##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546350_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2189777
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15181363216437	31.0	31.0	33.0	30.0	34.0
2	31.485227947868665	31.0	31.0	34.0	30.0	34.0
3	31.520736129752024	31.0	31.0	34.0	30.0	34.0
4	35.361466943894285	37.0	35.0	37.0	33.0	37.0
5	35.27538648912652	37.0	35.0	37.0	33.0	37.0
6	35.331163858237616	37.0	35.0	37.0	33.0	37.0
7	35.84433940077003	37.0	35.0	37.0	35.0	37.0
8	35.78622983070879	37.0	35.0	37.0	35.0	37.0
9	37.546499483737385	39.0	37.0	39.0	35.0	39.0
10	36.9747901270312	39.0	37.0	39.0	32.0	39.0
11	36.57242906469472	39.0	35.0	39.0	32.0	39.0
12	35.880868691195495	37.0	35.0	39.0	31.0	39.0
13	35.54373299198959	37.0	35.0	39.0	30.0	39.0
14	36.46975513945027	38.0	35.0	41.0	31.0	41.0
15	36.76435271719449	38.0	35.0	41.0	31.0	41.0
16	36.87008129138264	38.0	35.0	41.0	32.0	41.0
17	36.82520229228821	38.0	35.0	41.0	32.0	41.0
18	36.73299975294288	38.0	35.0	40.0	31.0	41.0
19	36.6244334468761	38.0	35.0	40.0	31.0	41.0
20	36.464537256533426	38.0	35.0	40.0	31.0	41.0
21	36.30467988292872	38.0	35.0	40.0	31.0	41.0
22	36.20746770104901	37.0	35.0	40.0	31.0	41.0
23	36.1701072757637	37.0	35.0	40.0	31.0	41.0
24	36.13328343479724	37.0	35.0	40.0	31.0	41.0
25	36.086897432934954	37.0	35.0	40.0	30.0	41.0
26	35.98813075486682	37.0	35.0	40.0	30.0	41.0
27	35.867173689375676	37.0	34.0	40.0	30.0	41.0
28	35.851237820106796	37.0	35.0	40.0	30.0	41.0
29	35.84083310766347	37.0	35.0	40.0	30.0	41.0
30	35.682479083486584	37.0	34.0	40.0	30.0	41.0
31	35.45704014609707	37.0	34.0	40.0	29.0	41.0
32	35.23118016126756	37.0	34.0	40.0	28.0	41.0
33	35.011407554285206	37.0	34.0	40.0	27.0	41.0
34	34.765324962313514	37.0	34.0	40.0	25.0	41.0
35	34.56043332266253	36.0	33.0	40.0	24.0	41.0
36	34.351646309190386	36.0	33.0	40.0	23.0	41.0
37	34.174773504334006	36.0	33.0	40.0	23.0	41.0
38	34.152085805997594	36.0	33.0	40.0	23.0	41.0
39	34.1362581669275	36.0	33.0	40.0	23.0	41.0
40	33.94811754804256	36.0	33.0	40.0	23.0	41.0
41	33.90434505431375	36.0	33.0	40.0	23.0	41.0
42	33.80172181916241	36.0	33.0	40.0	22.0	41.0
43	33.76579806984912	35.0	33.0	40.0	23.0	41.0
44	33.61999052871594	35.0	33.0	40.0	22.0	41.0
45	33.580386952644034	35.0	33.0	40.0	22.0	41.0
46	33.48733866507868	35.0	33.0	40.0	22.0	41.0
47	33.428831337620224	35.0	32.0	40.0	22.0	41.0
48	33.38130503699692	35.0	32.0	40.0	21.0	41.0
49	33.40952206548886	35.0	32.0	40.0	21.0	41.0
50	33.26376932445633	35.0	32.0	39.0	20.0	41.0
51	33.07104193714702	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	3.0
12	4.0
13	7.0
14	20.0
15	76.0
16	230.0
17	614.0
18	1272.0
19	2683.0
20	4502.0
21	7484.0
22	11254.0
23	16318.0
24	23882.0
25	33715.0
26	43893.0
27	49030.0
28	50778.0
29	55520.0
30	64942.0
31	77695.0
32	96228.0
33	123645.0
34	207097.0
35	287769.0
36	152715.0
37	188820.0
38	274574.0
39	414935.0
40	69.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.21026615952218	23.309953479281223	29.7103312346417	14.769449126554896
2	31.66993716711793	24.827779267021256	30.255957570108738	13.246325995752079
3	27.82333543552608	24.93820146983003	34.43076623784066	12.807696856803227
4	24.55213476075418	28.033722155269693	33.63648444567644	13.7776586382997
5	22.130426979550887	32.34137540032615	32.130806013580376	13.397391606542582
6	19.26465571608433	41.47408617407161	28.704886387974664	10.556371721869395
7	87.35990011768322	3.5799079084308585	7.729554196614541	1.3306377772713842
8	88.92942066703597	2.296626551470766	7.072044322321405	1.7019084591718698
9	84.53988693825902	4.528406317172936	8.728194697450927	2.2035120471171266
10	47.920404680476594	30.44004024154058	13.05219663920116	8.587358438781665
