##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546345_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2231303
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.12911245133449	31.0	31.0	33.0	30.0	34.0
2	31.451150740172896	31.0	31.0	34.0	30.0	34.0
3	31.532214584930866	31.0	31.0	34.0	30.0	34.0
4	35.39083441379319	37.0	35.0	37.0	33.0	37.0
5	35.30087800715546	37.0	35.0	37.0	33.0	37.0
6	35.34423652905948	37.0	35.0	37.0	33.0	37.0
7	35.77960635556892	37.0	35.0	37.0	35.0	37.0
8	35.67655849519317	37.0	35.0	37.0	35.0	37.0
9	37.43187680023735	39.0	37.0	39.0	35.0	39.0
10	36.9042926935517	39.0	37.0	39.0	32.0	39.0
11	36.59317806680671	39.0	35.0	39.0	32.0	39.0
12	35.92564568774389	37.0	35.0	39.0	31.0	39.0
13	35.633958722773194	37.0	35.0	39.0	30.0	39.0
14	36.44455369799619	38.0	35.0	41.0	30.0	41.0
15	36.73030377317648	38.0	35.0	41.0	31.0	41.0
16	36.81988685534865	38.0	35.0	41.0	31.0	41.0
17	36.7552654211463	38.0	35.0	41.0	31.0	41.0
18	36.7011997025953	38.0	35.0	40.0	31.0	41.0
19	36.600718055772795	38.0	35.0	40.0	31.0	41.0
20	36.4353810307251	38.0	35.0	40.0	31.0	41.0
21	36.2911572296546	38.0	35.0	40.0	30.0	41.0
22	36.22214105390438	38.0	35.0	40.0	30.0	41.0
23	36.141238101683186	38.0	35.0	40.0	30.0	41.0
24	36.142780249925714	38.0	35.0	40.0	30.0	41.0
25	36.08276150751377	38.0	35.0	40.0	30.0	41.0
26	35.9669291889089	38.0	34.0	40.0	30.0	41.0
27	35.850522766293956	37.0	34.0	40.0	30.0	41.0
28	35.78347091363208	37.0	34.0	40.0	30.0	41.0
29	35.664838885619744	37.0	34.0	40.0	29.0	41.0
30	35.485086964881056	37.0	34.0	40.0	29.0	41.0
31	35.25423440922187	37.0	34.0	40.0	27.0	41.0
32	35.09064793082786	37.0	34.0	40.0	27.0	41.0
33	34.8948986309793	37.0	34.0	40.0	26.0	41.0
34	34.664828577741346	37.0	33.0	40.0	24.0	41.0
35	34.4649570228696	36.0	33.0	40.0	24.0	41.0
36	34.25023629690813	36.0	33.0	40.0	23.0	41.0
37	34.06890413359369	36.0	33.0	40.0	23.0	41.0
38	33.92678045070526	36.0	33.0	40.0	23.0	41.0
39	33.868447270496205	36.0	33.0	40.0	23.0	41.0
40	33.61448400329314	36.0	32.0	40.0	21.0	41.0
41	33.49327142033153	35.0	32.0	40.0	21.0	41.0
42	33.39170296459064	35.0	32.0	40.0	21.0	41.0
43	33.3393873445247	35.0	32.0	40.0	21.0	41.0
44	33.12174007743457	35.0	32.0	39.0	20.0	41.0
45	33.091413851009925	35.0	32.0	39.0	20.0	41.0
46	33.03354138814854	35.0	32.0	39.0	20.0	41.0
47	32.94267206201937	35.0	31.0	39.0	20.0	41.0
48	32.86287967165374	35.0	31.0	39.0	20.0	41.0
49	32.86228315921235	35.0	31.0	39.0	20.0	41.0
50	32.71177603400345	35.0	31.0	39.0	20.0	40.0
51	32.49666853851763	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	6.0
11	7.0
12	7.0
13	18.0
14	44.0
15	112.0
16	320.0
17	848.0
18	1735.0
19	3306.0
20	5677.0
21	8922.0
22	13086.0
23	18430.0
24	25441.0
25	34589.0
26	44129.0
27	50779.0
28	54980.0
29	61054.0
30	70103.0
31	83692.0
32	101502.0
33	130850.0
34	208911.0
35	264926.0
36	173655.0
37	219107.0
38	290392.0
39	364613.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.997486670344635	22.8857757104257	27.758757999249767	13.357979619979895
2	32.97364813295191	25.015831556718204	29.53184753482606	12.47867277550382
3	30.106758248431518	25.257932248556113	31.52601865367456	13.10929084933781
4	26.24013860959269	27.802409623435274	30.390404171912106	15.567047595059927
5	23.362761579220752	32.26348012797903	29.452432054275015	14.921326238525204
6	20.807841875352654	41.72257196803841	27.165203470797106	10.304382685811833
7	87.23575417592322	3.895526515224512	7.217083470958449	1.6516358378938227
8	88.38001831217007	3.02195622916296	6.52246691731244	2.075558541354536
9	84.49278291652904	4.095185638167474	8.421715921145626	2.9903155241578574
10	49.34444134212162	22.911186871527534	13.889104258812004	13.855267527538842
11	40.34230223326908	23.162788738239495	20.28895224001402	16.205956788477405
