FastQCFastQC Report
Thu 26 May 2016
SRR1546352_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546352_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences838979
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48910.5829704915140904No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC37190.44327688774093277No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGT453.8380676E-1045.00000419
TAAACGG600.045.0000042
CTAAACG302.1647975E-645.0000041
TATAGCG302.1647975E-645.0000041
TTACGAG750.045.0000041
ACGTATG600.045.0000041
AATCGTG302.1647975E-645.0000041
AACGTTC207.032197E-445.039
CGGCGAT207.032197E-445.020
CGAAAGG1100.045.02
ACGTTAG253.8899016E-545.01
TAGGTCG253.8899016E-545.010
ACCGACG207.032197E-445.015
GTCTATC253.8899016E-545.040
TATATCG207.032197E-445.027
CTAAGCG551.8189894E-1245.01
TCGGCCT207.032197E-445.015
ATGTACG351.2114106E-745.01
GTAGCGT207.032197E-445.01
TACGCGC253.8899016E-545.01
Produced by FastQC (version 0.11.5)