##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546347_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1794290
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.112654587608468	31.0	31.0	33.0	30.0	34.0
2	31.44566430175724	31.0	31.0	34.0	30.0	34.0
3	31.542397271344097	31.0	31.0	34.0	30.0	34.0
4	35.399963773971876	37.0	35.0	37.0	33.0	37.0
5	35.30142786283154	37.0	35.0	37.0	33.0	37.0
6	35.338615831331616	37.0	35.0	37.0	33.0	37.0
7	35.755485456643015	37.0	35.0	37.0	35.0	37.0
8	35.6589001777862	37.0	35.0	37.0	35.0	37.0
9	37.41563738303173	39.0	37.0	39.0	35.0	39.0
10	36.90103550708079	39.0	37.0	39.0	32.0	39.0
11	36.60794074536446	39.0	35.0	39.0	32.0	39.0
12	35.84730060358136	37.0	35.0	39.0	31.0	39.0
13	35.49108951172887	37.0	35.0	39.0	30.0	39.0
14	36.41533810030708	38.0	35.0	40.0	31.0	41.0
15	36.69603687252339	38.0	35.0	41.0	31.0	41.0
16	36.78293977004832	38.0	35.0	41.0	32.0	41.0
17	36.70616344069242	38.0	35.0	40.0	31.0	41.0
18	36.6369739562724	38.0	35.0	40.0	31.0	41.0
19	36.52304644176805	38.0	35.0	40.0	31.0	41.0
20	36.36684036582715	38.0	35.0	40.0	31.0	41.0
21	36.21392194126925	38.0	35.0	40.0	30.0	41.0
22	36.08711858172313	37.0	35.0	40.0	30.0	41.0
23	36.03112428871587	37.0	34.0	40.0	30.0	41.0
24	36.01151987694297	37.0	34.0	40.0	30.0	41.0
25	35.936822364277795	37.0	34.0	40.0	30.0	41.0
26	35.808888752654255	37.0	34.0	40.0	30.0	41.0
27	35.68519358632105	37.0	34.0	40.0	30.0	41.0
28	35.646422819053775	37.0	34.0	40.0	29.0	41.0
29	35.568750870817986	37.0	34.0	40.0	29.0	41.0
30	35.414955219056004	36.0	34.0	40.0	29.0	41.0
31	35.20020286575749	36.0	34.0	40.0	28.0	41.0
32	35.03684967312976	36.0	34.0	40.0	27.0	41.0
33	34.823099387501465	36.0	34.0	40.0	26.0	41.0
34	34.61018397249051	36.0	34.0	40.0	25.0	41.0
35	34.43475023546918	36.0	33.0	40.0	24.0	41.0
36	34.22441244169003	36.0	33.0	40.0	23.0	41.0
37	34.041688913163426	36.0	33.0	40.0	23.0	41.0
38	33.96259356068417	35.0	33.0	40.0	23.0	41.0
39	33.933554219217626	35.0	33.0	40.0	23.0	41.0
40	33.66989561330666	35.0	33.0	40.0	22.0	41.0
41	33.58152249636346	35.0	33.0	40.0	22.0	41.0
42	33.45424206789315	35.0	32.0	40.0	22.0	41.0
43	33.393459251291596	35.0	32.0	40.0	22.0	41.0
44	33.22802501267911	35.0	32.0	39.0	21.0	41.0
45	33.18919238250227	35.0	32.0	39.0	21.0	41.0
46	33.08568403100948	35.0	32.0	39.0	21.0	41.0
47	33.0272737405882	35.0	32.0	39.0	20.0	41.0
48	32.930891327488865	35.0	32.0	39.0	20.0	41.0
49	32.957165787024394	35.0	32.0	39.0	20.0	41.0
50	32.83249251793188	35.0	32.0	39.0	20.0	40.0
51	32.61033110589704	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	5.0
13	6.0
14	28.0
15	102.0
16	231.0
17	626.0
18	1382.0
19	2649.0
20	4514.0
21	7055.0
22	10303.0
23	14509.0
24	20173.0
25	27717.0
26	35125.0
27	40594.0
28	44186.0
29	48820.0
30	56183.0
31	66934.0
32	82462.0
33	104809.0
34	176633.0
35	230701.0
36	133171.0
37	166020.0
38	225906.0
39	293391.0
40	49.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.97956294690379	23.83934592512916	28.89427015699803	14.28682097096902
2	31.073628008850296	25.57908699262661	30.402666235669816	12.944618762853274
3	29.315439533185828	24.792759252963567	31.915911028874934	13.975890184975674
4	27.066862101444023	27.08714867719265	30.77389942539946	15.072089795963864
5	22.88526380908326	32.60576606903009	29.9726911480307	14.536278973855953
6	20.814138182790963	41.65157248828228	27.18601786779172	10.348271461135045
7	86.81093914584599	4.573619648997654	6.940628326524697	1.6748128786316592
8	88.18970177619002	3.4051351788172477	6.4379225208856985	1.9672405241070285
9	84.12631180021067	4.677226089428131	8.198897614098055	2.997564496263146
10	49.43966694347068	23.84057203685023	13.673988039837484	13.045772979841608
11	42.66099682882923	22.008370999113854	19.93111481421621	15.399517357840706
