##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546354_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2007562
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07657746062139	31.0	31.0	33.0	30.0	34.0
2	31.39473699940525	31.0	31.0	34.0	30.0	34.0
3	31.473016026404167	31.0	31.0	34.0	30.0	34.0
4	35.31655560326406	37.0	35.0	37.0	33.0	37.0
5	35.217765130043304	37.0	35.0	37.0	32.0	37.0
6	35.25783114045793	37.0	35.0	37.0	32.0	37.0
7	35.737484570837665	37.0	35.0	37.0	35.0	37.0
8	35.64775085402095	37.0	35.0	37.0	35.0	37.0
9	37.40150740051864	39.0	37.0	39.0	35.0	39.0
10	36.873416611790816	39.0	37.0	39.0	32.0	39.0
11	36.568827762231	39.0	35.0	39.0	32.0	39.0
12	35.67726725251823	37.0	35.0	39.0	31.0	39.0
13	35.27572199513639	37.0	35.0	39.0	30.0	39.0
14	36.12735995202141	38.0	35.0	40.0	30.0	41.0
15	36.47746072101385	38.0	35.0	40.0	31.0	41.0
16	36.605112569375194	38.0	35.0	40.0	31.0	41.0
17	36.54314486924937	38.0	35.0	40.0	31.0	41.0
18	36.45683769666889	38.0	35.0	40.0	31.0	41.0
19	36.340177289667764	37.0	35.0	40.0	31.0	41.0
20	36.17762490025215	37.0	35.0	40.0	31.0	41.0
21	36.01171171799427	37.0	34.0	40.0	30.0	41.0
22	35.871626380654746	37.0	34.0	40.0	30.0	41.0
23	35.82639639522964	36.0	34.0	40.0	30.0	41.0
24	35.81880709039123	37.0	34.0	40.0	30.0	41.0
25	35.736465424230985	36.0	34.0	40.0	30.0	41.0
26	35.620061049173074	36.0	34.0	40.0	30.0	41.0
27	35.48127380374803	36.0	34.0	40.0	29.0	41.0
28	35.43283096611711	36.0	34.0	40.0	29.0	41.0
29	35.36516580807965	36.0	34.0	40.0	29.0	41.0
30	35.213946069909674	36.0	34.0	40.0	29.0	41.0
31	34.96432787629971	36.0	34.0	40.0	27.0	41.0
32	34.72534048761632	36.0	34.0	40.0	26.0	41.0
33	34.538463071128064	36.0	33.0	40.0	25.0	41.0
34	34.332873903769844	36.0	33.0	40.0	24.0	41.0
35	34.17301134410793	35.0	33.0	40.0	23.0	41.0
36	33.95889442019723	35.0	33.0	40.0	23.0	41.0
37	33.80392236952084	35.0	33.0	40.0	23.0	41.0
38	33.7515329539013	35.0	33.0	40.0	23.0	41.0
39	33.714904944405205	35.0	33.0	40.0	23.0	41.0
40	33.43688712976237	35.0	32.0	40.0	22.0	41.0
41	33.367530865796425	35.0	32.0	40.0	21.0	41.0
42	33.230532357157585	35.0	32.0	39.0	21.0	41.0
43	33.14783304326342	35.0	32.0	39.0	21.0	41.0
44	32.936481662832826	35.0	32.0	39.0	20.0	41.0
45	32.918617208335284	35.0	31.0	39.0	20.0	41.0
46	32.83747251641543	35.0	31.0	39.0	20.0	41.0
47	32.77372056255299	35.0	31.0	39.0	20.0	41.0
48	32.737074620858536	35.0	31.0	39.0	20.0	41.0
49	32.738014068805846	35.0	31.0	39.0	20.0	40.0
50	32.545404326242476	35.0	31.0	39.0	18.0	40.0
51	32.29962312496451	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	5.0
13	4.0
14	29.0
15	80.0
16	262.0
17	657.0
18	1560.0
19	3065.0
20	5262.0
21	8251.0
22	12168.0
23	17218.0
24	23818.0
25	32617.0
26	41302.0
27	48027.0
28	51830.0
29	58157.0
30	66466.0
31	78190.0
32	96436.0
33	122752.0
34	206030.0
35	279990.0
36	141695.0
37	171016.0
38	232347.0
39	308265.0
40	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.25961439796131	22.97174383655399	28.537599336907153	14.231042428577547
2	31.20566139426827	25.447732124835998	30.17724981843649	13.169356662459242
3	29.103509630088638	24.803717145472966	32.68691079030187	13.405862434136528
4	25.63457566939402	27.670726981283767	31.67025476672701	15.024442582595206
5	22.52558077907432	31.920508557145432	30.554373912237832	14.999536751542419
6	19.378280720595427	40.92546083259197	28.44709154686132	11.249166899951284
7	85.03144610228726	4.81115900779154	7.888772550984727	2.2686223389364812
8	85.89587768646747	3.481586122869431	7.514388098599197	3.108148092063906
9	81.80444738443944	4.8707337556698125	9.857478872383519	3.467339987507235
10	51.23428317531413	21.94477679892327	14.211615880356373	12.60932414540622
11	44.6660177867483	20.734154163109284	20.275239320130588	14.324588730011826
12	40.322241604493406	19.98777621811929	24.916241690169468	14.773740487217829
