##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546348_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2049608
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1982910878568	31.0	31.0	33.0	30.0	34.0
2	31.531427960858856	31.0	31.0	34.0	30.0	34.0
3	31.601780925913637	31.0	31.0	34.0	30.0	34.0
4	35.452190370061004	37.0	35.0	37.0	33.0	37.0
5	35.37800691644451	37.0	35.0	37.0	33.0	37.0
6	35.41059168387321	37.0	35.0	37.0	33.0	37.0
7	35.8167815504233	37.0	35.0	37.0	35.0	37.0
8	35.72543188746336	37.0	35.0	37.0	35.0	37.0
9	37.49565429096686	39.0	37.0	39.0	35.0	39.0
10	36.99902469155077	39.0	37.0	39.0	33.0	39.0
11	36.69032078329124	39.0	35.0	39.0	32.0	39.0
12	35.947649501758384	37.0	35.0	39.0	32.0	39.0
13	35.61684429412844	37.0	35.0	39.0	31.0	39.0
14	36.52464080936452	38.0	35.0	41.0	31.0	41.0
15	36.780378979785404	38.0	35.0	41.0	31.0	41.0
16	36.872692241638404	38.0	35.0	41.0	32.0	41.0
17	36.80828577952467	38.0	35.0	41.0	32.0	41.0
18	36.72631107997236	38.0	35.0	40.0	31.0	41.0
19	36.61568358437321	38.0	35.0	40.0	31.0	41.0
20	36.484338468624244	38.0	35.0	40.0	31.0	41.0
21	36.31312085042603	38.0	35.0	40.0	31.0	41.0
22	36.196685902865326	38.0	35.0	40.0	30.0	41.0
23	36.138183496551534	37.0	35.0	40.0	30.0	41.0
24	36.13749995121018	37.0	35.0	40.0	31.0	41.0
25	36.09045241821851	37.0	35.0	40.0	30.0	41.0
26	35.97265867424405	37.0	35.0	40.0	30.0	41.0
27	35.85122764938466	37.0	34.0	40.0	30.0	41.0
28	35.80123321142384	37.0	34.0	40.0	30.0	41.0
29	35.71967420111553	37.0	34.0	40.0	30.0	41.0
30	35.56031202064005	37.0	34.0	40.0	29.0	41.0
31	35.34856762854165	37.0	34.0	40.0	29.0	41.0
32	35.14862549326505	36.0	34.0	40.0	27.0	41.0
33	34.964963056350285	36.0	34.0	40.0	27.0	41.0
34	34.742355611414474	36.0	34.0	40.0	25.0	41.0
35	34.56379414990574	36.0	33.0	40.0	25.0	41.0
36	34.35153746472496	36.0	33.0	40.0	24.0	41.0
37	34.17972753814388	36.0	33.0	40.0	23.0	41.0
38	34.081402394994555	35.0	33.0	40.0	23.0	41.0
39	34.05251687151885	36.0	33.0	40.0	23.0	41.0
40	33.8247177021167	35.0	33.0	40.0	23.0	41.0
41	33.737124855094244	35.0	33.0	40.0	23.0	41.0
42	33.604122349249224	35.0	33.0	40.0	22.0	41.0
43	33.5505086826359	35.0	33.0	40.0	23.0	41.0
44	33.368384588662806	35.0	32.0	39.0	22.0	41.0
45	33.32820666195682	35.0	32.0	39.0	22.0	41.0
46	33.234610227907	35.0	32.0	39.0	22.0	41.0
47	33.15775894707671	35.0	32.0	39.0	22.0	41.0
48	33.064082985624566	35.0	32.0	39.0	21.0	41.0
49	33.0535863443156	35.0	32.0	39.0	21.0	41.0
50	32.908961616074876	35.0	32.0	39.0	20.0	40.0
51	32.70483380236611	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	5.0
11	7.0
12	9.0
13	27.0
14	34.0
15	105.0
16	237.0
17	579.0
18	1273.0
19	2514.0
20	4343.0
21	6853.0
22	10486.0
23	15063.0
24	20924.0
25	29319.0
26	38188.0
27	44195.0
28	48421.0
29	53474.0
30	61905.0
31	75113.0
32	92705.0
33	120172.0
34	202979.0
35	271641.0
36	157101.0
37	194783.0
38	261878.0
39	335219.0
40	55.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.445216841464315	23.675063719501484	27.049318698990245	13.830400740043949
2	33.48147548214098	24.425109581929814	29.030965921288367	13.062449014640848
3	29.885714731792618	24.42676843572039	31.60506789590985	14.08244893657714
4	26.180469631266078	27.358450981846282	30.726753603615908	15.734325783271727
5	22.65750328843369	32.07989039855426	30.239831226263753	15.022775086748293
6	20.947273820164636	40.98330022131061	27.314198617491737	10.755227341033018
7	86.15344983040659	4.572679263546981	7.336671207372336	1.9371996986740878
8	87.67393569892388	3.4082614821956203	6.88243800765805	2.035364811222439
9	83.59403359081347	4.661379151525559	8.648775765902553	3.095811491758424
10	50.60372520013583	21.50264831128684	14.307906682643706	13.585719805933621