12	39.19717548445346	20.31304861532974	25.30454943180868	15.185226468408116
13	24.5028395632813	33.20282674484058	25.093268089327815	17.20106560255031
14	18.517909590980278	36.726281704741154	27.66152628616333	17.094282418115245
15	17.14862146029906	22.28870472443139	43.30949846457373	17.25317535069582
16	18.768482240886367	17.454982193513867	41.91791739350941	21.858618172090353
17	18.511890497076838	18.492495638943538	29.36771647838421	33.62789738559542
18	23.12011993601926	22.930852872166707	31.68306126657341	22.265965925240625
19	29.24616422094533	24.62093641496079	24.673603486615875	21.459295877478
20	31.7250277268446	22.904380005461768	23.516990007189474	21.853602260504154
21	25.03697841486047	26.3747220348996	26.61665617040724	21.971643379832692
22	24.373874903165042	23.700126512436672	24.88527495555345	27.040723628844837
23	22.602645057376456	28.324629797858762	23.847036989561328	25.22568815520345
24	23.816384196534564	23.164259957977805	33.180979663265134	19.838376182222493
25	21.36505247200843	23.280294712671864	31.20170095135123	24.15295186396848
26	20.20554091033222	29.359635287495333	26.72862246348138	23.70620133869107
27	20.074291223826695	28.762964738141548	28.20190716104977	22.960836876981983
28	18.22091189272637	27.00533358598666	34.89151697886072	19.88223754242625
29	20.12818440720285	24.527027403596964	33.205111771229845	22.13967641797034
30	22.09364149607923	26.50385389206873	30.115756092939268	21.286748518912773
31	28.90179402437733	25.187344297744513	23.93013392483935	21.980727753038806
32	29.656911647504025	25.29290137045851	25.117065803186776	19.933121178850687
33	27.666765127153358	26.015248371222043	24.94875410329434	21.369232398330258
34	21.342926728678197	26.737595371985577	28.423331791404955	23.49614610793127
35	22.03216871297282	24.881875282145028	28.757893094204395	24.32806291067776
36	28.655289836091157	25.871068779294315	24.60856383304817	20.86507755156636
37	23.050844623778765	29.448361190220083	26.36725389987126	21.13354028612989
38	22.717732362104233	30.476400135986935	22.653974552608553	24.15189294930028
39	22.117160548183403	28.76809211442966	24.712616132286307	24.402131205100623
40	23.618701547687387	24.87936732635193	25.446778391452884	26.055152734507796
41	19.415311906102133	23.750508557702492	26.716751472727374	30.117428063467997
42	22.74682464930418	25.90985849556092	23.08551014607449	28.257806709060407
43	22.540001894899934	26.305446722659102	24.89469372286531	26.25985765957565
44	21.62643719800032	27.677019879729585	26.27657736486298	24.41996555740711
45	20.862458131071342	31.276103639879842	23.88259422947238	23.978843999576434
46	23.10273144252044	29.201745537232	26.23756471919255	21.457958301055015
47	23.789744132776754	26.433575397510996	26.347357450579338	23.429323019132916
48	23.077707616940405	23.805572120448755	28.34380172658823	24.772918536022605
49	21.031605816228147	24.008159216180218	30.08610648222974	24.874128485361897
50	20.52327104314241	27.66498169192271	27.96827714583484	23.843470119100036
51	20.818373841463757	29.630717442553877	24.673492021913958	24.877416694068405
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	723.0
1	1296.0
2	1869.0
3	8801.0
4	15733.0
5	11631.0
6	7529.0
7	7724.0
8	7919.0
9	8042.0
10	8165.0
11	8184.5
12	8204.0
13	7957.5
14	7711.0
15	7430.5
16	7150.0
17	6696.5
18	6243.0
19	6011.5
20	5780.0
21	5917.0
22	6054.0
23	6113.0
24	6172.0
25	6847.5
26	9076.0
27	10629.0
28	11881.0
29	13133.0
30	17040.5
31	20948.0
32	23775.5
33	26603.0
34	30975.0
35	35347.0
36	38146.0
37	40945.0
38	48049.0
39	55153.0
40	75406.0
41	95659.0
42	113605.0
43	131551.0
44	136338.5
45	141126.0
46	137002.5
47	132879.0
48	136736.0
49	140593.0
50	136752.5
51	132912.0
