##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546349_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1620473
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.086543867130153	31.0	31.0	33.0	30.0	34.0
2	31.407142852734975	31.0	31.0	34.0	30.0	34.0
3	31.49429703549519	31.0	31.0	34.0	30.0	34.0
4	35.35256681228259	37.0	35.0	37.0	33.0	37.0
5	35.25455036893549	37.0	35.0	37.0	33.0	37.0
6	35.29962054289087	37.0	35.0	37.0	32.0	37.0
7	35.74527005386699	37.0	35.0	37.0	35.0	37.0
8	35.654918039362585	37.0	35.0	37.0	35.0	37.0
9	37.407232332781845	39.0	37.0	39.0	35.0	39.0
10	36.86635753881737	39.0	37.0	39.0	32.0	39.0
11	36.55168336652323	39.0	35.0	39.0	32.0	39.0
12	35.8883017489338	37.0	35.0	39.0	31.0	39.0
13	35.58057307958849	37.0	35.0	39.0	30.0	39.0
14	36.38073636524644	38.0	35.0	41.0	30.0	41.0
15	36.67480482550465	38.0	35.0	41.0	31.0	41.0
16	36.763584459599144	38.0	35.0	41.0	31.0	41.0
17	36.703004616553315	38.0	35.0	40.0	31.0	41.0
18	36.637428701373	38.0	35.0	40.0	31.0	41.0
19	36.525619988731684	38.0	35.0	40.0	31.0	41.0
20	36.34377925457567	38.0	35.0	40.0	31.0	41.0
21	36.2091636207453	38.0	35.0	40.0	30.0	41.0
22	36.120858539451135	38.0	34.0	40.0	30.0	41.0
23	36.066479355101876	38.0	34.0	40.0	30.0	41.0
24	36.05148620186822	38.0	34.0	40.0	30.0	41.0
25	35.96374823894011	37.0	34.0	40.0	30.0	41.0
26	35.859339834727265	37.0	34.0	40.0	30.0	41.0
27	35.74484980619856	37.0	34.0	40.0	30.0	41.0
28	35.68850576344067	37.0	34.0	40.0	29.0	41.0
29	35.58086743808752	37.0	34.0	40.0	29.0	41.0
30	35.380390787134374	37.0	34.0	40.0	29.0	41.0
31	35.150790540786545	37.0	34.0	40.0	27.0	41.0
32	34.99254415223209	37.0	34.0	40.0	27.0	41.0
33	34.77603576239777	37.0	33.0	40.0	25.0	41.0
34	34.50821334264749	36.0	33.0	40.0	24.0	41.0
35	34.31116902287172	36.0	33.0	40.0	23.0	41.0
36	34.108389340643136	36.0	33.0	40.0	23.0	41.0
37	33.897811318053435	36.0	33.0	40.0	23.0	41.0
38	33.78446416570964	35.0	33.0	40.0	23.0	41.0
39	33.71993547562965	36.0	33.0	40.0	22.0	41.0
40	33.46780045085602	35.0	32.0	40.0	21.0	41.0
41	33.36371726033078	35.0	32.0	40.0	21.0	41.0
42	33.24575540598332	35.0	32.0	40.0	20.0	41.0
43	33.193631735919084	35.0	32.0	40.0	21.0	41.0
44	33.0235783009035	35.0	31.0	39.0	20.0	41.0
45	32.99413998258533	35.0	31.0	39.0	20.0	41.0
46	32.916113381710154	35.0	31.0	39.0	20.0	41.0
47	32.82177981367169	35.0	31.0	39.0	20.0	41.0
48	32.71973059717749	35.0	31.0	39.0	20.0	41.0
49	32.694394167628836	35.0	31.0	39.0	20.0	41.0
50	32.552649751029485	35.0	31.0	39.0	19.0	40.0
51	32.35144121500328	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	4.0
12	5.0
13	12.0
14	39.0
15	102.0
16	233.0
17	637.0
18	1323.0
19	2590.0
20	4516.0
21	6823.0
22	10023.0
23	14232.0
24	19090.0
25	26111.0
26	33385.0
27	38185.0
28	41047.0
29	45661.0
30	52477.0
31	62353.0
32	74866.0
33	95472.0
34	152458.0
35	192775.0
36	125196.0
37	155604.0
38	205658.0
39	259550.0
40	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.524857865573814	22.87406207940521	28.903597900119287	13.697482154901685
2	32.07378339534198	24.659960394279942	30.609704697332198	12.656551513045883
3	29.916203478860805	25.30514238743873	31.23581818394999	13.542835949750472
4	26.099354941427595	27.13362086255063	31.055315330770703	15.711708865251072
5	22.455665722292196	32.6212778614639	30.243762160801197	14.679294255442702
6	20.837434502148447	41.299731621569755	27.511843764135534	10.350990112146269
7	86.77898366711447	4.227160835138876	7.279479509994921	1.7143759877517244
8	88.0031324187444	3.1218662699100816	6.882558364131953	1.9924429472135605
9	83.81583648724786	4.570332242499567	8.58952910662504	3.0243021636275333
10	48.402410901014704	24.25501689938678	14.345934798049706	12.996637401548808
11	40.13235641692272	22.250046745610696	21.69002507292624	15.927571764540355
