FastQCFastQC Report
Thu 26 May 2016
SRR1546355_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546355_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2431174
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213020.8762021969632778No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT162950.044.0886761
CGGTCTA4500.042.031
TATTACG650.041.538461
AATACGG1500.040.52
TCACGAC4950.040.45454425
TACGGGA8900.040.196634
ACGGGAT10350.038.260875
GGGCGAT45350.038.153257
GCGCGAC10200.037.9411779
AGGGCGA22650.037.8476836
TAATGCG1250.037.81
CGATGTA1550.037.74193610
GTTTTTT197300.037.6900672
CGTAAGG3600.037.52
AATGCGG3250.037.3846172
GCGTAAG1750.037.2857171
AACGGGA8800.037.0738644
TAGGGAC24750.036.9090925
TTAGGGA29700.036.893944
TAGGGAT33400.036.8488045
Produced by FastQC (version 0.11.5)