##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546344_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2444859
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.220159117560563	31.0	31.0	33.0	30.0	34.0
2	31.556094645948907	31.0	31.0	34.0	30.0	34.0
3	31.631680599985522	31.0	31.0	34.0	30.0	34.0
4	35.48239959850445	37.0	35.0	37.0	33.0	37.0
5	35.413892580308314	37.0	35.0	37.0	33.0	37.0
6	35.452886240065375	37.0	35.0	37.0	33.0	37.0
7	35.84541276204477	37.0	35.0	37.0	35.0	37.0
8	35.7515590878656	37.0	35.0	37.0	35.0	37.0
9	37.516963963975016	39.0	37.0	39.0	35.0	39.0
10	37.026194557641155	39.0	37.0	39.0	33.0	39.0
11	36.74221008246283	39.0	35.0	39.0	32.0	39.0
12	35.97545011798226	37.0	35.0	39.0	32.0	39.0
13	35.637837192247076	37.0	35.0	39.0	31.0	39.0
14	36.49054239937763	38.0	35.0	41.0	31.0	41.0
15	36.793435531456005	38.0	35.0	41.0	31.0	41.0
16	36.91642462816874	38.0	35.0	41.0	32.0	41.0
17	36.87062444091868	38.0	35.0	41.0	32.0	41.0
18	36.76614847727415	38.0	35.0	40.0	32.0	41.0
19	36.64643605214043	38.0	35.0	40.0	31.0	41.0
20	36.480455519111736	38.0	35.0	40.0	31.0	41.0
21	36.32904228832828	38.0	35.0	40.0	31.0	41.0
22	36.232815880179594	38.0	35.0	40.0	31.0	41.0
23	36.198538647832045	37.0	35.0	40.0	31.0	41.0
24	36.19332566826962	37.0	35.0	40.0	31.0	41.0
25	36.12482069518119	37.0	35.0	40.0	31.0	41.0
26	36.02999232266564	37.0	35.0	40.0	31.0	41.0
27	35.91628474280112	37.0	34.0	40.0	30.0	41.0
28	35.87126415061155	37.0	35.0	40.0	30.0	41.0
29	35.77870298450749	37.0	34.0	40.0	30.0	41.0
30	35.624362386542536	37.0	34.0	40.0	30.0	41.0
31	35.38733562958028	36.0	34.0	40.0	29.0	41.0
32	35.19514745022106	36.0	34.0	40.0	28.0	41.0
33	34.99823589008609	36.0	34.0	40.0	27.0	41.0
34	34.76102180125725	36.0	34.0	40.0	25.0	41.0
35	34.56553936239268	36.0	33.0	40.0	25.0	41.0
36	34.3525827051785	36.0	33.0	40.0	23.0	41.0
37	34.16495347993484	36.0	33.0	40.0	23.0	41.0
38	34.0706093889259	35.0	33.0	40.0	23.0	41.0
39	34.022382476862674	36.0	33.0	40.0	23.0	41.0
40	33.75895665148788	35.0	33.0	40.0	22.0	41.0
41	33.67202321279059	35.0	33.0	40.0	22.0	41.0
42	33.56715867868045	35.0	33.0	40.0	22.0	41.0
43	33.518546059302395	35.0	33.0	40.0	22.0	41.0
44	33.347435578084465	35.0	32.0	39.0	22.0	41.0
45	33.305297769728234	35.0	32.0	39.0	22.0	41.0
46	33.225419543621946	35.0	32.0	39.0	22.0	41.0
47	33.13543030497873	35.0	32.0	39.0	21.0	41.0
48	33.061623594653106	35.0	32.0	39.0	21.0	41.0
49	33.04253333218807	35.0	32.0	39.0	20.0	41.0
50	32.89776711049594	35.0	32.0	39.0	20.0	41.0
51	32.67716297749686	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	13.0
13	17.0
14	34.0
15	92.0
16	283.0
17	707.0
18	1591.0
19	3042.0
20	5276.0
21	8253.0
22	12452.0
23	17987.0
24	25697.0
25	35658.0
26	46264.0
27	53109.0
28	56744.0
29	62844.0
30	72738.0
31	88056.0
32	108967.0
33	141978.0
34	242741.0
35	324894.0
36	183890.0
37	227550.0
38	312424.0
39	411493.0
40	60.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.366194533099865	22.328649627647238	28.488759474472765	14.816396364780138
2	32.679594201547005	25.071711701983634	29.69005574554606	12.558638350923305
3	30.392468440920318	24.810387838317055	31.95983899275991	12.837304728002719
4	25.943377511750164	27.277033154059193	31.43817291712937	15.341416417061271
5	23.582341558347537	31.50733846000935	30.217325416312352	14.69299456533076
6	20.51717501909108	41.27959935521844	27.505185370608288	10.698040255082194
7	86.6344030473741	4.193002541250845	7.457526180446399	1.715068230928655
8	87.764243254928	3.2885741059095843	7.022204552491575	1.924978086670847
9	83.7122713416193	4.488111584349036	8.902599291002057	2.8970177830296144
10	49.65002071694114	23.185181640331816	14.7284158309334	12.436381811793645
11	42.487317264513	21.98740295452621	21.25521349083935	14.270066290121433
12	38.63895627518806	21.134879352960642	25.74725168199884	14.47891268985246
