##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546352_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	838979
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29726012212463	31.0	31.0	33.0	30.0	34.0
2	31.63862146728345	31.0	31.0	34.0	30.0	34.0
3	31.668699693317713	31.0	31.0	34.0	30.0	34.0
4	35.52733977846883	37.0	35.0	37.0	33.0	37.0
5	35.46182800761402	37.0	35.0	37.0	33.0	37.0
6	35.51183641068489	37.0	35.0	37.0	33.0	37.0
7	35.91720412549063	37.0	35.0	37.0	35.0	37.0
8	35.843599184246564	37.0	35.0	37.0	35.0	37.0
9	37.63781691794431	39.0	37.0	39.0	35.0	39.0
10	37.155788166330744	39.0	37.0	39.0	33.0	39.0
11	36.84059314953056	39.0	37.0	39.0	32.0	39.0
12	35.95874866951378	37.0	35.0	39.0	32.0	39.0
13	35.584754803159555	37.0	35.0	39.0	31.0	39.0
14	36.4852159589215	38.0	35.0	41.0	31.0	41.0
15	36.800759017806165	38.0	35.0	41.0	32.0	41.0
16	36.93124857713959	38.0	35.0	41.0	32.0	41.0
17	36.8754033176039	38.0	35.0	41.0	32.0	41.0
18	36.76780348495016	38.0	35.0	40.0	32.0	41.0
19	36.63023508335727	37.0	35.0	40.0	32.0	41.0
20	36.47408099606784	37.0	35.0	40.0	32.0	41.0
21	36.32782107776237	37.0	35.0	40.0	31.0	41.0
22	36.2149052598456	37.0	35.0	40.0	31.0	41.0
23	36.168949401594084	37.0	35.0	40.0	31.0	41.0
24	36.1376601798138	37.0	35.0	40.0	31.0	41.0
25	36.082141507713544	37.0	35.0	40.0	31.0	41.0
26	35.96899445635707	36.0	35.0	40.0	31.0	41.0
27	35.86619927316417	36.0	34.0	40.0	30.0	41.0
28	35.85175791050789	36.0	35.0	40.0	30.0	41.0
29	35.83072758674532	36.0	35.0	40.0	30.0	41.0
30	35.70032384600807	36.0	35.0	40.0	30.0	41.0
31	35.44110162471289	36.0	34.0	40.0	30.0	41.0
32	35.18564946202468	36.0	34.0	40.0	29.0	41.0
33	34.94569470749566	36.0	34.0	40.0	27.0	41.0
34	34.67701813752192	36.0	34.0	40.0	25.0	41.0
35	34.48075219999547	36.0	34.0	40.0	24.0	41.0
36	34.26961103913209	36.0	33.0	40.0	23.0	41.0
37	34.06680381749722	35.0	33.0	40.0	23.0	41.0
38	34.02314599054327	35.0	33.0	40.0	23.0	41.0
39	33.98896873461672	35.0	33.0	40.0	23.0	41.0
40	33.75511425196578	35.0	33.0	40.0	23.0	41.0
41	33.712393278020066	35.0	33.0	40.0	22.0	41.0
42	33.61985222514509	35.0	33.0	40.0	22.0	41.0
43	33.55862423254933	35.0	33.0	40.0	22.0	41.0
44	33.38964980053136	35.0	33.0	40.0	22.0	41.0
45	33.3544296102763	35.0	33.0	39.0	22.0	41.0
46	33.25291574640128	35.0	32.0	39.0	21.0	41.0
47	33.22160983767174	35.0	32.0	39.0	21.0	41.0
48	33.16049388602099	35.0	32.0	39.0	20.0	41.0
49	33.1733213822992	35.0	32.0	39.0	20.0	41.0
50	33.035676697509714	35.0	32.0	39.0	20.0	41.0
51	32.83255480768887	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	2.0
13	1.0
14	11.0
15	28.0
16	86.0
17	181.0
18	443.0
19	878.0
20	1578.0
21	2608.0
22	3983.0
23	6119.0
24	8668.0
25	12600.0
26	16679.0
27	18750.0
28	19194.0
29	20973.0
30	23929.0
31	28531.0
32	36097.0
33	47475.0
34	86883.0
35	123792.0
36	57669.0
37	70908.0
38	102721.0
39	148164.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.270176011556906	23.017739418984267	29.28476159713175	15.42732297232708
2	32.10533279140479	25.52197373235802	29.423501660947416	12.949191815289776
3	28.030975745519253	25.250453229461044	33.6146673516262	13.103903673393495
4	24.99228228596902	26.801981932801656	32.939322676729695	15.266413104499636
5	22.21330927234174	32.50033671879749	31.406745580044316	13.879608428816454
6	19.5457812412468	40.642852800844835	29.26819384037026	10.543172117538104
7	86.0593650139038	4.242060885910136	8.21176692146049	1.48680717872557
8	87.82532101518632	3.2316661084484832	7.574683037358504	1.3683298390066974
9	84.13976988696976	4.592844397773961	9.078415550329627	2.188970164926655
10	51.49449509463289	24.52147193195539	14.022281844956785	9.961751128454942
11	45.161917044407545	19.67355559555126	22.505688461808937	12.658838898232256
