##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546356_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1443113
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07671055558366	31.0	31.0	33.0	30.0	34.0
2	31.39091117604789	31.0	31.0	34.0	30.0	34.0
3	31.452518271264967	31.0	31.0	34.0	30.0	34.0
4	35.306795101977464	37.0	35.0	37.0	33.0	37.0
5	35.21219266959691	37.0	35.0	37.0	32.0	37.0
6	35.2543051029268	37.0	35.0	37.0	32.0	37.0
7	35.74956084520062	37.0	35.0	37.0	35.0	37.0
8	35.667139025149105	37.0	35.0	37.0	35.0	37.0
9	37.40382700453811	39.0	37.0	39.0	35.0	39.0
10	36.891488746896464	39.0	37.0	39.0	32.0	39.0
11	36.5263038999718	39.0	35.0	39.0	32.0	39.0
12	35.621176581459665	37.0	35.0	39.0	31.0	39.0
13	35.162226381440675	37.0	35.0	39.0	30.0	39.0
14	36.024045241086455	38.0	35.0	40.0	30.0	41.0
15	36.36997726442767	38.0	35.0	40.0	31.0	41.0
16	36.51357793880313	38.0	35.0	40.0	31.0	41.0
17	36.44747500715467	38.0	35.0	40.0	31.0	41.0
18	36.35248175298816	38.0	35.0	40.0	31.0	41.0
19	36.223351878889595	37.0	35.0	40.0	31.0	41.0
20	36.053715821283575	37.0	34.0	40.0	30.0	41.0
21	35.88265922349809	36.0	34.0	40.0	30.0	41.0
22	35.76661356387199	36.0	34.0	40.0	30.0	41.0
23	35.76283215520891	36.0	34.0	40.0	30.0	41.0
24	35.736166190727964	36.0	34.0	40.0	30.0	41.0
25	35.65233976826485	36.0	34.0	40.0	30.0	41.0
26	35.50992403228299	36.0	34.0	40.0	30.0	41.0
27	35.38639940184864	36.0	34.0	40.0	29.0	41.0
28	35.35385378691759	36.0	34.0	40.0	29.0	41.0
29	35.34775377950306	36.0	34.0	40.0	29.0	41.0
30	35.190274774047495	36.0	34.0	40.0	29.0	41.0
31	34.954117938096324	36.0	34.0	40.0	27.0	41.0
32	34.71102332249796	35.0	34.0	40.0	26.0	41.0
33	34.4823038805693	35.0	33.0	40.0	25.0	41.0
34	34.25771162757179	35.0	33.0	40.0	24.0	41.0
35	34.06070487896651	35.0	33.0	40.0	23.0	41.0
36	33.82964951462567	35.0	33.0	40.0	23.0	41.0
37	33.65295995531881	35.0	33.0	40.0	22.0	41.0
38	33.62509519351568	35.0	33.0	40.0	23.0	41.0
39	33.57949446786218	35.0	33.0	40.0	22.0	41.0
40	33.3445080184296	35.0	32.0	40.0	21.0	41.0
41	33.28504282062458	35.0	32.0	40.0	21.0	41.0
42	33.183180388507346	35.0	32.0	39.0	20.0	41.0
43	33.13465889365559	35.0	32.0	39.0	20.0	41.0
44	32.960765373189766	35.0	32.0	39.0	19.0	41.0
45	32.935391060852474	35.0	32.0	39.0	20.0	41.0
46	32.85450827481978	35.0	32.0	39.0	20.0	41.0
47	32.78815657540331	35.0	31.0	39.0	20.0	41.0
48	32.730946918224696	35.0	31.0	39.0	19.0	41.0
49	32.72931087170582	35.0	31.0	39.0	19.0	41.0
50	32.5571282359732	35.0	31.0	39.0	18.0	40.0
51	32.31715257225179	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	8.0
13	13.0
14	28.0
15	58.0
16	187.0
17	499.0
18	1152.0
19	2281.0
20	3869.0
21	6062.0
22	8993.0
23	12927.0
24	17822.0
25	24752.0
26	31443.0
27	35627.0
28	37867.0
29	41482.0
30	47568.0
31	56395.0
32	68890.0
33	88573.0
34	150803.0
35	201916.0
36	97382.0
37	118137.0
38	163394.0
39	224932.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.04807939503005	24.168724140105454	29.392639384441825	14.390557080422669
2	31.08571539442857	26.165310685996175	30.498166117275638	12.250807802299612
3	28.14187108008867	25.349712739057857	33.20827960111232	13.300136579741157
4	24.99617147097975	27.511289829694558	32.29760940411458	15.194929295211116
5	21.897592219043137	32.26185336837795	31.422002296424466	14.418552116154451
6	20.150674271522742	40.056045507177885	29.038405169934716	10.754875051364653
7	85.19339788360301	4.578990002861868	8.422763844549943	1.8048482689851728
8	86.63867625057775	3.3375071806573704	7.650821522638907	2.372995046125979
9	82.14450289062603	5.0966902799711455	9.765486140032001	2.9933206893708255
10	51.51245952326672	24.044548140027842	13.64550107995701	10.797491256748431
