##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546353_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2269236
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20062170704149	31.0	31.0	33.0	30.0	34.0
2	31.531261622854565	31.0	31.0	34.0	30.0	34.0
3	31.587961322665425	31.0	31.0	34.0	30.0	34.0
4	35.45634874468764	37.0	35.0	37.0	33.0	37.0
5	35.36369641588623	37.0	35.0	37.0	33.0	37.0
6	35.41305796312063	37.0	35.0	37.0	33.0	37.0
7	35.79374908559533	37.0	35.0	37.0	35.0	37.0
8	35.697527714173404	37.0	35.0	37.0	35.0	37.0
9	37.4700608486733	39.0	37.0	39.0	35.0	39.0
10	36.986500302304385	39.0	37.0	39.0	33.0	39.0
11	36.72791327125076	39.0	35.0	39.0	32.0	39.0
12	36.08333157062553	37.0	35.0	39.0	32.0	39.0
13	35.78151589345489	37.0	35.0	39.0	31.0	39.0
14	36.67473149553418	39.0	35.0	41.0	31.0	41.0
15	36.93130507360186	39.0	35.0	41.0	31.0	41.0
16	37.04847226114869	38.0	35.0	41.0	32.0	41.0
17	36.934310490402936	38.0	35.0	41.0	32.0	41.0
18	36.87027484140037	38.0	35.0	40.0	32.0	41.0
19	36.76268797075315	38.0	35.0	40.0	31.0	41.0
20	36.51009018013111	38.0	35.0	40.0	31.0	41.0
21	36.432811748094956	38.0	35.0	40.0	31.0	41.0
22	36.32506667442258	38.0	35.0	40.0	31.0	41.0
23	36.25700500080203	38.0	35.0	40.0	31.0	41.0
24	36.27938301701542	38.0	35.0	40.0	31.0	41.0
25	36.259548147482235	38.0	35.0	40.0	31.0	41.0
26	36.12633503082095	38.0	35.0	40.0	30.0	41.0
27	35.983382953557935	38.0	35.0	40.0	30.0	41.0
28	35.949568489130264	38.0	35.0	40.0	30.0	41.0
29	35.86587247866683	37.0	35.0	40.0	30.0	41.0
30	35.71818312418805	37.0	34.0	40.0	30.0	41.0
31	35.50000749150816	37.0	34.0	40.0	29.0	41.0
32	35.26532498162377	37.0	34.0	40.0	27.0	41.0
33	35.0516129657735	37.0	34.0	40.0	27.0	41.0
34	34.76672236823318	37.0	34.0	40.0	25.0	41.0
35	34.55540410957697	37.0	33.0	40.0	24.0	41.0
36	34.35083569976856	37.0	33.0	40.0	23.0	41.0
37	34.17409824275659	36.0	33.0	40.0	23.0	41.0
38	34.071662444981484	36.0	33.0	40.0	23.0	41.0
39	33.998177360133546	36.0	33.0	40.0	23.0	41.0
40	33.79063526226448	36.0	33.0	40.0	22.0	41.0
41	33.65882349830515	36.0	33.0	40.0	21.0	41.0
42	33.57680029754508	36.0	33.0	40.0	21.0	41.0
43	33.51893765126236	35.0	33.0	40.0	21.0	41.0
44	33.36480692180099	35.0	32.0	40.0	21.0	41.0
45	33.34315425984781	35.0	32.0	40.0	20.0	41.0
46	33.28473812331551	35.0	32.0	39.0	20.0	41.0
47	33.16913269488057	35.0	32.0	39.0	20.0	41.0
48	33.09520693308232	35.0	32.0	39.0	20.0	41.0
49	33.10838097051166	35.0	32.0	39.0	20.0	41.0
50	32.95252146537425	35.0	32.0	39.0	20.0	41.0
51	32.762735563863785	35.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	6.0
12	8.0
13	12.0
14	41.0
15	91.0
16	214.0
17	655.0
18	1592.0
19	3059.0
20	5490.0
21	8911.0
22	13135.0
23	18549.0
24	25312.0
25	34417.0
26	43655.0
27	49352.0
28	52102.0
29	57186.0
30	66026.0
31	79653.0
32	99142.0
33	128131.0
34	208331.0
35	276574.0
36	172924.0
37	216608.0
38	301907.0
39	406069.0
40	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.60506707984538	22.4814430936227	27.462414662908575	14.45107516362335
2	32.93148002235113	25.653832391166016	28.871347008420457	12.543340578062395
3	29.32824087049562	25.54846653234833	31.293836339631486	13.829456257524559
4	26.18550913170776	26.198509101741728	32.12195646464273	15.49402530190778
5	23.707141963198186	30.893305059500204	30.690417391580247	14.709135585721361
6	19.59619889689746	41.310203081565774	28.439219190952375	10.65437883058439
7	84.78959438330787	3.9448959914261894	9.493371337313528	1.772138287952421
8	85.81134796028267	4.397074610133102	7.836558207255657	1.9550192223285725
9	81.88310955757797	4.229088556677225	9.403208833281333	4.484593052463472
10	46.946769749818884	23.54920334420924	15.90284130870478	13.601185597267099
11	39.34588557558579	22.081925370477112	22.69649344537104	15.875695608566055
12	34.507517067418284	20.918009409334243	28.016125250965523	16.558348272281947