12	36.75247906012627	19.767006300012405	27.58083596579517	15.899678674066154
13	25.418566060650193	29.927496477880222	27.472966226527685	17.180971234941897
14	19.34077272500066	33.92157721850349	29.69386098997021	17.04378906652564
15	17.7235288708914	22.56828716060064	42.36355681335018	17.344627155157784
16	21.74803282745223	17.48514168394043	39.34561081856964	21.4212146700377
17	21.031760479810522	18.523418779578556	29.248250356531702	31.196570384079216
18	24.886560899194247	21.58573453553376	32.23836497121519	21.2893395940568
19	29.264603606477863	24.14239546107834	25.215477209432063	21.377523723011738
20	30.035119375639084	23.73695828316794	24.314999385981746	21.912922955211226
21	25.769512975532454	25.01263519972255	28.5250664466486	20.692785378096396
22	24.681065343267058	22.334034568919076	26.531697843777714	26.453202244036156
23	22.886650996344894	26.78390815521147	25.61048533360321	24.71895551484042
24	23.062278729728913	24.536416219215006	32.44484789317687	19.95645715787921
25	22.733547550622564	22.71700916954494	30.497206679778067	24.052236600054428
26	20.69877128468046	27.34627482222783	26.383099255587723	25.57185463750399
27	20.657610463117866	27.192060589716704	29.079534185389083	23.070794761776344
28	18.694418234675926	26.07664552263444	33.405184782467835	21.8237514602218
29	20.725553588365866	24.482481349581263	32.1617206827883	22.63024437926457
30	23.134665002132092	25.763280227439765	30.458637694055994	20.643417076372145
31	28.80813194666002	24.359677699042194	25.216896548106632	21.615293806191154
32	29.72872735306296	24.767891843924584	25.438683643602822	20.06469715940963
33	28.15727259880294	24.791958891015152	25.574076211081582	21.476692299100325
34	22.325703668003108	25.484040770811976	29.239796034861428	22.95045952632349
35	22.432339199727487	25.46201016616753	28.959322370690533	23.146328263414446
36	29.552050543267306	25.05669640901144	25.151977231339245	20.23927581638201
37	23.297642108199273	29.10508228153138	26.296951569078903	21.300324041190443
38	23.681850916368248	29.31428046008789	23.764913084019295	23.23895553952457
39	23.284497797865193	26.72429593087944	25.27027602434598	24.72093024690939
40	25.01183296481953	23.94547764757574	25.385612719249256	25.657076668355472
41	21.429298729445044	22.854499889846977	26.231415148539966	29.484786232168013
42	23.23728935934138	24.886314057685627	24.407564951714715	27.46883163125828
43	22.497628778757807	25.669110191900764	25.977970629563096	25.855290399778337
44	22.516327023035867	26.31046614167592	26.715965029963474	24.457241805324742
45	20.93925662445471	29.097368484386966	24.947160489560762	25.01621440159756
46	23.34336949767136	27.286909439404422	26.878818715276342	22.49090234764788
47	23.15323982565584	26.06072424532837	27.316530420438973	23.469505508576816
48	23.23438897161508	24.767213029775874	28.47489591002133	23.523502088587716
49	23.069066871215995	22.797417790978315	30.112565898969006	24.020949438836688
50	21.93106580609489	26.59871531336838	27.792749400946516	23.67746947959022
51	21.60153239208552	28.346785167046907	25.731190831319005	24.32049160954857
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	716.0
1	1394.0
2	2072.0
3	8394.0
4	14716.0
5	10723.5
6	6731.0
7	6802.5
8	6874.0
9	7190.0
10	7506.0
11	7435.5
12	7365.0
13	7171.0
14	6977.0
15	6556.5
16	6136.0
17	5948.0
18	5760.0
19	5764.0
20	5768.0
21	5671.0
22	5574.0
23	5596.0
24	5618.0
25	5758.5
26	7016.5
27	8134.0
28	10382.5
29	12631.0
30	15162.0
31	17693.0
32	20079.0
33	22465.0
34	26667.5
35	30870.0
36	32880.5
37	34891.0
38	40189.0
39	45487.0
40	60598.0
41	75709.0
42	92610.5
43	109512.0
44	113420.0
45	117328.0
46	122885.5
47	128443.0
48	127511.5
49	126580.0
50	126032.5
51	125485.0
52	119662.5
53	113840.0
54	106560.5
55	99281.0
56	97821.0
57	96361.0
58	93865.0
59	91369.0
60	84217.5
61	77066.0
62	69616.5