13	23.01084599130687	36.36749450328309	25.81608936610675	14.805570139303295
14	15.953828574161097	40.29245423055428	27.40413496569471	16.34958222958992
15	14.475219196219097	22.759097851025274	47.388773049101346	15.376909903654285
16	15.811715902173882	17.270898731894707	45.758636595034176	21.158748770897237
17	16.458819204587456	18.271465588609466	29.370699385622963	35.89901582118012
18	21.872948382167028	22.67441802544579	33.66381710751648	21.788816484870704
19	27.98468988753523	25.42910256320851	25.6049377304412	20.98126981881506
20	30.573800460459005	22.44040283687378	24.50703888597214	22.478757816695076
21	23.28112406989174	28.029470571768144	26.807241818683558	21.882163539656556
22	22.271142809039024	24.25175411768105	25.100843709932747	28.376259363347184
23	20.142291993970794	29.95170261242243	24.17354980817529	25.732455585431484
24	21.439088805227435	23.113607450230678	36.09940813783086	19.347895606711027
25	18.670257755426732	23.67114938417842	33.607231059364544	24.051361801030303
26	17.584512956511432	31.148477606171067	27.235323242818904	24.0316861944986
27	19.166830214957248	30.258044334371743	28.47837327066362	22.096752180007392
28	16.098730699226227	27.01137000999222	37.105703335687764	19.78419595509379
29	16.892479534878625	23.986706263617265	35.91450724809495	23.206306953409158
30	20.28027029800325	27.033287141318674	31.645847052295274	21.040595508382804
31	28.71722019045987	24.30589939438981	24.02177367373959	22.955106741410727
32	28.5987182463107	25.40713562021995	26.15142147540151	19.842724658067844
33	25.87058332445025	26.163625332617375	26.782684669265507	21.183106673666867
34	19.312878008250802	25.769664897024352	29.069936569829473	25.847520524895373
35	19.48263615270662	23.552149323408194	31.76903129268237	25.196183231202824
36	27.943495642973915	23.55703086629454	27.229196408379913	21.27027708235163
37	20.46885725073497	29.306741211479398	28.45416480288031	21.770236734905325
38	20.312697690033982	30.271593106464458	24.930936130490615	24.484773073010945
39	21.186742925000573	27.81408494482362	26.290346201013964	24.70882592916184
40	23.365006908877533	23.87104358420811	26.41432742799475	26.349622078919605
41	17.711632318204867	21.622594968424387	27.749230160762156	32.91654255260858
42	22.298489411534987	24.548033883885033	23.621337722072845	29.53213898250714
43	22.487972974184608	25.03962517720499	25.63253339124769	26.83986845736271
44	21.061865088101886	27.54550046275034	27.259432087278	24.13320236186977
45	18.36675529821744	32.000854768121734	24.799134472559253	24.833255461101576
46	22.195030589341698	29.65148772491211	26.954933396826597	21.198548288919593
47	21.936159381379007	25.257800257227426	27.41803241942217	25.38800794197141
48	23.002627067059446	22.29111728554336	30.098148899012834	24.60810674838436
49	21.072026667171425	21.664885069552025	31.45750915787408	25.805579105402472
50	18.884696960791246	27.87644914577981	28.95596748693191	24.282886406497035
51	18.895954396427108	29.727052016326272	25.98285881083623	25.394134776410393
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1197.0
1	1787.5
2	2378.0
3	9964.0
4	17550.0
5	12860.5
6	8171.0
7	8580.5
8	8990.0
9	9536.0
10	10082.0
11	10091.0
12	10100.0
13	9855.0
14	9610.0
15	9161.0
16	8712.0
17	8405.0
18	8098.0
19	7677.5
20	7257.0
21	6862.0
22	6467.0
23	6671.5
24	6876.0
25	7430.0
26	9141.0
27	10298.0
28	11995.5
29	13693.0
30	17028.5
31	20364.0
32	25206.5
33	30049.0
34	35417.5
35	40786.0
36	39288.5
37	37791.0
38	49479.0
39	61167.0
40	89801.5
41	118436.0
42	142596.5
43	166757.0
44	178450.5
45	190144.0
46	184137.5
47	178131.0
48	177497.0
49	176863.0
50	169412.0
51	161961.0
52	146650.5
53	131340.0
54	120090.0
55	108840.0
56	102299.5
57	95759.0
58	95346.0
59	94933.0
60	86150.5
61	77368.0
62	70107.0
63	62846.0
64	53549.0
65	44252.0
66	36158.5
67	28065.0
68	23010.0
69	17955.0
70	14087.0
71	10219.0
72	8705.5
