FastQCFastQC Report
Thu 26 May 2016
SRR1546353_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546353_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2269236
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144880.6384527656004048No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCG77490.3414805687905533No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC61840.2725146260679806No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGC45030.19843683072188176No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTC40680.17926738338365864No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG23390.10307433867610069No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGTA207.0347404E-445.00000429
AACTCGT207.0347404E-445.00000419
TCGCGAT207.0347404E-445.00000413
TATCCGT207.0347404E-445.00000433
CTATACG253.892013E-544.9999961
CCTAACG253.892013E-544.99999619
CGTTTTT109450.043.5198751
CGCATCG1150.043.0434821
TAGTACG1050.042.8571431
CGACGGT3800.042.03947428
CGGTCTA3800.042.03947431
CGTAAGG4000.041.6249962
TGCGTAG1950.041.538461
CGGGATC3350.040.970156
CGCAATA501.0822987E-940.49999638
TATAGCG1350.040.01
TTACGGG5750.039.9130443
CACGACG3900.039.80769326
ACGGGAT5500.039.681825
ACGCTAG403.460209E-739.3750041