FastQCFastQC Report
Thu 26 May 2016
SRR1546350_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546350_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2189777
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106790.48767522903017063No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC50620.23116509123988424No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT33910.15485595108543018No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTGA453.8562575E-1045.00000439
CGGTCTA1250.045.00000431
TCGCGAG650.045.0000041
ACGCGAA207.0346874E-445.027
GTCGACT406.8193913E-945.035
CGGCATA253.8919672E-545.026
TGAACGT351.2126111E-745.036
CGACCGG253.8919672E-545.032
CGCGATA253.8919672E-545.038
CGTAGAC253.8919672E-545.040
ACGTGCG253.8919672E-545.01
GTATCGA207.0346874E-445.029
CGTAACG207.0346874E-445.02
CACGTCA207.0346874E-445.044
GGTTACG351.2126111E-745.01
AATCGCG253.8919672E-545.01
CGTTTTT108400.043.484781
ACGGTCT1500.042.00000430
CGTAAGG4300.041.3372082
TTCGCGA603.6379788E-1241.24999614
Produced by FastQC (version 0.11.5)