73	7192.0
74	5839.5
75	3375.5
76	2264.0
77	1669.5
78	1075.0
79	765.0
80	455.0
81	399.5
82	344.0
83	226.0
84	108.0
85	90.5
86	73.0
87	63.0
88	53.0
89	32.5
90	12.0
91	9.0
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2007562.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.431251465911775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.38047871331491	16.627050250080174
2	7.677234367254004	3.1371101184021333
3	2.762647789682757	1.6933305510826127
4	1.2604330470281173	1.0300889815910745
5	0.8014755156605534	0.8187573902116039
6	0.51922860174248	0.636509407829662
7	0.3882832978903812	0.5553179589448337
8	0.3197872688574182	0.5226923284498439
9	0.2512002310818841	0.4619101580576209
>10	2.5076449650319863	11.584913344509571
>50	0.8356805522887358	12.414022393243574
>100	1.2804665321750708	47.259496311367
>500	0.011763137517515839	1.5530187386778147
>1k	0.0034309151092754534	1.24463809543325
>5k	2.450653649482467E-4	0.4611439721192466
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9210	0.4587654079923808	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4999	0.24900849886578844	No Hit
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT	2628	0.13090504801346112	TruSeq Adapter, Index 10 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	2164	0.10779243679647253	RNA PCR Primer, Index 35 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1779272570411275	0.0
2	0.0	0.0	0.0	0.5914636758416427	0.0
3	0.0	0.0	0.0	0.8081942176630161	0.0
4	0.0	0.0	0.0	1.1597649288041914	0.0
5	0.0	0.0	0.0	1.825049487886302	0.0
6	0.0	0.0	0.0	2.4620908345545494	0.0
7	0.0	0.0	0.0	2.859587898157068	0.0
8	0.0	0.0	0.0	3.9953436058263705	0.0
9	0.0	0.0	0.0	4.43767116532391	0.0
10	0.0	0.0	0.0	5.1962529675297695	0.0
11	0.0	0.0	0.0	5.723011294296266	0.0
12	0.0	0.0	0.0	6.2779132101524135	0.0
13	0.0	0.0	0.0	6.496088290174849	0.0
14	0.0	0.0	0.0	6.566571792054243	0.0
15	0.0	0.0	0.0	6.693392283775046	0.0
16	0.0	0.0	0.0	6.958739007811465	0.0
17	0.0	0.0	0.0	7.271356999186078	0.0
18	0.0	0.0	0.0	7.854004010835033	0.0
19	0.0	0.0	0.0	8.028643698177191	0.0
20	0.0	0.0	0.0	8.250654276181757	0.0
21	4.981166210557881E-5	0.0	0.0	8.456326629015692	0.0
22	4.981166210557881E-5	0.0	0.0	8.66105256026962	0.0
23	4.981166210557881E-5	0.0	0.0	8.904781022952218	0.0
24	4.981166210557881E-5	0.0	0.0	9.075236530677508	0.0
25	4.981166210557881E-5	0.0	0.0	9.203152878964636	0.0
26	4.981166210557881E-5	0.0	0.0	9.335602088503368	0.0
27	4.981166210557881E-5	0.0	0.0	9.472534347631605	0.0
28	4.981166210557881E-5	0.0	0.0	9.610761709974586	0.0
29	4.981166210557881E-5	0.0	0.0	9.759300086373422	0.0
30	4.981166210557881E-5	0.0	0.0	9.953416133598862	0.0
31	4.981166210557881E-5	0.0	0.0	10.132190188895786	0.0
32	4.981166210557881E-5	0.0	0.0	10.288100691286246	0.0
33	4.981166210557881E-5	0.0	0.0	10.449241418197794	0.0
34	4.981166210557881E-5	0.0	0.0	10.606546647127212	0.0
35	4.981166210557881E-5	0.0	0.0	10.817548847806444	0.0
36	4.981166210557881E-5	0.0	0.0	10.989996822015957	0.0
37	4.981166210557881E-5	0.0	0.0	11.16189686794231	0.0
38	4.981166210557881E-5	0.0	0.0	11.323436088150702	0.0
39	4.981166210557881E-5	0.0	0.0	11.490454591190709	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAAA	30	2.1663145E-6	45.000004	1
CGAACGT	75	0.0	45.000004	38
CTATACG	65	0.0	45.000004	1
GTCCGTT	30	2.1663145E-6	45.000004	10
TATAGCG	75	0.0	45.000004	1
ACGTACA	30	2.1663145E-6	45.000004	21
CCGAACG	75	0.0	45.000004	37
GACGAAC	30	2.1663145E-6	45.000004	38
TAGTCCG	60	0.0	45.000004	1
TACGTAG	65	0.0	45.000004	1
ACGCGCC	30	2.1663145E-6	45.000004	18
CGGAATA	35	1.2125383E-7	45.0	19
GCCCATA	40	6.8193913E-9	45.0	10
GTCGCAA	20	7.034548E-4	45.0	11
ACACGTA	35	1.2125383E-7	45.0	19
CGAACTA	25	3.8918508E-5	45.0	33
GTCGACG	45	3.8562575E-10	45.0	11
CTCCGAT	50	2.1827873E-11	45.0	29
CTATCGA	20	7.034548E-4	45.0	38
TTCGTAA	25	3.8918508E-5	45.0	44
>>END_MODULE