52	125171.0
53	117430.0
54	110446.5
55	103463.0
56	100083.5
57	96704.0
58	95300.5
59	93897.0
60	89933.0
61	85969.0
62	77463.0
63	68957.0
64	60947.5
65	52938.0
66	44226.5
67	35515.0
68	29254.5
69	22994.0
70	19947.5
71	16901.0
72	14228.5
73	11556.0
74	8996.0
75	5198.5
76	3961.0
77	2836.5
78	1712.0
79	1346.0
80	980.0
81	637.5
82	295.0
83	229.5
84	164.0
85	117.0
86	70.0
87	51.5
88	33.0
89	34.0
90	35.0
91	25.0
92	15.0
93	10.0
94	5.0
95	4.0
96	3.0
97	3.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1794290.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.369926451699232
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.52735261033298	15.791788238638993
2	6.640018216426263	2.5723332898023967
3	2.239432183525713	1.3013291006538386
4	1.1131263888145158	0.8624470513113093
5	0.7130638339816124	0.6905997009795252
6	0.5270184830036355	0.6124985552679912
7	0.41151508853736213	0.5579711899113242
8	0.34377227552956235	0.5327074954512725
9	0.28406002854785484	0.4951999674755579
>10	3.5207964717480347	16.092025540431802
>50	1.3254841149170407	18.567322783132738
>100	1.343098033445575	39.380099559658916
>500	0.007796956977603714	0.9883844289634726
>1k	0.003176538027912624	1.1022695844456247
>5k	2.8877618435569307E-4	0.4530235138752215
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8099	0.45137631040690185	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4633	0.25820798198730416	No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	2209	0.12311276326569284	No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	2064	0.11503157237681756	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05857470085660623	0.0
2	0.0	0.0	0.0	0.17661582018514288	0.0
3	0.0	0.0	0.0	0.24628125888234342	0.0
4	0.0	0.0	0.0	0.34860585524079163	0.0
5	0.0	0.0	0.0	0.5435576188910377	0.0
6	0.0	0.0	0.0	0.7608023229243879	0.0
7	0.0	0.0	0.0	0.9016379737946486	0.0
8	0.0	0.0	0.0	1.3461592050337459	0.0
9	0.0	0.0	0.0	1.5377670276265263	0.0
10	0.0	0.0	0.0	1.8369382875677844	0.0
11	0.0	0.0	0.0	2.0779249731091407	0.0
12	0.0	0.0	0.0	2.310328876603002	0.0
13	0.0	0.0	0.0	2.4004480881017005	0.0
14	0.0	0.0	0.0	2.435448004503174	0.0
15	0.0	0.0	0.0	2.489954243739864	0.0
16	0.0	0.0	0.0	2.624380674249982	0.0
17	0.0	0.0	0.0	2.7822146921623596	0.0
18	0.0	0.0	0.0	3.0427634328898896	0.0
19	0.0	0.0	0.0	3.1370068383594627	0.0
20	0.0	0.0	0.0	3.2521498754381954	0.0
21	0.0	0.0	0.0	3.375541300458677	0.0
22	0.0	0.0	0.0	3.493136560979552	0.0
23	0.0	0.0	0.0	3.6390995881379262	0.0
24	0.0	0.0	0.0	3.746941687241193	0.0
25	0.0	0.0	0.0	3.832212184206566	0.0
26	0.0	0.0	0.0	3.911853713725206	0.0
27	0.0	0.0	0.0	3.9961210283733397	0.0
28	0.0	0.0	0.0	4.091813474967815	0.0
29	0.0	0.0	0.0	4.1894008214948535	0.0
30	0.0	0.0	0.0	4.3120677259528835	0.0
31	0.0	0.0	0.0	4.430443239387167	0.0
32	0.0	0.0	0.0	4.537114959120321	0.0
33	0.0	0.0	0.0	4.655323275501731	0.0
34	0.0	0.0	0.0	4.7732529301283515	0.0
35	0.0	0.0	0.0	4.912639539873711	0.0
36	0.0	0.0	0.0	5.036142429596108	0.0
37	0.0	0.0	0.0	5.154462210679433	0.0
38	0.0	0.0	0.0	5.280250126791098	0.0
39	0.0	0.0	0.0	5.409047589854483	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	20	7.0343463E-4	45.0	10
CCGAACA	20	7.0343463E-4	45.0	29
ACGTAAG	20	7.0343463E-4	45.0	1
CGGTCGA	25	3.891685E-5	45.0	21
GACGATA	20	7.0343463E-4	45.0	33
ATCGCTA	20	7.0343463E-4	45.0	11
TAATCCG	20	7.0343463E-4	45.0	36
CGGTCTA	210	0.0	43.92857	31
CGTTTTT	6930	0.0	43.603897	1
TATAGCG	115	0.0	43.04348	1
CTATACG	95	0.0	42.63158	1
CAATTCG	115	0.0	41.08696	14
ACGGGAC	395	0.0	41.01266	5
CGTATGA	55	6.184564E-11	40.909092	15
GACGTAG	110	0.0	40.909092	1
ACGGTCT	220	0.0	40.909092	30
CATACGA	420	0.0	40.714287	18
ATACCCG	50	1.0822987E-9	40.5	22
AACGGGA	375	0.0	40.2	4
CGCATCG	45	1.929402E-8	40.0	21
>>END_MODULE