11	44.868558456614274	19.99524638749703	22.152804388845503	12.98339076704319
12	41.74129122251688	19.94902686068243	24.716359702947724	13.593322213852968
13	22.494842746202135	37.946231514787826	25.10475617640476	14.454169562605285
14	16.100125215419723	39.919604355306895	28.069319588971897	15.910950840301489
15	14.512723535856168	22.4432875318842	47.93373769067287	15.110251241586765
16	16.4183262156186	17.52752556452613	46.06160432343136	19.99254389642391
17	17.271689742937664	18.043077707705496	29.466853946988213	35.21837860236863
18	21.837236585076845	22.86695497857756	34.50346577156467	20.79234266478093
19	28.86530715196939	24.802631533358788	25.29372266759429	21.038338647077534
20	30.874366733582193	22.823715121407677	25.602638185644505	20.699279959365622
21	22.501009969420274	27.860396240627033	28.0091025442914	21.62949124566129
22	22.021075272691743	24.18493908654416	26.00634877518254	27.78763686558156
23	19.246517770957645	30.0171227062607	25.74531585537654	24.991043667405116
24	21.81374570113359	21.765655218960678	37.74756377359223	18.673035306313505
25	18.42399035972928	24.095895470417076	34.12317677132698	23.356937398526657
26	17.394202671585663	31.326999341007944	28.407616035611905	22.87118195179449
27	18.610739422345997	30.312595063588226	30.01767706340391	21.058988450661868
28	15.21072847379242	26.9353820525489	38.32811429181221	19.525775181846466
29	16.517417554966244	22.86397530893284	36.42854024598212	24.190066890118793
30	19.54753369971721	28.098076865775585	31.721008680539914	20.63338075396729
31	28.1314075890107	24.85986890839456	25.81481838220569	21.193905120389047
32	29.208939286112727	25.2337828014854	25.98202635552448	19.57525155687739
33	26.551559025523296	26.823817677479173	25.479917373067806	21.144705923929727
34	19.187340145920658	26.5681204451765	29.824622188283247	24.419917220619592
35	19.652584378354295	25.84551590901059	30.549582742307773	23.95231697032734
36	27.77959868700511	25.375836819431324	27.931146071028394	18.913418422535173
37	20.18081744118444	31.127361474811742	28.873206741260038	19.818614342743775
38	21.08379593282023	31.29103542134261	24.95320879238147	22.67195985345569
39	21.300480281169943	30.560531295886044	26.531186400510563	21.607802022433447
40	23.761895291636897	25.73679261429978	25.660776391037988	24.840535703025335
41	17.516923484162366	24.38450765809746	27.30285154385	30.79571731389018
42	22.202280763876423	25.789941605404426	23.87844888099546	28.129328749723687
43	21.993634594103163	26.435767677236637	25.663686766039806	25.906910962620387
44	20.2824726823194	29.697743697132516	26.43736145402335	23.582422166524726
45	18.203910573877444	33.91570861048303	23.07712563049463	24.80325518514489
46	22.85018567499565	30.519578161931882	25.896239587613724	20.73399657545875
47	21.950602620861982	26.15463931098951	27.46451594573675	24.43024212241176
48	23.88316091671269	22.15363592456031	29.826423848998658	24.136779309728343
49	21.02538054885515	21.947553656574364	31.719276314467404	25.307789480103082
50	19.14617912803779	29.332699518332937	28.7594249376175	22.761696416011777
51	18.513934806214067	30.478070670834505	25.238356247916833	25.769638275034595
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	944.0
1	1230.0
2	1516.0
3	7679.5
4	13843.0
5	10415.5
6	6988.0
7	7236.5
8	7485.0
9	7842.5
10	8200.0
11	8217.0
12	8234.0
13	7870.5
14	7507.0
15	7120.0
16	6733.0
17	6406.0
18	6079.0
19	5901.5
20	5724.0
21	5320.0
22	4916.0
23	5125.0
24	5334.0
25	5541.5
26	6745.5
27	7742.0
28	9558.5
29	11375.0
30	13431.5
31	15488.0
32	19606.5
33	23725.0
34	26605.5
35	29486.0
36	32846.5
37	36207.0
38	43207.0
39	50207.0
40	71445.5
41	92684.0
42	112565.5
43	132447.0
44	131838.5
