.0
37	33.795585568078764	35.0	33.0	40.0	23.0	41.0
38	33.69518180247873	35.0	33.0	40.0	23.0	41.0
39	33.634486332426484	35.0	33.0	40.0	23.0	41.0
40	33.3784697931511	35.0	32.0	40.0	21.0	41.0
41	33.304158569762926	35.0	32.0	40.0	21.0	41.0
42	33.20518039901542	35.0	32.0	39.0	21.0	41.0
43	33.168826380791984	35.0	32.0	39.0	21.0	41.0
44	33.00268817204301	35.0	32.0	39.0	21.0	41.0
45	33.003028781793844	35.0	32.0	39.0	21.0	41.0
46	32.98209072850542	35.0	32.0	39.0	21.0	41.0
47	32.887291099883406	35.0	32.0	39.0	20.0	41.0
48	32.786071706179555	35.0	32.0	39.0	20.0	41.0
49	32.788827352420434	35.0	31.0	39.0	20.0	40.0
50	32.60902804767457	35.0	31.0	38.0	20.0	40.0
51	32.40560413697802	35.0	31.0	38.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	0.0
11	3.0
12	1.0
13	5.0
14	22.0
15	103.0
16	275.0
17	701.0
18	1479.0
19	2846.0
20	4890.0
21	7771.0
22	11352.0
23	15660.0
24	21677.0
25	29925.0
26	38323.0
27	43352.0
28	47031.0
29	52099.0
30	60402.0
31	71560.0
32	88498.0
33	112996.0
34	191632.0
35	252090.0
36	131409.0
37	162704.0
38	218498.0
39	285203.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.88218465258885	22.541132271019563	27.873807056181715	13.702876020209873
2	31.20665457529041	24.969015848339595	30.848555512372066	12.975774063997928
3	30.53552921362871	24.409411840912036	31.634225072332338	13.420833873126917
4	26.1797728548603	26.82957636999611	31.34333031048927	15.647320464654316
5	22.591441032949	32.40753335924342	30.28042276633415	14.72060284147342
6	20.79997408990802	40.502385887636564	28.043572138014422	10.654067884440991
7	87.16192727900851	4.247203869240403	7.022336658461804	1.5685321932892862
8	88.46067063954743	2.9845187200414562	6.498035151358121	2.056775489052986
9	84.56136373450792	4.346957723366584	8.226184307120956	2.865494235004534
10	53.06295072764174	20.850930604136977	13.931478602582374	12.1546400656389
11	45.74567085546487	20.45509996977156	19.143887377466857	14.655341797296714
12	40.91414043269854	20.477987217687957	24.63455974435376	13.97331260525975
13	24.94790991924688	34.279969339724495	26.071436282765468	14.700684458263162
14	18.44177786414475	37.7384268255819	29.172280519929178	14.647514790344172
15	15.876624778684631	21.94487627931079	46.575279613075956	15.603219328928617
16	18.660232327158095	16.665749017575678	43.63048970073844	21.043528954527787
17	19.294705704538583	17.488556376041803	28.314224640497475	34.90251327892214
18	24.276028846569073	20.883264239754716	34.009370816599734	20.831336097076477
19	29.42458004059248	23.870805803860602	25.776277151617222	20.9283370039297
20	30.037137798505853	23.50644513538023	24.053849807833487	22.402567258280435
21	23.793615321501058	25.37510256078076	29.61852787494062	21.212754242777564
22	23.03731053245239	22.5057218119791	27.534006995724837	26.922960659843675
23	20.44997193073369	28.665468324912553	25.73514488059766	25.149414863756096
24	20.971790387355878	25.473506930949608	34.64773070777735	18.906971973917173
25	20.718303320810122	22.448125836680052	33.03655482143628	23.797016021073542
26	18.35508701472557	29.61501921665155	27.361273049185993	24.668620719436888
27	19.674180593341106	28.676048279138055	29.92075830202531	21.72901282549553