13	24.54786544898812	30.944291382650373	28.90999437696211	15.597848791399397
14	18.387554225298736	35.4705724746126	30.597654893541264	15.544218406547403
15	16.43213839371489	22.764798372668157	43.130331089406305	17.672732144210652
16	19.06474249483086	18.587224951481467	40.36697813713514	21.98105441655253
17	19.542568512045463	18.974007110763267	30.534594021952767	30.948830355238506
18	22.65546642129774	22.412521218595156	33.72663751147964	21.205374848627468
19	27.72135643890719	24.150286704423866	26.37892224519618	21.749434611472758
20	29.20612928756639	23.312603889591035	26.054055197432085	21.427211625410493
21	24.069246213262964	26.718463835405398	29.070268583787673	20.142021367543965
22	23.32683775508585	22.148511657668042	28.620117079052154	25.90453350819395
23	21.00596852861492	27.779393593262224	26.94999550509511	24.26464237302775
24	21.551526593091243	25.555649566638287	33.28547581653032	19.607348023740148
25	21.26323573220238	24.997664412163388	31.71380147327118	22.02529838236305
26	18.760983872986326	28.365802411031733	29.20515979827572	23.668053917706224
27	20.087333358011243	27.73647165830262	30.25842177719726	21.91777320648888
28	18.41051349440957	26.385884941010985	35.49679275315569	19.706808811423755
29	19.085233973019992	24.394509870282334	35.07938354582776	21.440872610869913
30	21.46123188597396	26.4476678494436	32.64402644766785	19.447073816914592
31	27.946718631292644	23.866270409952953	26.63557250105322	21.551438457701185
32	27.832847707334096	25.59288676893897	27.143188280108372	19.431077243618557
33	27.038130895155906	24.598587365968104	26.653111443675314	21.710170295200676
34	21.60793324273015	25.8006659510073	29.713833202011603	22.877567604250945
35	22.027898376369844	24.74189551020696	30.73906812689381	22.491137986529385
36	29.21956993455066	24.022490388835713	26.38883747657802	20.36910220003561
37	22.77079157919229	28.008633742810357	28.366243087982035	20.854331590015317
38	23.279685321403328	29.142715874417647	23.942463454660512	23.635135349518517
39	21.948708728400216	27.993959200365232	25.899333520180363	24.157998551054188
40	24.17505274903095	24.019273447098495	26.64676569559094	25.158908108279615
41	20.637121921210486	23.211777003361483	27.77467834989397	28.376422725534056
42	23.016733385156943	25.45517522196898	25.917225004362702	25.610866388511376
43	23.33441739863108	24.93689506071647	26.584498042512987	25.144189498139465
44	22.199806454683426	26.773372183413276	28.61504929412366	22.41177206777964
45	20.981334687092925	29.488074400370873	25.78074735285356	23.749843559682642
46	24.356391314081037	27.261950718215296	27.286937101297532	21.09472086640614
47	22.739723854195862	26.808097527097225	28.119331792726715	22.332846825980198
48	23.5703117701288	25.595090153690492	28.572744306894478	22.261853769286226
49	23.074682404121916	23.146644950106555	30.641854791656755	23.136817854114778
50	21.355689756376155	27.38873347681775	28.360822761493292	22.8947540053128
51	21.475201345298593	29.740009412859656	25.45645318512486	23.328336056716886
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	883.0
1	1890.0
2	2897.0
3	14209.5
4	25522.0
5	18440.5
6	11359.0
7	11537.5
8	11716.0
9	12096.0
10	12476.0
11	12316.0
12	12156.0
13	11574.0
14	10992.0
15	10633.0
16	10274.0
17	9539.0
18	8804.0
19	8279.0
20	7754.0
21	8079.5
22	8405.0
23	9515.0
24	10625.0
25	11692.5
26	14110.0
27	15460.0
28	18660.5
29	21861.0
30	24659.0
31	27457.0
32	31659.5
33	35862.0
34	43365.0
35	50868.0
36	51141.0
37	51414.0
38	63488.5
39	75563.0
40	96511.5
41	117460.0
42	136550.0
43	155640.0
44	167643.0
45	179646.0
46	195889.5
47	212133.0
48	196789.0
49	181445.0
50	179769.0
51	178093.0
52	163223.5
53	148354.0
54	136388.0
55	124422.0
56	120762.0
57	117102.0
58	112443.5
59	107785.0
60	97408.5
61	87032.0
62	78349.0
63	69666.0
64	63811.5
65	57957.0
66	48752.0
67	39547.0
68	33047.0
69	26547.0
70	21911.5