11	38.41687989233607	21.93428828597615	26.380722785927517	13.268109035760261
12	37.10898415683423	21.841310781874135	26.993296577688046	14.05640848360358
13	21.323723831239434	37.97341007782985	26.793139210065682	13.909726880865037
14	15.603552325191103	39.84232184373112	28.623188571256343	15.930937259821434
15	14.187152390403224	24.53560339705824	46.4505289808049	14.826715231733642
16	15.640999060634941	20.7838515063406	44.84095869122746	18.734190741796997
17	16.374224407325492	21.01451426332453	30.84793565737516	31.76332567197482
18	20.775448824241007	23.92563261007856	35.7098919205015	19.58902664517894
19	25.53291956212893	25.34472688314838	28.365354097700358	20.756999457022335
20	28.97678622069736	23.244604359256673	27.95677368060766	19.82183573943831
21	21.47615944454618	28.03111915048884	29.726588597834393	20.76613280713059
22	22.35971973401858	24.624607893863164	28.226253175551662	24.78941919656659
23	18.609383512567717	30.039771173046386	26.932377132466	24.418468181919895
24	19.3205061519963	25.16114654597249	38.07985927334153	17.43848802868968
25	16.943871453577238	26.66993031710535	35.23833705441239	21.147861174905024
26	17.373275908916753	32.25104656775553	29.177628589577843	21.198048933749874
27	17.728106560622383	31.862148520146118	30.32354436090981	20.086200558321693
28	14.520245668851212	28.84809731767207	38.26722995081234	18.364427062664372
29	16.077253528555644	23.65414377811074	37.75708668051587	22.511516012817744
30	18.881740012795824	28.583367164784356	33.50144786432591	19.033444958093906
31	25.595894011125335	26.896437399790024	27.04617867481483	20.46148991426981
32	26.323228346996064	27.014257616186487	27.504033515741554	19.15848052107589
33	25.8647798383123	28.640587603212563	26.65522562343106	18.83940693504407
34	19.810601718805156	27.088466085816044	30.621519908191562	22.479412287187234
35	20.091223900881232	26.974847210469378	31.098463450844537	21.835465437804853
36	27.289217121195446	25.81020807141549	28.15355170868997	18.747023098699092
37	20.555746087386982	30.63960394140591	30.255592236104405	18.549057735102707
38	20.564879437495236	30.6307445918009	27.603906699175308	21.20046927152856
39	20.275854573319567	31.265603757825566	28.29096296106864	20.167578707786227
40	22.925667773476476	27.159340882656085	27.6491624489617	22.265828894905738
41	17.88067917418075	25.71992490559541	28.75909282086715	27.640303099356693
42	21.828250091219335	26.535944071017276	25.83769945524133	25.798106382522057
43	21.634212068169496	28.274522930873786	27.14139384969337	22.94987115126335
44	19.905040558924494	30.350670410731322	28.198350790970956	21.545938239373232
45	18.4275385119124	34.014559473407566	24.71753973121464	22.840362283465392
46	21.815235067315072	31.530151243711117	27.204276965188694	19.450336723785117
47	20.994238226084207	28.262329908479266	28.193510115413577	22.549921750022946
48	21.966483345107743	24.47139594579722	30.50516102781242	23.056959681282617
49	20.73978309206828	24.5240497091713	31.41877003914097	23.31739715961945
50	18.67971030840127	30.807200915892345	29.039486669190516	21.47360210651587
51	18.653771594093826	31.49434851128677	26.01959012264719	23.832289771972214
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	763.0
1	1281.5
2	1800.0
3	10516.5
4	19233.0
5	14729.0
6	10225.0
7	10627.5
8	11030.0
9	11684.0
10	12338.0
11	12374.5
12	12411.0
13	12357.5
14	12304.0
15	11547.5
16	10791.0
17	10026.5
18	9262.0
19	9328.5
20	9395.0
21	9161.0
22	8927.0
23	9100.5
24	9274.0
25	11059.5
26	16066.5
27	19288.0
28	23480.5
29	27673.0
30	31452.0
31	35231.0
32	45665.5
33	56100.0
34	66745.0
35	77390.0
36	78590.0
37	79790.0
38	94862.0
39	109934.0
40	135198.0
41	160462.0
42	184688.5
43	208915.0
44	210168.5
45	211422.0
46	207724.0
47	204026.0