28	17.38097551496308	25.560953491384897	36.46661916483137	20.59145182882066
29	17.81000345467893	25.0661247139094	35.43329230902103	21.69057952239064
30	22.075290408947616	26.74018655266226	31.413395517554086	19.771127520836032
31	29.75083128211772	23.54531027335147	24.783974608109858	21.919883836420954
32	30.114598177656866	24.759575938161245	25.58000820486246	19.545817679319427
33	28.442857451310623	25.002267133048324	25.797059204560174	20.757816211080883
34	20.631342574599472	25.86734032905817	29.451137884872825	24.050179211469533
35	21.705801701429372	25.46562594463877	29.786241741158182	23.042330612773675
36	31.52686012868679	22.819179945588807	26.21588504555858	19.438074880165825
37	22.123817852053374	29.55143153258194	27.51095780973356	20.813792805631127
38	23.543313037094617	30.265632422161765	22.724338213067323	23.466716327676295
39	21.576629097033294	26.64199809992659	25.988416029710237	25.792956773329877
40	24.33729541823207	23.78157792460163	25.476043960789397	26.40508269637691
41	19.82958716586777	22.335686833354927	27.27447424105022	30.56025175972708
42	23.75172733946539	24.358131450533314	24.964859437751006	26.925281772250294
43	23.10796951245844	24.61086280606296	26.321954484605087	25.959213196873517
44	21.862287861121906	27.662369909746516	27.55052467936261	22.92481754976897
45	19.473107483698236	31.119963725871223	24.19317053158872	25.213758258841818
46	23.648464827050137	27.77691410804508	27.110053115688558	21.46456794921622
47	23.44938895366412	25.67258280433562	27.76422895884614	23.113799283154123
48	23.309636395042535	25.579954225504164	27.422053806624348	23.68835557282895
49	22.64434080407652	22.401001856889927	30.37947488880252	24.575182450231033
50	21.140799758172474	27.69178866001641	27.797966057779504	23.369445524031608
51	20.665673446474067	30.84040462926977	24.415889363907244	24.078032560348923
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	536.0
1	1157.0
2	1778.0
3	8840.5
4	15903.0
5	11403.5
6	6904.0
7	7352.5
8	7801.0
9	8033.5
10	8266.0
11	8254.0
12	8242.0
13	7866.0
14	7490.0
15	7092.5
16	6695.0
17	6141.5
18	5588.0
19	5635.0
20	5682.0
21	5422.0
22	5162.0
23	5262.5
24	5363.0
25	6253.0
26	8273.5
27	9404.0
28	10504.5
29	11605.0
30	15392.0
31	19179.0
32	22670.5
33	26162.0
34	28385.5
35	30609.0
36	33169.0
37	35729.0
38	44567.0
39	53405.0
40	79493.0
41	105581.0
42	126040.5
43	146500.0
44	152997.5
45	159495.0
46	159880.0
47	160265.0
48	158948.0
49	157631.0
50	152187.0
51	146743.0
52	141521.0
53	136299.0
54	121787.0
55	107275.0
56	106364.0
57	105453.0
58	97387.5
59	89322.0
60	81025.0
61	72728.0
62	66741.5
63	60755.0
64	52297.5
65	43840.0
66	37621.5
67	31403.0
68	25462.5
69	19522.0
70	16286.0
71	13050.0
72	10472.5
73	7895.0
74	6423.0
75	3683.5
76	2416.0
77	1864.0
78	1312.0
79	974.5
80	637.0
81	553.5
82	470.0
83	360.5
84	251.0
85	164.0
86	77.0
87	52.5
88	28.0
89	19.0
90	10.0
91	7.0
92	4.0
93	2.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1852560.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.43728142706953
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.23048488885334	15.788997952419608