63	62167.0
64	54393.0
65	46619.0
66	38823.0
67	31027.0
68	25941.0
69	20855.0
70	18762.5
71	16670.0
72	13517.0
73	10364.0
74	7776.0
75	4160.0
76	3132.0
77	2378.5
78	1625.0
79	1255.0
80	885.0
81	715.5
82	546.0
83	438.5
84	331.0
85	217.5
86	104.0
87	75.5
88	47.0
89	40.5
90	34.0
91	23.5
92	13.0
93	9.0
94	5.0
95	3.5
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1620473.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.81896661162019
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.69670051207719	18.414153146530033
2	6.450949807593914	2.944080165454465
3	2.2891574371160983	1.5670862137888288
4	1.3153722355900366	1.2006174050312501
5	0.8791552239129332	1.003070685045035
6	0.670533389296205	0.9180527413395976
7	0.505522104382381	0.8074844414936269
8	0.4135893432283502	0.7550145131239718
9	0.35190334909337445	0.7227063696131133
>10	4.279868231158276	22.799325016903325
>50	1.357960938780081	22.328600798055355
>100	0.7819515377378776	24.3105087328688
>500	0.004347194093807842	0.6891814181295918
>1k	0.0027169963086299013	1.046667929188896
>5k	2.7169963086299013E-4	0.49345042343409695
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7959	0.4911528917791287	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4140	0.2554809614229919	No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	1836	0.11330025245715293	No Hit
GTACAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1756	0.10836342228472796	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07022640920274513	0.0
2	0.0	0.0	0.0	0.21635658230652408	0.0
3	0.0	0.0	0.0	0.3079347820050072	0.0
4	0.0	0.0	0.0	0.43277487499020345	0.0
5	0.0	0.0	0.0	0.6893049128248357	0.0
6	0.0	0.0	0.0	0.9666313477608081	0.0
7	0.0	0.0	0.0	1.1496026160263084	0.0
8	0.0	0.0	0.0	1.6185397720295247	0.0
9	0.0	0.0	0.0	1.841499364691667	0.0
10	0.0	0.0	0.0	2.22373344079167	0.0
11	0.0	0.0	0.0	2.5376541293807424	0.0
12	0.0	0.0	0.0	2.8346661746292594	0.0
13	0.0	0.0	0.0	2.9564824591338454	0.0
14	0.0	0.0	0.0	3.0047399740692997	0.0
15	0.0	0.0	0.0	3.0808905794789547	0.0
16	0.0	0.0	0.0	3.2326981072810224	0.0
17	0.0	0.0	0.0	3.4280114509775848	0.0
18	0.0	0.0	0.0	3.7373655716571643	0.0
19	0.0	0.0	0.0	3.863069609922535	0.0
20	0.0	0.0	0.0	4.0054354500198395	0.0
21	0.0	0.0	0.0	4.1681657145783975	0.0
22	0.0	0.0	0.0	4.333672946108945	0.0
23	0.0	0.0	0.0	4.515903689848582	0.0
24	0.0	0.0	0.0	4.65413493467648	0.0
25	0.0	0.0	0.0	4.761387570172412	0.0
26	0.0	0.0	0.0	4.876354002812759	0.0
27	0.0	0.0	0.0	5.009956969354009	0.0
28	0.0	0.0	0.0	5.133007461401702	0.0
29	6.171037715531206E-5	0.0	0.0	5.277348033567977	0.0
30	6.171037715531206E-5	0.0	0.0	5.454950499020965	0.0
31	6.171037715531206E-5	0.0	0.0	5.6126822230299425	0.0
32	6.171037715531206E-5	0.0	0.0	5.758380423493635	0.0
33	6.171037715531206E-5	0.0	0.0	5.909941109787081	0.0
34	6.171037715531206E-5	0.0	0.0	6.069709276242183	0.0
35	6.171037715531206E-5	0.0	0.0	6.26471406805297	0.0
36	6.171037715531206E-5	0.0	0.0	6.420471059992978	0.0
37	6.171037715531206E-5	0.0	0.0	6.584435532094641	0.0
38	6.171037715531206E-5	0.0	0.0	6.7838217606834545	0.0
39	6.171037715531206E-5	0.0	0.0	7.1068755850915135	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	20	7.034142E-4	45.000004	18
TACCCGA	20	7.034142E-4	45.000004	32
TTATACG	30	2.1660544E-6	45.000004	1
AATGCGC	25	3.891517E-5	45.0	11
TATTACG	35	1.2123382E-7	45.0	1
TCGACTA	25	3.891517E-5	45.0	2
CAATACG	25	3.891517E-5	45.0	1
TAATGCG	25	3.891517E-5	45.0	1
CGTTTTT	6200	0.0	43.1129	1
CGGTCTA	290	0.0	42.672413	31
ACGTATA	85	0.0	42.352943	13
ATTTACG	80	0.0	42.187504	1
TACGACG	70	0.0	41.785713	1
TCGATAG	130	0.0	41.538464	1
TCACGAC	310	0.0	41.370968	25
CGACGGT	305	0.0	41.311478	28
ACTTACG	55	6.184564E-11	40.909092	1
TAATACG	50	1.0822987E-9	40.5	1
GTTTACG	50	1.0822987E-9	40.5	1
GTACGTA	90	0.0	40.0	11
>>END_MODULE