FastQCFastQC Report
Thu 26 May 2016
SRR1546348_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546348_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2049608
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97400.4752128211833678No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC44400.21662678912260294No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG20860.10177555903372743No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCAAC253.8918784E-545.00000415
CATACGT253.8918784E-545.00000410
ACCGGAT207.0345815E-445.06
TCACCGT207.0345815E-445.016
CGACGTA207.0345815E-445.011
ACGTATC207.0345815E-445.034
CGTAGCA302.1663382E-644.99999617
TAGCCGT1350.043.33333644
CGTTTTT80950.042.9709661
TGCAACG1100.042.9545481
CCGTAGG850.042.352942
TACGTAG700.041.7857131
GCGCGAC11200.040.5803579
TCGTTAG1000.040.5000041
TCGGTAC501.0822987E-940.50000436
CGGTCTA3900.040.38461731
GCTACGA2350.040.21276510
TCGATAG850.039.7058831
ATAGGGC9150.039.5901644
AATGCGT403.4599907E-739.37530
Produced by FastQC (version 0.11.5)