FastQCFastQC Report
Thu 26 May 2016
SRR1546354_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546354_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2007562
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92100.4587654079923808No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC49990.24900849886578844No Hit
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT26280.13090504801346112TruSeq Adapter, Index 10 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC21640.10779243679647253RNA PCR Primer, Index 35 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAA302.1663145E-645.0000041
CGAACGT750.045.00000438
CTATACG650.045.0000041
GTCCGTT302.1663145E-645.00000410
TATAGCG750.045.0000041
ACGTACA302.1663145E-645.00000421
CCGAACG750.045.00000437
GACGAAC302.1663145E-645.00000438
TAGTCCG600.045.0000041
TACGTAG650.045.0000041
ACGCGCC302.1663145E-645.00000418
CGGAATA351.2125383E-745.019
GCCCATA406.8193913E-945.010
GTCGCAA207.034548E-445.011
ACACGTA351.2125383E-745.019
CGAACTA253.8918508E-545.033
GTCGACG453.8562575E-1045.011
CTCCGAT502.1827873E-1145.029
CTATCGA207.034548E-445.038
TTCGTAA253.8918508E-545.044
Produced by FastQC (version 0.11.5)