13	24.40361591404658	34.266638689593144	25.850447817236088	15.479297579124196
14	18.65281392505662	37.25241414740073	28.639565717286764	15.455206210255886
15	16.613923338728327	23.032862017809617	44.214410728798676	16.138803914663384
16	18.985184830699847	18.145954429273836	41.88208808769749	20.986772652328824
17	19.61340101821823	18.813518489205308	29.28115690925325	32.29192358332321
18	23.978601628969194	22.345092293666017	32.805859151795666	20.870446925569123
19	29.022982511465894	23.9878046136812	25.603889631262987	21.385323243589916
20	30.23978069900964	23.46622034235921	24.06089676337163	22.233102195259523
21	24.49180095866469	26.552328784604757	28.3029000854446	20.65297017128595
22	24.04400417365582	22.791252992503864	27.092155416733643	26.072587417106675
23	20.916339142666303	27.934862501273077	25.803982969979046	25.34481538608157
24	22.24001465933209	24.452453086251598	34.3582595151704	18.949272739245902
25	20.86541596059323	23.97582028247846	32.25637961125775	22.902384145670567
26	19.1432716569749	30.10181773263816	27.13739319936242	23.617517411024522
27	19.712261525102264	28.808000788593535	29.407176446576266	22.072561239727936
28	17.58211823258519	26.419151370283522	35.667496571376915	20.331233825754367
29	18.555957623732084	25.0911811274188	34.44439945207474	21.908461796774375
30	22.038367038753563	26.497724408646878	31.546645430268168	19.91726312233139
31	28.369161575371017	24.664326245399018	25.62564957733759	21.34086260189238
32	28.622632225416684	25.45950502667025	26.074959742054656	19.842903005858414
33	28.03744510419619	25.505806265310188	25.199980857791797	21.25676777270182
34	21.351251749078372	26.12089286130611	29.263323569989108	23.26453181962641
35	21.993538277667547	25.20926564681235	30.59800176615502	22.19919430936508
36	29.534913874378848	24.69438932879156	25.957815972209442	19.81288082462015
37	22.66044790313061	29.879678132767573	27.473199885964796	19.98667407813702
38	23.31725469648761	29.822456018936062	24.001465933209236	22.858823351367093
39	22.720614972069963	27.464774042184025	26.133122605434504	23.681488380311503
40	24.559534926145023	24.552704266381006	26.30343917583795	24.584321631636016
41	20.421995706091845	23.04533717486366	27.52633178436875	29.006335334675743
42	23.626965808662177	25.42019805641143	24.59213394310265	26.360702191823744
43	23.127591407111822	26.171693336916363	25.885909984993	24.81480527097882
44	21.783628421925354	27.87612700773337	27.31494945107264	23.025295119268637
45	20.554845903178876	31.561411107961646	24.267698055388877	23.6160449334706
46	23.714578223120434	28.749837925213683	27.09698187093816	20.43860198072772
47	23.518370589060556	26.680761549030024	27.545392188261165	22.255475673648256
48	23.206328054092282	25.09494412561215	28.695560766490008	23.003167053805555
49	22.43646770631762	23.912749160585538	30.375125927507472	23.27565720558936
50	21.631554212328812	27.762378116693025	27.5953746207859	23.01069305019226
51	21.01143665135699	30.057479797403445	25.385063105888722	23.546020445350834
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1199.0
1	2189.5
2	3180.0
3	13134.5
4	23089.0
5	16917.0
6	10745.0
7	10948.5
8	11152.0
9	11725.5
10	12299.0
11	12214.5
12	12130.0
13	11953.5
14	11777.0
15	11069.5
16	10362.0
17	9947.0
18	9532.0
19	9188.0
20	8844.0
21	8800.5
22	8757.0
23	9000.5
24	9244.0
25	9373.5
26	12542.0
27	15581.0
28	17176.5
29	18772.0
30	22108.0
31	25444.0
32	33726.5
33	42009.0
34	47009.5
35	52010.0
36	58065.5
37	64121.0
38	73212.0
39	82303.0
40	106033.5
41	129764.0
42	156401.0
43	183038.0
44	190696.5
45	198355.0
46	203143.5
47	207932.0
48	205518.0
49	203104.0
50	194043.5
51	184983.0
52	173958.0
53	162933.0
54	149318.5
55	135704.0
56	132876.5
57	130049.0
58	120473.0
59	110897.0
60	102662.5
61	94428.0
62	84522.5
63	74617.0