45	131230.0
46	133745.5
47	136261.0
48	128945.0
49	121629.0
50	117890.0
51	114151.0
52	103495.5
53	92840.0
54	84564.5
55	76289.0
56	68434.0
57	60579.0
58	62588.0
59	64597.0
60	58696.5
61	52796.0
62	44361.5
63	35927.0
64	30704.0
65	25481.0
66	21022.5
67	16564.0
68	13487.0
69	10410.0
70	8339.5
71	6269.0
72	5143.5
73	4018.0
74	3440.0
75	2034.0
76	1206.0
77	918.5
78	631.0
79	464.5
80	298.0
81	244.5
82	191.0
83	195.5
84	200.0
85	124.0
86	48.0
87	28.0
88	8.0
89	8.0
90	8.0
91	5.0
92	2.0
93	2.5
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1443113.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.81116704974417
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.31943941170813	17.736718774003158
2	7.304786848442226	3.1865185242829526
3	2.487694384592835	1.6277855337319453
4	1.2701590892852364	1.1081460830460486
5	0.7707783203344445	0.8405787351567896
6	0.525993731900776	0.6883522292163706
7	0.40602171020026184	0.6199065142900511
8	0.33595525422972905	0.5862060936995113
9	0.25942407084836355	0.509250757139849
>10	3.1271257357109263	15.799451044315335
>50	1.056700483966734	16.573425245400422
>100	1.1295322019818805	39.04328413617528
>500	0.004791567598904805	0.6410623852773261
>1k	0.001277751359707948	0.5341141447164419
>5k	3.19437839926987E-4	0.5051997995485156
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7251	0.5024554556711776	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4400	0.30489642876198886	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11024777685461915	0.0
2	0.0	0.0	0.0	0.3942172234606715	0.0
3	0.0	0.0	0.0	0.5459724914126614	0.0
4	0.0	0.0	0.0	0.8053423397890532	0.0
5	0.0	0.0	0.0	1.3260915811859502	0.0
6	0.0	0.0	0.0	1.8153117600631412	0.0
7	0.0	0.0	0.0	2.1621314477799034	0.0
8	0.0	0.0	0.0	3.0845124394278205	0.0
9	0.0	0.0	0.0	3.4706221896691387	0.0
10	0.0	0.0	0.0	4.1051532347085775	0.0
11	0.0	0.0	0.0	4.542956788553633	0.0
12	0.0	0.0	0.0	5.028227172785499	0.0
13	0.0	0.0	0.0	5.200978717536326	0.0
14	0.0	0.0	0.0	5.264106137218638	0.0
15	0.0	0.0	0.0	5.35945556584966	0.0
16	0.0	0.0	0.0	5.573506717769156	0.0
17	0.0	0.0	0.0	5.82858029828572	0.0
18	0.0	0.0	0.0	6.262780530699952	0.0
19	0.0	0.0	0.0	6.416545343296055	0.0
20	0.0	0.0	0.0	6.596988593408832	0.0
21	0.0	0.0	0.0	6.7920530131736045	0.0
22	0.0	0.0	0.0	6.995502084729332	0.0
23	0.0	0.0	0.0	7.23138104916247	0.0
24	0.0	0.0	0.0	7.412586540347152	0.0
25	0.0	0.0	0.0	7.557135165437495	0.0
26	0.0	0.0	0.0	7.69745681731091	0.0
27	0.0	0.0	0.0	7.833274317395796	0.0
28	0.0	0.0	0.0	7.975882692484927	0.0
29	0.0	0.0	0.0	8.12819231758012	0.0
30	0.0	0.0	0.0	8.340857576641607	0.0
31	0.0	0.0	0.0	8.517351032109058	0.0
32	0.0	0.0	0.0	8.67998555899642	0.0
33	0.0	0.0	0.0	8.850588969817332	0.0
34	0.0	0.0	0.0	9.025350059212272	0.0
35	0.0	0.0	0.0	9.240787103989778	0.0
36	0.0	0.0	0.0	9.413330764811903	0.0
37	0.0	0.0	0.0	9.586567372063033	0.0
38	0.0	0.0	0.0	9.764308131102693	0.0
39	0.0	0.0	0.0	9.947453872288587	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACACT	25	3.8913022E-5	45.000004	11
CGTAGTG	25	3.8913022E-5	45.000004	13
TTGCGAA	25	3.8913022E-5	45.000004	1
ACCGTTC	50	2.1827873E-11	45.000004	15
GCTACGT	25	3.8913022E-5	45.000004	21
CAATCGA	25	3.8913022E-5	45.000004	45
TTTTACG	55	1.8189894E-12	45.000004	1
TACCGTT	50	2.1827873E-11	45.000004	14
TATCACG	20	7.0338865E-4	45.0	1
CGTGATA	20	7.0338865E-4	45.0	2
CGTTATG	20	7.0338865E-4	45.0	3
ACCCGGT	20	7.0338865E-4	45.0	15
TATCGTT	20	7.0338865E-4	45.0	9
CCCATCG	20	7.0338865E-4	45.0	18
AATCGCG	40	6.8175723E-9	45.0	1
TACGTAG	70	0.0	45.0	1
GCACGAC	30	2.1658907E-6	44.999996	15
CTACCGT	30	2.1658907E-6	44.999996	35
GTTCACG	30	2.1658907E-6	44.999996	1
CGTCGAA	30	2.1658907E-6	44.999996	38
>>END_MODULE