11	43.557109457027885	20.82266462660177	20.168539545122773	15.451686371247575
12	37.53825121681805	20.63731211041331	26.182567593412987	15.641869079355661
13	24.70521192345073	32.42981096873158	27.036974875195646	15.828002232622044
14	19.412834063879533	36.03474420474549	28.376060202731452	16.176361528643525
15	17.41445193422352	21.820367601999994	44.0429584583979	16.72222200537859
16	19.575694474260445	17.84258258164488	41.440899918423426	21.140823025671253
17	20.648582558225765	18.0609658041928	28.611519861358854	32.678931776222576
18	23.8207013243508	22.001914512433597	32.70298515618597	21.47439900702964
19	29.493590969590283	23.624419889071472	25.663931834770352	21.21805730656789
20	31.23075241704755	22.476444276173783	23.91154796429366	22.381255342485
21	26.00380170256947	25.194281052767163	27.45032220795391	21.35159503670946
22	24.53957049347973	22.857297590563658	25.45803880546914	27.145093110487466
23	22.76615821171658	27.47618081115999	24.56996654970121	25.18769442742222
24	22.982736211021816	23.475806105362587	33.72313144757437	19.818326236041234
25	22.336905398495713	22.268453284725666	32.43244561886956	22.962195697909063
26	20.133166927529555	28.836489709251723	26.7499443796082	24.280398983610525
27	20.845839789852498	27.91255693771687	28.446122380474705	22.795480891955926
28	19.104336048649305	25.455892053504865	34.92194605017154	20.51782584767429
29	20.214255603998424	23.706581941522476	33.70263972427898	22.376522730200115
30	22.49469166787015	26.050054449436182	30.56301497652234	20.892238906171325
31	30.2430025643928	24.083239331618532	24.102852838201255	21.57090526578741
32	29.75017661913888	24.719263390853275	25.61387348214878	19.916686507859062
33	29.215976908755238	24.674620707959765	25.179351368651957	20.93005101463304
34	22.752009164679297	26.381922787186625	27.926852354206268	22.939215693927814
35	23.347684044949084	24.246246111451555	29.27740328882401	23.12866655477535
36	30.78500864555564	24.579236615001506	24.863827619720453	19.771927119722406
37	24.469459525919103	28.425728236814063	26.39426661098122	20.71054562628561
38	24.244636047478345	30.1895777143727	22.942045503335272	22.623740734813683
39	23.689895823981953	28.078832635313677	24.839139972131257	23.392131568573113
40	25.18701136997904	24.061283913802054	25.736775032103697	25.014929684115206
41	21.38535759032947	23.375055132493628	25.92422551043907	29.315361766737837
42	23.81367559064953	25.125584989910266	24.17413476137876	26.88660465806144
43	23.712387929789504	25.445353452952958	24.97565388113239	25.866604736125154
44	22.50479116006573	27.00940862838162	27.263457207426985	23.22234300412567
45	22.17360587975847	30.00251755457629	24.303476567226515	23.520399998438727
46	24.48321825441743	28.31160885398574	26.147487714723987	21.057685176872845
47	24.29737784005527	26.02219546371794	26.446276556297594	23.234150139929195
48	24.201847377644896	24.490585516840294	27.843665715590493	23.463901389924317
49	23.365882646828076	22.79250471309636	30.206995679173776	23.63461696090179
50	21.99771858813978	27.838298835679797	26.873870515727887	23.290112060452536
51	21.287241267598485	29.632007681468846	25.23394717428894	23.84680387664373
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	673.0
1	1649.5
2	2626.0
3	10529.0
4	18432.0
5	13477.0
6	8522.0
7	8833.0
8	9144.0
9	9395.5
10	9647.0
11	9592.0
12	9537.0
13	9097.5
14	8658.0
15	8325.5
16	7993.0
17	7409.5
18	6826.0
19	6792.5
20	6759.0
21	6763.5
22	6768.0
23	6369.0
24	5970.0
25	6186.5
26	8092.5
27	9782.0
28	10740.5
29	11699.0
30	15706.0
31	19713.0
32	23131.5
33	26550.0
34	30469.0
35	34388.0
36	37426.0
37	40464.0
38	49798.0
39	59132.0
40	80738.0
41	102344.0
42	127616.0
43	152888.0
44	156695.0
45	160502.0
46	163232.5
47	165963.0
