FastQCFastQC Report
Thu 26 May 2016
SRR1546356_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546356_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1443113
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72510.5024554556711776No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC44000.30489642876198886No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACACT253.8913022E-545.00000411
CGTAGTG253.8913022E-545.00000413
TTGCGAA253.8913022E-545.0000041
ACCGTTC502.1827873E-1145.00000415
GCTACGT253.8913022E-545.00000421
CAATCGA253.8913022E-545.00000445
TTTTACG551.8189894E-1245.0000041
TACCGTT502.1827873E-1145.00000414
TATCACG207.0338865E-445.01
CGTGATA207.0338865E-445.02
CGTTATG207.0338865E-445.03
ACCCGGT207.0338865E-445.015
TATCGTT207.0338865E-445.09
CCCATCG207.0338865E-445.018
AATCGCG406.8175723E-945.01
TACGTAG700.045.01
GCACGAC302.1658907E-644.99999615
CTACCGT302.1658907E-644.99999635
GTTCACG302.1658907E-644.9999961
CGTCGAA302.1658907E-644.99999638
Produced by FastQC (version 0.11.5)