FastQCFastQC Report
Thu 26 May 2016
SRR1546344_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546344_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2444859
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128030.52367028119004No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC74170.3033712782618548No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG29610.12111127880994364No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGT207.0348475E-445.00000411
CAATCGA207.0348475E-445.00000426
ACGGTCC551.8189894E-1245.00000410
TATCTCG351.2126839E-745.01
GACGATT253.8921004E-545.030
CGTTTCA253.8921004E-545.026
AGTACGT253.8921004E-545.05
ATCGCAA253.8921004E-545.016
CGTTTTT102700.042.852971
GTACGGG8550.042.3684233
CGTTGAT3300.042.27272825
TCACGAC3950.042.151925
CGTATGA700.041.78571315
TCGTTGA3350.041.64179224
ACAATCG603.6379788E-1241.24999615
TAGCCGT1900.040.26315744
GTAGGGC8950.040.2234654
CTAAGCG1400.040.178571
TATAGCG900.040.01
ATAACGG900.040.02
Produced by FastQC (version 0.11.5)