12	35.75005277185573	20.864311122245613	26.984053712113504	16.401582393785155
13	25.59078708718628	29.847089346449135	27.77542987214197	16.786693694222613
14	20.538895882809282	32.80858762794654	29.77829546233748	16.8742210269067
15	18.717538586198287	22.18981465090129	41.54756211953285	17.545084643367577
16	21.539432340654766	18.373120997013853	38.3491619022607	21.738284760070687
17	22.013818831418234	18.36958046486739	29.03599376687075	30.58060693684363
18	25.367016492157273	21.620147510221607	31.992427742892833	21.02040825472829
19	28.842922722731963	24.44020377331093	25.304989954300243	21.41188354965686
20	29.825980604158197	23.85502999816699	24.65147046367078	21.667518934004033
21	25.46189379030997	25.44199510330959	28.800929322463155	20.29518178391729
22	24.700365660782065	21.79538144304023	27.188373788768267	26.31587910740944
23	22.706149725070958	27.286433084166518	25.262369117954847	24.74504807280768
24	22.673523049088356	25.105778999983418	32.14722518635972	20.073472764568507
25	23.213028441229184	23.146206499072516	30.446470067041542	23.194294992656758
26	20.633325012335842	26.981543967807152	26.687141997299335	25.69798902255767
27	21.485428021205546	26.09596276256519	29.022593524949325	23.39601569127994
28	19.6205983678595	25.907911207039113	32.84856426939775	21.622926155703638
29	21.087050929434508	25.299253395885724	31.92529208269787	21.688403591981906
30	24.281462445934057	25.051729863671586	29.97365216646955	20.693155523924812
31	29.209031673421315	23.5167523191606	25.145755641434626	22.128460365983464
32	29.13113996619912	24.688713276502565	25.877346106736738	20.30280065056158
33	28.18263588584787	23.73209734401827	26.044961172911073	22.04030559722279
34	21.988318036591174	26.343665562229784	28.325960212485708	23.342056188693334
35	22.950580893764762	24.28043165809395	29.78479390741643	22.98419354072486
36	30.437865229419756	24.305394650569646	24.53306431264602	20.723675807364575
37	24.29315964707617	28.681357933010442	25.561028690410943	21.46445372950245
38	24.494252909622762	29.00412897755258	23.168883831554925	23.332734281269733
39	23.424115864138578	26.694895314531465	24.170451077240518	25.71053774408944
40	25.749125062799628	23.49308901570069	25.64770450270537	25.110081418794312
41	22.084674291210113	22.866907811265435	26.41012896948554	28.638288928038907
42	24.289081312578347	24.536873745968162	24.896125716677656	26.277919224775836
43	23.168211578615725	24.976303083893132	26.28558290828274	25.5699024292084
44	22.883893402195937	25.733663245197985	27.150772441035574	24.231670911570504
45	21.662140910490418	28.447145008992504	25.569947246071017	24.32076683444606
46	24.629241299814502	26.224497524540592	27.316863733881053	21.82939744176385
47	23.87353936242635	26.17246514704637	26.7009904078469	23.25300508268039
48	22.864801418722603	25.798199527361366	27.358812317287253	23.978186736628775
49	23.23579540743682	23.424474399039486	29.524004583868706	23.815725609654987
50	22.860902351675232	26.453422058770144	27.5857200926992	23.099955496855426
51	22.725420975994744	28.48882469122302	24.876944099479093	23.90881023330314
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	622.0
1	1504.0
2	2386.0
3	11161.5
4	19937.0
5	14539.0
6	9141.0
7	9099.5
8	9058.0
9	9363.5
10	9669.0
11	9548.0
12	9427.0
13	9317.0
14	9207.0
15	8637.5
16	8068.0
17	7974.5
18	7881.0
19	7256.0
20	6631.0
21	6253.0
22	5875.0
23	6172.5
24	6470.0
25	7600.5
26	10234.0
27	11737.0
28	13911.0
29	16085.0
30	18946.5
31	21808.0
32	26309.5
33	30811.0
34	35487.5
35	40164.0
36	44590.5
37	49017.0
38	58621.0
39	68225.0
40	86776.5
41	105328.0
42	123885.5
43	142443.0
44	146902.5
45	151362.0
46	162493.5
47	173625.0
48	174133.0
49	174641.0
50	175304.5
51	175968.0
52	170593.5
53	165219.0
54	151582.5
55	137946.0
56	133860.0
57	129774.0
