FastQCFastQC Report
Thu 26 May 2016
SRR1546349_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546349_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1620473
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79590.4911528917791287No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC41400.2554809614229919No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG18360.11330025245715293No Hit
GTACAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG17560.10836342228472796No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA207.034142E-445.00000418
TACCCGA207.034142E-445.00000432
TTATACG302.1660544E-645.0000041
AATGCGC253.891517E-545.011
TATTACG351.2123382E-745.01
TCGACTA253.891517E-545.02
CAATACG253.891517E-545.01
TAATGCG253.891517E-545.01
CGTTTTT62000.043.11291
CGGTCTA2900.042.67241331
ACGTATA850.042.35294313
ATTTACG800.042.1875041
TACGACG700.041.7857131
TCGATAG1300.041.5384641
TCACGAC3100.041.37096825
CGACGGT3050.041.31147828
ACTTACG556.184564E-1140.9090921
TAATACG501.0822987E-940.51
GTTTACG501.0822987E-940.51
GTACGTA900.040.011
Produced by FastQC (version 0.11.5)