64	66629.0
65	58641.0
66	52631.0
67	46621.0
68	38070.5
69	29520.0
70	24991.5
71	20463.0
72	17265.5
73	14068.0
74	11425.5
75	6341.0
76	3899.0
77	3176.0
78	2453.0
79	1943.5
80	1434.0
81	981.0
82	528.0
83	434.5
84	341.0
85	241.0
86	141.0
87	96.0
88	51.0
89	35.0
90	19.0
91	15.5
92	12.0
93	16.5
94	21.0
95	13.5
96	6.0
97	3.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2444859.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.343860954226248
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.15043645755604	14.886123227521805
2	7.185092235049669	2.6360466580608364
3	2.448763453206603	1.3475932888623854
4	1.2571592995521863	0.9224462155319108
5	0.7952636661603576	0.7294103056996901
6	0.5605207880217383	0.6169269238454457
7	0.41259742587510656	0.5298040867227238
8	0.34762178439434904	0.5101380542071964
9	0.3031605931477755	0.5005022190753194
>10	3.102688517379232	12.844139098093018
>50	0.9380125959146866	12.483406259478542
>100	1.4847607448901654	48.8445303424609
>500	0.008982218614302359	1.0702542420260186
>1k	0.0044911093071511796	1.245773960495454
>5k	2.24555465357559E-4	0.305522119663882
>10k+	2.24555465357559E-4	0.5273829982549119
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12803	0.52367028119004	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7417	0.3033712782618548	No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	2961	0.12111127880994364	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07509635524993466	0.0
2	0.0	0.0	0.0	0.2614874722836777	0.0
3	0.0	0.0	0.0	0.3624748911900441	0.0
4	0.0	0.0	0.0	0.5065322785485789	0.0
5	0.0	0.0	0.0	0.7784088980182498	0.0
6	0.0	0.0	0.0	1.0539667113727214	0.0
7	0.0	0.0	0.0	1.2141804496701036	0.0
8	0.0	0.0	0.0	1.626596871230611	0.0
9	0.0	0.0	0.0	1.8248496130042673	0.0
10	0.0	0.0	0.0	2.1871199934229337	0.0
11	0.0	0.0	0.0	2.477811603859364	0.0
12	0.0	0.0	0.0	2.766867128124771	0.0
13	0.0	0.0	0.0	2.8782845963714063	0.0
14	0.0	0.0	0.0	2.9249539543998244	0.0
15	0.0	0.0	0.0	2.99939587518135	0.0
16	0.0	0.0	0.0	3.1646405784546268	0.0
17	0.0	0.0	0.0	3.3466142628266087	0.0
18	0.0	0.0	0.0	3.6521124531107927	0.0
19	0.0	0.0	0.0	3.7632845084317745	0.0
20	0.0	0.0	0.0	3.8992841713980235	0.0
21	0.0	0.0	0.0	4.0409283316543	0.0
22	0.0	0.0	0.0	4.180854601431003	0.0
23	0.0	0.0	0.0	4.33088370331377	0.0
24	0.0	0.0	0.0	4.442751095257436	0.0
25	4.090215427556354E-5	0.0	0.0	4.529872683864387	0.0
26	4.090215427556354E-5	0.0	0.0	4.620061934042004	0.0
27	4.090215427556354E-5	0.0	0.0	4.725875807152887	0.0
28	4.090215427556354E-5	0.0	0.0	4.828867431618756	0.0
29	4.090215427556354E-5	0.0	0.0	4.942493616196272	0.0
30	4.090215427556354E-5	0.0	0.0	5.089782273742576	0.0
31	4.090215427556354E-5	0.0	0.0	5.2152700830600045	0.0
32	4.090215427556354E-5	0.0	0.0	5.325133269444168	0.0
33	4.090215427556354E-5	0.0	0.0	5.44211343067228	0.0
34	4.090215427556354E-5	0.0	0.0	5.567887555069638	0.0
35	4.090215427556354E-5	0.0	0.0	5.719389134506326	0.0
36	4.090215427556354E-5	0.0	0.0	5.8355512526489255	0.0
37	4.090215427556354E-5	0.0	0.0	5.963615897685715	0.0
38	4.090215427556354E-5	0.0	0.0	6.101537961902915	0.0
39	4.090215427556354E-5	0.0	0.0	6.298604541202581	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCGT	20	7.0348475E-4	45.000004	11
CAATCGA	20	7.0348475E-4	45.000004	26
ACGGTCC	55	1.8189894E-12	45.000004	10
TATCTCG	35	1.2126839E-7	45.0	1
GACGATT	25	3.8921004E-5	45.0	30
CGTTTCA	25	3.8921004E-5	45.0	26
AGTACGT	25	3.8921004E-5	45.0	5
ATCGCAA	25	3.8921004E-5	45.0	16
CGTTTTT	10270	0.0	42.85297	1
GTACGGG	855	0.0	42.368423	3
CGTTGAT	330	0.0	42.272728	25
TCACGAC	395	0.0	42.1519	25
CGTATGA	70	0.0	41.785713	15
TCGTTGA	335	0.0	41.641792	24
ACAATCG	60	3.6379788E-12	41.249996	15
TAGCCGT	190	0.0	40.263157	44
GTAGGGC	895	0.0	40.223465	4
CTAAGCG	140	0.0	40.17857	1
TATAGCG	90	0.0	40.0	1
ATAACGG	90	0.0	40.0	2
>>END_MODULE