2	7.176723767213092	2.78991999175319
3	2.4838836928306964	1.448398391089769
4	1.2177630466764604	0.9468001219894387
5	0.7845335489671976	0.7624599690126528
6	0.5776873149783546	0.6737202550849462
7	0.47248688693096547	0.6428702414314009
8	0.3734515205089483	0.5807105842799565
9	0.29508001546115414	0.5161997973620233
>10	3.031873122750993	13.159518605265383
>50	0.9577578931218987	13.668023799388385
>100	1.3834880810384995	45.71859074755024
>500	0.011438397120814285	1.4683087314880823
>1k	0.002789852956296167	0.9674667089588536
>5k	5.579705912592334E-4	0.8680141029260898
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9761	0.5268925163017661	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6246	0.33715507190050525	No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	3217	0.17365159562983115	No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	2606	0.14067020771257072	No Hit
GTACAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1946	0.10504383123893424	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.050632638079198516	0.0
2	0.0	0.0	0.0	0.19437966921449237	0.0
3	0.0	0.0	0.0	0.2761044176706827	0.0
4	0.0	0.0	0.0	0.4117005657036749	0.0
5	0.0	0.0	0.0	0.6807336874379237	0.0
6	0.0	0.0	0.0	0.9608325776223172	0.0
7	0.0	0.0	0.0	1.1624454808481237	0.0
8	0.0	0.0	0.0	1.750874465604353	0.0
9	0.0	0.0	0.0	2.028814181457011	0.0
10	0.0	0.0	0.0	2.4774366282333635	0.0
11	0.0	0.0	0.0	2.7856587640886126	0.0
12	0.0	0.0	0.0	3.0985771041153862	0.0
13	0.0	0.0	0.0	3.211501921665155	0.0
14	0.0	0.0	0.0	3.2529040894761843	0.0
15	0.0	0.0	0.0	3.318435030444358	0.0
16	0.0	0.0	0.0	3.4763246534525196	0.0
17	0.0	0.0	0.0	3.650677980740165	0.0
18	0.0	0.0	0.0	3.9590080753120005	0.0
19	0.0	0.0	0.0	4.062432525802134	0.0
20	0.0	0.0	0.0	4.190471563674051	0.0
21	0.0	0.0	0.0	4.333948698017878	0.0
22	0.0	0.0	0.0	4.483417541132271	0.0
23	0.0	0.0	0.0	4.642386751306301	0.0
24	0.0	0.0	0.0	4.7619510299261565	0.0
25	0.0	0.0	0.0	4.850369218810727	0.0
26	0.0	0.0	0.0	4.955143153258194	0.0
27	0.0	0.0	0.0	5.06882368182407	0.0
28	0.0	0.0	0.0	5.186390724187071	0.0
29	0.0	0.0	0.0	5.319719739171741	0.0
30	0.0	0.0	0.0	5.492507665068878	0.0
31	0.0	0.0	0.0	5.631936347540701	0.0
32	0.0	0.0	0.0	5.757060500064775	0.0
33	0.0	0.0	0.0	5.891415122857019	0.0
34	0.0	0.0	0.0	6.033002979660578	0.0
35	0.0	0.0	0.0	6.2170186120827395	0.0
36	0.0	0.0	0.0	6.363788487282463	0.0
37	0.0	0.0	0.0	6.504188798203567	0.0
38	0.0	0.0	0.0	6.710767802392366	0.0
39	0.0	0.0	0.0	7.113561773977631	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	50	2.1827873E-11	45.0	1
CCTTACG	25	3.891733E-5	45.0	1
TACCCGA	20	7.0344063E-4	45.0	43
AAATCCG	20	7.0344063E-4	45.0	32
CTCGTTA	25	3.891733E-5	45.0	35
CGTATTA	35	1.2124656E-7	45.0	44
CGTGATA	20	7.0344063E-4	45.0	44
CTATACG	20	7.0344063E-4	45.0	1
GCTATCG	25	3.891733E-5	45.0	27
CCTCGTA	25	3.891733E-5	45.0	14
CGTTACA	20	7.0344063E-4	45.0	40
CTAACGG	55	1.8189894E-12	45.0	2
CTACGCA	25	3.891733E-5	45.0	1
ACTACGT	20	7.0344063E-4	45.0	13
CATACCG	20	7.0344063E-4	45.0	39
CGTAAAT	80	0.0	45.0	16
ATCGGAT	25	3.891733E-5	45.0	34
CGACTAA	20	7.0344063E-4	45.0	35
TATCGGC	25	3.891733E-5	45.0	29
AATGTCG	20	7.0344063E-4	45.0	1
>>END_MODULE