48	161347.0
49	156731.0
50	155885.0
51	155039.0
52	150692.0
53	146345.0
54	133195.0
55	120045.0
56	116860.0
57	113675.0
58	110422.0
59	107169.0
60	98704.0
61	90239.0
62	84895.0
63	79551.0
64	71028.0
65	62505.0
66	54171.0
67	45837.0
68	38544.0
69	31251.0
70	25430.0
71	19609.0
72	16702.5
73	13796.0
74	10767.0
75	5944.5
76	4151.0
77	3260.5
78	2370.0
79	1708.0
80	1046.0
81	810.0
82	574.0
83	389.5
84	205.0
85	201.0
86	197.0
87	138.0
88	79.0
89	66.0
90	53.0
91	32.0
92	11.0
93	8.5
94	6.0
95	4.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2049608.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.963868568164248
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.67624858538561	15.299337747457997
2	6.8349074812535076	2.5923257430010813
3	2.3154464224990385	1.3172946489869362
4	1.18705448147676	0.9004458067990257
5	0.7618202445198239	0.7223529494820344
6	0.5794583680356753	0.6593263399290896
7	0.501482462735852	0.6657033258793416
8	0.3712965362414887	0.563297497047858
9	0.32924857118797585	0.561944396723818
>10	3.8269475659069094	16.829263125058443
>50	1.2522242225711724	17.25127439430359
>100	1.3543022370496312	40.360780386565274
>500	0.0064613656327899335	0.8026858265244902
>1k	0.0028430008784275707	0.9965812365343139
>5k	2.5845462531159735E-4	0.4773865757066944
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9740	0.4752128211833678	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4440	0.21662678912260294	No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	2086	0.10177555903372743	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05381516855906105	0.0
2	0.0	0.0	0.0	0.16827608010897693	0.0
3	0.0	0.0	0.0	0.24160717561602024	0.0
4	0.0	0.0	0.0	0.35855636785180384	0.0
5	0.0	0.0	0.0	0.5964067275303375	0.0
6	0.0	0.0	0.0	0.8454787452039609	0.0
7	0.0	0.0	0.0	0.9980932939371822	0.0
8	0.0	0.0	0.0	1.4407633069347894	0.0
9	0.0	0.0	0.0	1.6451926417149034	0.0
10	0.0	0.0	0.0	2.0012119390634697	0.0
11	0.0	0.0	0.0	2.263603576879091	0.0
12	0.0	0.0	0.0	2.5138465501695935	0.0
13	0.0	0.0	0.0	2.6136705165085226	0.0
14	0.0	0.0	0.0	2.651775363874458	0.0
15	0.0	0.0	0.0	2.709054609466786	0.0
16	0.0	0.0	0.0	2.846495524997951	0.0
17	0.0	0.0	0.0	2.999354022818022	0.0
18	0.0	0.0	0.0	3.239546293730313	0.0
19	0.0	0.0	0.0	3.3369307692007446	0.0
20	0.0	0.0	0.0	3.4503670945858915	0.0
21	0.0	0.0	0.0	3.579562530981534	0.0
22	0.0	0.0	0.0	3.702610450388562	0.0
23	0.0	0.0	0.0	3.8527855082532856	0.0
24	0.0	0.0	0.0	3.9558783923560017	0.0
25	0.0	0.0	0.0	4.0431633756308525	0.0
26	0.0	0.0	0.0	4.129911670914633	0.0
27	0.0	0.0	0.0	4.220611941405381	0.0
28	0.0	0.0	0.0	4.315264187103095	0.0
29	0.0	0.0	0.0	4.412599872756156	0.0
30	0.0	0.0	0.0	4.544381169472406	0.0
31	0.0	0.0	0.0	4.652889723303188	0.0
32	0.0	0.0	0.0	4.758958786265471	0.0
33	0.0	0.0	0.0	4.868491926261021	0.0
34	0.0	0.0	0.0	4.986953602835274	0.0
35	0.0	0.0	0.0	5.1252239452617285	0.0
36	0.0	0.0	0.0	5.246613010878177	0.0
37	0.0	0.0	0.0	5.36497710781769	0.0
38	0.0	0.0	0.0	5.490513307910586	0.0
39	0.0	0.0	0.0	5.627759064172271	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCAAC	25	3.8918784E-5	45.000004	15
CATACGT	25	3.8918784E-5	45.000004	10
ACCGGAT	20	7.0345815E-4	45.0	6
TCACCGT	20	7.0345815E-4	45.0	16
CGACGTA	20	7.0345815E-4	45.0	11
ACGTATC	20	7.0345815E-4	45.0	34
CGTAGCA	30	2.1663382E-6	44.999996	17
TAGCCGT	135	0.0	43.333336	44
CGTTTTT	8095	0.0	42.970966	1
TGCAACG	110	0.0	42.954548	1
CCGTAGG	85	0.0	42.35294	2
TACGTAG	70	0.0	41.785713	1
GCGCGAC	1120	0.0	40.580357	9
TCGTTAG	100	0.0	40.500004	1
TCGGTAC	50	1.0822987E-9	40.500004	36
CGGTCTA	390	0.0	40.384617	31
GCTACGA	235	0.0	40.212765	10
TCGATAG	85	0.0	39.705883	1
ATAGGGC	915	0.0	39.590164	4
AATGCGT	40	3.4599907E-7	39.375	30
>>END_MODULE