48	193249.0
49	182472.0
50	172047.0
51	161622.0
52	150019.0
53	138416.0
54	118851.5
55	99287.0
56	87364.5
57	75442.0
58	68313.5
59	61185.0
60	57014.5
61	52844.0
62	43617.0
63	34390.0
64	28632.0
65	22874.0
66	17147.5
67	11421.0
68	9524.0
69	7627.0
70	5873.0
71	4119.0
72	3419.5
73	2720.0
74	2344.0
75	1691.0
76	1414.0
77	1039.0
78	664.0
79	460.5
80	257.0
81	171.0
82	85.0
83	68.0
84	51.0
85	54.5
86	58.0
87	39.5
88	21.0
89	13.0
90	5.0
91	3.0
92	1.0
93	2.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2189777.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.928548352098904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.68475427126691	16.07930026772161
2	7.797405104458023	3.10781929290189
3	2.618118634975675	1.5652591142593177
4	1.300457797166379	1.0366494436277687
5	0.7836259629637872	0.7808263946441948
6	0.530765385895465	0.6346430193862924
7	0.4159837104266451	0.5802966040846035
8	0.3312969869039703	0.528181441793636
9	0.26697312014179553	0.4788348060110834
>10	3.0140418863736156	13.87277400836192
>50	0.9667935255606672	13.951402781500729
>100	1.27989051803057	45.25432575734575
>500	0.00805251986691897	0.9648675895410938
>1k	0.001380431977186109	0.4430944870426311
>5k	2.3007199619768485E-4	0.23209274559290138
>10k+	2.3007199619768485E-4	0.4896322461846293
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10679	0.48767522903017063	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5062	0.23116509123988424	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3391	0.15485595108543018	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08032781420208542	0.0
2	0.0	0.0	0.0	0.27468550450570994	0.0
3	0.0	0.0	0.0	0.38373770479825114	0.0
4	0.0	0.0	0.0	0.5514260127857769	0.0
5	0.0	0.0	0.0	0.8936526413420179	0.0
6	0.0	0.0	0.0	1.2341896001282322	0.0
7	0.0	0.0	0.0	1.4685056971554638	0.0
8	0.0	0.0	0.0	2.1575256293220724	0.0
9	0.0	0.0	0.0	2.464132192456127	0.0
10	0.0	0.0	0.0	2.9676994506746577	0.0
11	0.0	0.0	0.0	3.3467334801671584	0.0
12	0.0	0.0	0.0	3.7052631386666315	0.0
13	0.0	0.0	0.0	3.840756387522565	0.0
14	0.0	0.0	0.0	3.8914464806233693	0.0
15	0.0	0.0	0.0	3.9699932915543457	0.0
16	0.0	0.0	0.0	4.153664962231314	0.0
17	0.0	0.0	0.0	4.3925020675621305	0.0
18	0.0	0.0	0.0	4.749342056291577	0.0
19	0.0	0.0	0.0	4.891867984730865	0.0
20	0.0	0.0	0.0	5.054258949655604	0.0
21	0.0	0.0	0.0	5.243090963143736	0.0
22	0.0	0.0	0.0	5.4470386710610255	0.0
23	0.0	0.0	0.0	5.682770437355036	0.0
24	0.0	0.0	0.0	5.853518417628827	0.0
25	0.0	0.0	0.0	5.990610002753705	0.0
26	0.0	0.0	0.0	6.128021255132372	0.0
27	0.0	0.0	0.0	6.268857513801634	0.0
28	0.0	0.0	0.0	6.410926774735509	0.0
29	0.0	0.0	0.0	6.567472395590967	0.0
30	0.0	0.0	0.0	6.7874491329482405	0.0
31	0.0	0.0	0.0	6.9577404457166185	0.0
32	0.0	0.0	0.0	7.119948743639193	0.0
33	0.0	0.0	0.0	7.290833724164607	0.0
34	0.0	0.0	0.0	7.463088707206259	0.0
35	0.0	0.0	0.0	7.6866274511057515	0.0
36	0.0	0.0	0.0	7.870253455032179	0.0
37	0.0	0.0	0.0	8.05652813049	0.0
38	0.0	0.0	0.0	8.249972485782799	0.0
39	0.0	0.0	0.0	8.450586520910576	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTGA	45	3.8562575E-10	45.000004	39
CGGTCTA	125	0.0	45.000004	31
TCGCGAG	65	0.0	45.000004	1
ACGCGAA	20	7.0346874E-4	45.0	27
GTCGACT	40	6.8193913E-9	45.0	35
CGGCATA	25	3.8919672E-5	45.0	26
TGAACGT	35	1.2126111E-7	45.0	36
CGACCGG	25	3.8919672E-5	45.0	32
CGCGATA	25	3.8919672E-5	45.0	38
CGTAGAC	25	3.8919672E-5	45.0	40
ACGTGCG	25	3.8919672E-5	45.0	1
GTATCGA	20	7.0346874E-4	45.0	29
CGTAACG	20	7.0346874E-4	45.0	2
CACGTCA	20	7.0346874E-4	45.0	44
GGTTACG	35	1.2126111E-7	45.0	1
AATCGCG	25	3.8919672E-5	45.0	1
CGTTTTT	10840	0.0	43.48478	1
ACGGTCT	150	0.0	42.000004	30
CGTAAGG	430	0.0	41.337208	2
TTCGCGA	60	3.6379788E-12	41.249996	14
>>END_MODULE