71	17276.0
72	14108.5
73	10941.0
74	8612.0
75	4752.0
76	3221.0
77	2594.0
78	1967.0
79	1428.5
80	890.0
81	651.0
82	412.0
83	270.0
84	128.0
85	107.0
86	86.0
87	68.0
88	50.0
89	34.5
90	19.0
91	15.5
92	12.0
93	12.0
94	12.0
95	6.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2269236.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.54430415897644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.70905936019868	15.774024045206076
2	6.842196587499834	2.6745194244321486
3	2.3821632602916103	1.396731699464346
4	1.242771466386696	0.9715641415663501
5	0.8030474488797038	0.7847501797497507
6	0.5893601730718303	0.6911180689021709
7	0.4816340111373528	0.6589241124883389
8	0.4018995389313531	0.6283877464181408
9	0.3416340776278657	0.6009300291807338
>10	3.7426935795930976	16.763383049725054
>50	1.186824285342385	16.846198876940637
>100	1.2635401364032237	38.31265604012864
>500	0.00817825322255483	1.152123119040904
>1k	0.004316300311903938	1.4828107366992591
>5k	4.543474012530461E-4	0.6186184985007868
>10k+	2.2717370062652305E-4	0.6432602315566927
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14488	0.6384527656004048	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCG	7749	0.3414805687905533	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6184	0.2725146260679806	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGC	4503	0.19843683072188176	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTC	4068	0.17926738338365864	No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	2339	0.10307433867610069	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.042657528789425164	0.0
2	0.0	0.0	0.0	0.18993176558101493	0.0
3	0.0	0.0	0.0	0.2791247803225403	0.0
4	0.0	0.0	0.0	0.45358878494788557	0.0
5	0.0	0.0	0.0	0.8967335261735668	0.0
6	0.0	0.0	0.0	1.1540888651510905	0.0
7	0.0	0.0	0.0	1.3691392168994323	0.0
8	0.0	0.0	0.0	1.8079653240121345	0.0
9	0.0	0.0	0.0	1.9949886217211432	0.0
10	0.0	0.0	0.0	2.661159967495668	0.0
11	0.0	0.0	0.0	2.9598948721067355	0.0
12	0.0	0.0	0.0	3.444286975880869	0.0
13	0.0	0.0	0.0	3.5740222700503606	0.0
14	0.0	0.0	0.0	3.6301645135190874	0.0
15	0.0	0.0	0.0	3.7455337391086694	0.0
16	0.0	0.0	0.0	3.917397749727221	0.0
17	0.0	0.0	0.0	4.095431237650028	0.0
18	0.0	0.0	0.0	4.367417051377644	0.0
19	0.0	0.0	0.0	4.525003128806347	0.0
20	0.0	0.0	0.0	4.656897740032328	0.0
21	0.0	0.0	0.0	4.793463526931531	0.0
22	0.0	0.0	0.0	4.9380496343262665	0.0
23	0.0	0.0	0.0	5.0973984195561854	0.0
24	0.0	0.0	0.0	5.210255786529035	0.0
25	0.0	0.0	0.0	5.30610302322015	0.0
26	0.0	0.0	0.0	5.405607878598788	0.0
27	0.0	0.0	0.0	5.534417751172642	0.0
28	0.0	0.0	0.0	5.6490378259467064	0.0
29	0.0	0.0	0.0	5.7712375442659996	0.0
30	0.0	0.0	0.0	5.91745415637686	0.0
31	0.0	0.0	0.0	6.038508114625363	0.0
32	0.0	0.0	0.0	6.155463777236039	0.0
33	0.0	0.0	0.0	6.281321114242855	0.0
34	0.0	0.0	0.0	6.410175054511739	0.0
35	4.4067695030397896E-5	0.0	0.0	6.5614153838560645	0.0
36	4.4067695030397896E-5	0.0	0.0	6.680882905083473	0.0
37	4.4067695030397896E-5	0.0	0.0	6.813879208685214	0.0
38	4.4067695030397896E-5	0.0	0.0	6.957672097569402	1.3220308509119367E-4
39	4.4067695030397896E-5	0.0	0.0	7.167522461304157	1.3220308509119367E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCGTA	20	7.0347404E-4	45.000004	29
AACTCGT	20	7.0347404E-4	45.000004	19
TCGCGAT	20	7.0347404E-4	45.000004	13
TATCCGT	20	7.0347404E-4	45.000004	33
CTATACG	25	3.892013E-5	44.999996	1
CCTAACG	25	3.892013E-5	44.999996	19
CGTTTTT	10945	0.0	43.519875	1
CGCATCG	115	0.0	43.04348	21
TAGTACG	105	0.0	42.857143	1
CGACGGT	380	0.0	42.039474	28
CGGTCTA	380	0.0	42.039474	31
CGTAAGG	400	0.0	41.624996	2
TGCGTAG	195	0.0	41.53846	1
CGGGATC	335	0.0	40.97015	6
CGCAATA	50	1.0822987E-9	40.499996	38
TATAGCG	135	0.0	40.0	1
TTACGGG	575	0.0	39.913044	3
CACGACG	390	0.0	39.807693	26
ACGGGAT	550	0.0	39.68182	5
ACGCTAG	40	3.460209E-7	39.375004	1
>>END_MODULE