12	40.50542385447073	20.979666952331346	25.719714081043744	12.795195112154179
13	22.834540554650353	38.546733589279356	25.408025707437254	13.210700148633043
14	15.948551751593307	41.078262983936426	28.705724457942335	14.267460806527934
15	14.765804626814258	22.222487094432637	48.63566310956532	14.376045169187787
16	16.542368760123914	18.038234568445695	45.785889754093965	19.63350691733643
17	17.545135217925598	17.93012697576459	29.97214471399165	34.55259309231816
18	22.495080329781793	22.57374737627521	33.9308850400308	21.000287253912195
19	28.111788256917038	23.58545327117842	26.748226117697822	21.55453235420672
20	30.306122084104608	22.48339946530247	25.736639415289297	21.473839035303623
21	22.725121844527692	26.535109937197475	28.965802481349357	21.773965736925476
22	22.541565402709722	24.039934253419933	26.995908121657397	26.422592222212955
23	20.1112304360419	28.54445701263083	26.164659663710292	25.17965288761697
24	20.787290265906535	23.46065872924114	37.937540748934126	17.814510255918208
25	17.987100988224974	23.762454125788608	35.9865979958974	22.263846890089027
26	17.3682535558101	32.15360575175302	29.04256244792778	21.4355782445091
27	17.647521570861727	31.363717089462313	31.000179980666974	19.988581359008986
28	15.512068836049531	28.220968582050325	38.64971590468891	17.617246677211227
29	16.454881469023658	23.2538597509592	38.706809109643984	21.584449670373157
30	19.527306404570314	28.252673785637068	32.70177203481851	19.518247774974107
31	28.321090277587402	25.394437763042937	26.088853237089367	20.195618722280294
32	28.199275548017294	28.03848487268454	26.786725293481723	16.975514285816452
33	28.25040912823801	26.84858619822427	25.48407051904756	19.41693415449016
34	19.85270191506581	27.429768802318055	29.5446012355494	23.172928047066733
35	20.72209197131275	25.347356727641575	31.19684759690052	22.733703704145157
36	29.078201003839187	24.62135524250309	27.125947133360906	19.174496620296814
37	21.503160388996626	30.97622228923489	28.447434321955615	19.073182999812868
38	22.096381435053797	31.06406715781921	24.52028000700852	22.319271400118478
39	21.675751121303392	29.960940619491073	27.046684124394055	21.31662413481148
40	23.353266291528154	25.848561167800387	25.84438943048634	24.953783110185118
41	18.477578103861955	24.404901672151507	27.58567258536864	29.53184763861789
42	22.339295739225893	26.126398872915768	24.123011422216766	27.41129396564157
43	23.502376102381586	26.24416105766652	25.629604555060375	24.62385828489152
44	20.73436879826551	29.874049290864253	27.278632719054947	22.11294919181529
45	18.93074796866191	34.224456154444866	23.57198451927879	23.272811357614433
46	22.683046893903185	31.81581422181008	26.18539915778583	19.3157397265009
47	22.891156989626676	26.75096754507562	27.327024871897866	23.030850593399833
48	23.942196407776596	23.80047653159376	30.08907255127959	22.168254509350056
49	21.498511881703834	23.651128335750954	31.288506625314817	23.561853157230395
50	19.337075183049873	30.990406196102644	27.480425612560026	22.192093008287454
51	19.49655474094107	32.34824709557689	24.36759442131448	23.787603742167562
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	376.0
1	640.0
2	904.0
3	4920.0
4	8936.0
5	6705.0
6	4474.0
7	4715.5
8	4957.0
9	5261.0
10	5565.0
11	5602.0
12	5639.0
13	5385.5
14	5132.0
15	4789.5
16	4447.0
17	4190.5
18	3934.0
19	3730.0
20	3526.0
21	3572.0
22	3618.0
23	3458.0
24	3298.0
25	3390.0
26	4412.0
27	5342.0
28	5851.0
29	6360.0
30	8713.5
31	11067.0
32	12752.0
33	14437.0
34	16278.0
35	18119.0
36	19027.5
37	19936.0
38	25551.0
39	31166.0
40	42887.0
41	54608.0
42	66931.0
43	79254.0
44	80746.0
45	82238.0
46	78631.0
47	75024.0
48	71266.5
49	67509.0
50	65283.5
51	63058.0
52	58456.5
53	53855.0