58	125863.0
59	121952.0
60	113655.5
61	105359.0
62	96701.5
63	88044.0
64	77752.0
65	67460.0
66	58397.0
67	49334.0
68	42229.5
69	35125.0
70	28730.0
71	22335.0
72	18202.0
73	14069.0
74	12011.5
75	7733.0
76	5512.0
77	3914.5
78	2317.0
79	1820.5
80	1324.0
81	994.0
82	664.0
83	471.0
84	278.0
85	230.5
86	183.0
87	129.0
88	75.0
89	58.0
90	41.0
91	26.5
92	12.0
93	7.0
94	2.0
95	2.5
96	3.0
97	3.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2231303.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.38451946169963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.70043646664627	16.450396177220053
2	6.8157896027493825	2.7787319160818957
3	2.3756828076530203	1.4528145728228463
4	1.1866034992008243	0.9675336849112035
5	0.8691750467653343	0.8858857828205822
6	0.6406371463051156	0.7835448226046606
7	0.5086786727423712	0.7258419212987883
8	0.42242320730562916	0.6888715272316139
9	0.344291714323863	0.6316399036023044
>10	3.6964416323712217	17.368754384569943
>50	1.266135509928857	18.762329818794367
>100	1.1608628017881322	35.41492669459908
>500	0.009077889327961917	1.2465492883187625
>1k	0.003542590957253431	1.3533357619840725
>5k	0.0	0.0
>10k+	2.2141193482833944E-4	0.48884374313988627
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10831	0.4854114389663797	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3698	0.1657327579445732	No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	3673	0.16461233637923672	No Hit
GTGCAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	2922	0.13095487255652863	No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	2641	0.1183613341621465	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.045040946926526784	0.0
2	0.0	0.0	0.0	0.1657327579445732	0.0
3	0.0	0.0	0.0	0.230672391871476	0.0
4	0.0	0.0	0.0	0.34768922015521875	0.0
5	0.0	0.0	0.0	0.6021145492118283	0.0
6	0.0	0.0	0.0	0.8503999680903939	0.0
7	0.0	0.0	0.0	1.022362269938238	0.0
8	0.0	0.0	0.0	1.5500808272117235	0.0
9	0.0	0.0	0.0	1.79513943198212	0.0
10	0.0	0.0	0.0	2.2359580926481075	0.0
11	0.0	0.0	0.0	2.5269091647346866	0.0
12	0.0	0.0	0.0	2.841478723418559	0.0
13	0.0	0.0	0.0	2.952624542699938	0.0
14	0.0	0.0	0.0	3.002326443338265	0.0
15	0.0	0.0	0.0	3.072420016465715	0.0
16	0.0	0.0	0.0	3.210097418414263	0.0
17	0.0	0.0	0.0	3.370676237158288	0.0
18	0.0	0.0	0.0	3.626311621505461	0.0
19	0.0	0.0	0.0	3.7332446557011756	0.0
20	0.0	0.0	0.0	3.856266943575122	0.0
21	0.0	0.0	0.0	3.987266632994264	0.0
22	0.0	0.0	0.0	4.124047697690543	0.0
23	0.0	0.0	0.0	4.2809067168376504	0.0
24	0.0	0.0	0.0	4.400164388252066	0.0
25	0.0	0.0	0.0	4.493473096213289	0.0
26	0.0	0.0	0.0	4.594579938269254	0.0
27	0.0	0.0	0.0	4.708818121070962	0.0
28	0.0	0.0	0.0	4.816871576832012	0.0
29	0.0	0.0	0.0	4.935994797658588	0.0
30	0.0	0.0	0.0	5.098097389731471	0.0
31	0.0	0.0	0.0	5.226676968569486	0.0
32	0.0	0.0	0.0	5.3548083787813665	0.0
33	0.0	0.0	0.0	5.486301053689257	0.0
34	0.0	0.0	0.0	5.629670197189713	0.0
35	0.0	0.0	0.0	5.8016773159001715	0.0
36	0.0	0.0	0.0	5.940295871963602	0.0
37	0.0	0.0	0.0	6.086891829572227	0.0
38	0.0	0.0	0.0	6.2761982572514805	0.0
39	0.0	0.0	0.0	6.641814222452083	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCCGT	20	7.0347154E-4	45.000004	14
CGTCGAC	35	1.2126111E-7	45.0	18
CTTAACG	30	2.1664255E-6	44.999996	1
CGTTTTT	8465	0.0	43.033077	1
TAATACG	85	0.0	42.352943	1
ACGGTCT	300	0.0	41.25	30
ATTTACG	105	0.0	40.714287	1
TCGATAG	100	0.0	40.5	1
TAGTACG	150	0.0	40.5	1
AGGGTAC	900	0.0	40.0	6
TATAGCG	120	0.0	39.374996	1
CGGTCTA	315	0.0	39.285713	31
AGGGCGA	2120	0.0	39.162735	6
ATACTAT	760	0.0	38.782894	45
GTGCAAG	1150	0.0	38.739132	1
TACGGGA	570	0.0	38.68421	4
TCACGAC	320	0.0	38.67188	25
ATATCGC	35	6.2509353E-6	38.571426	14
TTACGTA	35	6.2509353E-6	38.571426	30
GCGTATC	35	6.2509353E-6	38.571426	33
>>END_MODULE