FastQCFastQC Report
Thu 26 May 2016
SRR1546346_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546346_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2172190
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121280.5583305327802817No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC97780.45014478475639796No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGAG900.045.0000041
TAATACG750.045.01
CGTATAT253.891957E-545.014
GCGCCTA207.034675E-445.027
TACGGTG207.034675E-445.029
CGCGAAA253.891957E-545.037
CGTAGTC253.891957E-545.013
TGCGAAC351.2125929E-745.010
TATTCGA351.2125929E-745.039
TCGCTAA502.1827873E-1145.015
TCGTCAG253.891957E-545.030
ATGCGTA253.891957E-545.034
CGTTTCA406.8193913E-945.030
ACGACGT406.8193913E-945.021
AGTCGTT207.034675E-445.018
TAACTAC253.891957E-545.010
CGAAGTA351.2125929E-745.043
TACAACG207.034675E-445.014
CGGACGT207.034675E-445.033
CCGCTTA302.1663982E-644.99999641