54	46408.0
55	38961.0
56	37565.5
57	36170.0
58	35017.5
59	33865.0
60	31125.5
61	28386.0
62	25182.5
63	21979.0
64	18035.0
65	14091.0
66	11771.0
67	9451.0
68	7410.0
69	5369.0
70	4747.5
71	4126.0
72	3610.0
73	3094.0
74	2399.5
75	1260.0
76	815.0
77	594.0
78	373.0
79	249.5
80	126.0
81	126.0
82	126.0
83	86.5
84	47.0
85	31.5
86	16.0
87	9.0
88	2.0
89	5.5
90	9.0
91	6.5
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	838979.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.334683431491893
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.98243854782974	17.06400006496702
2	6.064554524210373	2.587707018541005
3	2.1779129301731897	1.3939524871979372
4	1.2025014966752827	1.0261995502984944
5	0.8947871835911994	0.9545000650237226
6	0.6426653477652753	0.8226637048177077
7	0.5377888180760015	0.8031487930653374
8	0.43589204049765595	0.7439694955459627
9	0.37574273522994145	0.7214717077032309
>10	5.118172732176916	26.035443018543624
>50	1.978996562071706	30.71340176716737
>100	0.5863071308684425	15.969411009199424
>500	0.0011199754171316953	0.13548079993141274
>1k	0.0011199754171316953	1.0286505179977634
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4891	0.5829704915140904	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3719	0.44327688774093277	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.048868922821667764	0.0
2	0.0	0.0	0.0	0.17008768991834122	0.0
3	0.0	0.0	0.0	0.23576275449087522	0.0
4	0.0	0.0	0.0	0.3245611630326862	0.0
5	0.0	0.0	0.0	0.5007276701800641	0.0
6	0.0	0.0	0.0	0.6795164122105559	0.0
7	0.0	0.0	0.0	0.7799956852316924	0.0
8	0.0	0.0	0.0	1.04817879827743	0.0
9	0.0	0.0	0.0	1.1744036501509574	0.0
10	0.0	0.0	0.0	1.418271494280548	0.0
11	0.0	0.0	0.0	1.6843091424219199	0.0
12	0.0	0.0	0.0	1.8868171908951237	0.0
13	0.0	0.0	0.0	1.9789529892881705	0.0
14	0.0	0.0	0.0	2.011254155348346	0.0
15	0.0	0.0	0.0	2.0723999051227744	0.0
16	0.0	0.0	0.0	2.2099480439915657	0.0
17	0.0	0.0	0.0	2.3705003343349476	0.0
18	0.0	0.0	0.0	2.6343925175719534	0.0
19	0.0	0.0	0.0	2.734037443130281	0.0
20	0.0	0.0	0.0	2.8495349704819786	0.0
21	0.0	0.0	0.0	2.9633638029080585	0.0
22	0.0	0.0	0.0	3.086132072435663	0.0
23	0.0	0.0	0.0	3.2305933759963	0.0
24	0.0	0.0	0.0	3.3381050062039694	0.0
25	0.0	0.0	0.0	3.420109442548622	0.0
26	0.0	0.0	0.0	3.501756301409213	1.1919249468699455E-4
27	0.0	0.0	0.0	3.5960375647066254	1.1919249468699455E-4
28	0.0	0.0	0.0	3.6898420580252904	1.1919249468699455E-4
29	0.0	0.0	0.0	3.7946122608551587	1.1919249468699455E-4
30	0.0	0.0	0.0	3.9233401551171125	1.1919249468699455E-4
31	0.0	0.0	0.0	4.0363346400803835	1.1919249468699455E-4
32	0.0	0.0	0.0	4.142535152846496	1.1919249468699455E-4
33	0.0	0.0	0.0	4.251119515506348	1.1919249468699455E-4
34	0.0	0.0	0.0	4.3688817002570985	1.1919249468699455E-4
35	0.0	0.0	0.0	4.507144994094012	1.1919249468699455E-4
36	0.0	0.0	0.0	4.636588043324088	1.1919249468699455E-4
37	0.0	0.0	0.0	4.7620977402294935	1.1919249468699455E-4
38	0.0	0.0	0.0	4.879979117474931	1.1919249468699455E-4
39	0.0	0.0	0.0	5.015381791439356	1.1919249468699455E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGT	45	3.8380676E-10	45.000004	19
TAAACGG	60	0.0	45.000004	2
CTAAACG	30	2.1647975E-6	45.000004	1
TATAGCG	30	2.1647975E-6	45.000004	1
TTACGAG	75	0.0	45.000004	1
ACGTATG	60	0.0	45.000004	1
AATCGTG	30	2.1647975E-6	45.000004	1
AACGTTC	20	7.032197E-4	45.0	39
CGGCGAT	20	7.032197E-4	45.0	20
CGAAAGG	110	0.0	45.0	2
ACGTTAG	25	3.8899016E-5	45.0	1
TAGGTCG	25	3.8899016E-5	45.0	10
ACCGACG	20	7.032197E-4	45.0	15
GTCTATC	25	3.8899016E-5	45.0	40
TATATCG	20	7.032197E-4	45.0	27
CTAAGCG	55	1.8189894E-12	45.0	1
TCGGCCT	20	7.032197E-4	45.0	15
ATGTACG	35	1.2114106E-7	45.0	1
GTAGCGT	20	7.032197E-4	45.0	1
TACGCGC	25	3.8899016E-5	45.0	1
>>END_MODULE